PMID- 30242999
OWN - NLM
STAT- MEDLINE
DCOM- 20190514
LR  - 20190514
IS  - 1110-4902 (Print)
VI  - 25
IP  - 1
DP  - 2018 Jan
TI  - Frequency of Circulating B1a and B2 B-cell Subsets in Egyptian Patients with Type 
      2 Diabetes Mellitus.
PG  - 71-80
AB  - Type 2 diabetes mellitus (T2DM) is a growing health problem in Egypt. T2DM is 
      recognized as chronic inflammatory disease with involvement of immune cells 
      including B cells. We aimed to determine the frequency of antibody secreting B1a 
      and B2 B cells in T2DM patients, their correlation with diabetes metabolic 
      parameters and whether they play a role in diabetic foot infection (DFI) 
      development. This study included 56 participants, recruited from Al-Zahraa 
      hospital, Al-Azhar University, Egypt. Of these, 36 patients were diagnosed with 
      T2DM, divided to two groups; (1) DM group (n=19) recently diagnosed, without foot 
      complication; (2) DFI group (n=17); in addition to a Control group (n=20). The 
      study assessed the frequency of circulating B1a (CD19+CD23-CD5+), and B2 
      (CD19+CD23+CD5-) cells by flow cytometry in diabetic patients. Comparison of the 
      3 studied groups revealed significant differences in frequency of studied total B 
      cells (P=0.011), B1a (P < 0.001) and B2 subsets (P < 0.001). Comparison of B cell 
      subsets between DFI, DM groups showed significant decrease in B1a in DFI group (P 
      < 0.001). B1a cells % showed inverse correlation with HgA1c (r=-0.47, P < 0.001), 
      LDL (r=-0.64, P < 0.001), and TG (r=-0.67, P < 0.001) but showed positive 
      correlation with HDL (r=0.61, P < 0.001), while B2 cells showed opposite 
      correlations. We concluded that imbalance of B cell subsets is seen in T2DM 
      subjects. Beneficial role of B1a cells was spotted as they correlated inversely 
      with glycemia and lipidemia in contrary to B2 cells. Decrease in B1a cells may 
      predispose to DFI development.
CI  - Copyright(c) by the Egyptian Association of Immunologists.
FAU - Hammad, Reham H
AU  - Hammad RH
AD  - Department of Clinical Pathology, Faculty of Medicine for girls, Al-Azhar 
      University, Cairo, Egypt.
FAU - El-Madbouly, Asmaa A
AU  - El-Madbouly AA
AD  - Department of Clinical Pathology, Faculty of Medicine for girls, Al-Azhar 
      University, Cairo, Egypt.
FAU - Kotb, Hend G
AU  - Kotb HG
AD  - Department of Internal Medicine, Faculty of Medicine for girls, Al-Azhar 
      University, Cairo, Egypt.
FAU - Zarad, Mohammed S
AU  - Zarad MS
AD  - Department of Surgery, Faculty of Medicine for girls, Al-Azhar University, Cairo, 
      Egypt.
LA  - eng
PT  - Journal Article
PL  - Egypt
TA  - Egypt J Immunol
JT  - The Egyptian journal of immunology
JID - 9816016
SB  - IM
MH  - B-Lymphocyte Subsets/*cytology
MH  - Case-Control Studies
MH  - Diabetes Mellitus, Type 2/*immunology
MH  - Diabetic Foot/*immunology
MH  - Egypt
MH  - Flow Cytometry
MH  - Humans
EDAT- 2018/09/23 06:00
MHDA- 2019/05/15 06:00
CRDT- 2018/09/23 06:00
PHST- 2018/09/23 06:00 [entrez]
PHST- 2018/09/23 06:00 [pubmed]
PHST- 2019/05/15 06:00 [medline]
PST - ppublish
SO  - Egypt J Immunol. 2018 Jan;25(1):71-80.