PMID- 30244244
OWN - NLM
STAT- MEDLINE
DCOM- 20181016
LR  - 20220630
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 49
IP  - 5
DP  - 2018
TI  - Ligustrazine Inhibits Growth, Migration and Invasion of Medulloblastoma Daoy 
      Cells by Up-Regulation of miR-211.
PG  - 2012-2021
LID - 10.1159/000493712 [doi]
AB  - BACKGROUND/AIMS: Ligustrazine (LSZ) has been identified as an antitumor agent 
      against some types of cancers. Nevertheless, its ability to inhibit growth, 
      migration and invasion of medulloblastoma cells is still unclear. This study 
      aimed to explore the effect of LSZ on Daoy cells. METHODS: The effects of LSZ on 
      viability, proliferation, apoptosis, migration, and invasion of Daoy cells were 
      analyzed by CCK-8, BrdU, flow cytometry and Transwell assays, respectively. The 
      effect of LSZ on miR-211 expression was analyzed by qRT-PCR. miR-211 inhibitor 
      transfection was performed to suppress miR-211 expression. The effects of LSZ on 
      apoptosis-related factors, MMP-2, MMP-9, and Vimentin (Vim), as well as main 
      factors of PI3K/AKT and mTOR pathways were analyzed by Western blot. RESULTS: LSZ 
      inhibited viability but promoted apoptosis of Daoy cells. Additionally, the 
      proliferative, migratory and invasive abilities of Daoy cells were decreased by 
      LSZ. Meanwhile, LSZ promoted the activations of Caspase-3 and Caspase-9, 
      increased Bax level, decreased Bcl-2 level, as well as inhibited the expressions 
      of MMP-2, MMP-9 and Vim. Additionally, we found that LSZ enhanced miR-211 
      expression and exerted its anti-medulloblastoma effect by up-regulation of 
      miR-211. Furthermore, LSZ inhibited PI3K/AKT and mTOR signaling pathways by 
      up-regulating miR-211. CONCLUSION: LSZ suppressed medulloblastoma Daoy cells by 
      up-regulating miR-211 and further modulating the activations of PI3K/AKT and mTOR 
      signaling pathways.
CI  - (c) 2018 The Author(s). Published by S. Karger AG, Basel.
FAU - Xu, Donghui
AU  - Xu D
FAU - Chi, Guonan
AU  - Chi G
FAU - Zhao, Conghai
AU  - Zhao C
FAU - Li, Dongyuan
AU  - Li D
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
DEP - 20180921
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (Antagomirs)
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (MIRN211 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Pyrazines)
RN  - 0 (Vasodilator Agents)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - V80F4IA5XG (tetramethylpyrazine)
SB  - IM
RIN - Cell Physiol Biochem. 2022 Jun 30;56(3):324. PMID: 35771826
MH  - Antagomirs/metabolism
MH  - Apoptosis Regulatory Proteins/metabolism
MH  - Caspase 3/metabolism
MH  - Cell Line, Tumor
MH  - Cell Movement/drug effects
MH  - Cell Proliferation/*drug effects
MH  - Cerebellar Neoplasms/metabolism/pathology
MH  - Humans
MH  - Matrix Metalloproteinase 2/metabolism
MH  - Medulloblastoma/metabolism/pathology
MH  - MicroRNAs/antagonists & inhibitors/genetics/*metabolism
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Pyrazines/chemistry/*pharmacology
MH  - Signal Transduction/drug effects
MH  - TOR Serine-Threonine Kinases/metabolism
MH  - Up-Regulation/*drug effects
MH  - Vasodilator Agents/chemistry/*pharmacology
OTO - NOTNLM
OT  - Ligustrazine
OT  - Medulloblastoma
OT  - PI3K/AKT pathway
OT  - mTOR pathway
OT  - miR-211
EDAT- 2018/09/24 06:00
MHDA- 2018/10/17 06:00
CRDT- 2018/09/24 06:00
PHST- 2018/01/15 00:00 [received]
PHST- 2018/09/13 00:00 [accepted]
PHST- 2018/09/24 06:00 [pubmed]
PHST- 2018/10/17 06:00 [medline]
PHST- 2018/09/24 06:00 [entrez]
AID - 000493712 [pii]
AID - 10.1159/000493712 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2018;49(5):2012-2021. doi: 10.1159/000493712. Epub 2018 Sep 
      21.