PMID- 30244396
OWN - NLM
STAT- MEDLINE
DCOM- 20190424
LR  - 20221207
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Print)
IS  - 0301-4851 (Linking)
VI  - 45
IP  - 6
DP  - 2018 Dec
TI  - Constructing and transient expression of a gene cassette containing edible 
      vaccine elements and shigellosis, anthrax and cholera recombinant antigens in 
      tomato.
PG  - 2237-2246
LID - 10.1007/s11033-018-4385-3 [doi]
AB  - Shigella dysenteriae causing shigellosis is one of the diseases that threaten the 
      health of human society in the developing countries. In Shigella, IpaD gene is 
      one of the key pathogenic genes causing strong mucosal immune system reactions. 
      Anthrax disease is caused by Bacillus anthracis. PA protective antigen is one of 
      the subunits in anthrax toxin complex responsible for the transfer of other 
      subunits into the cytosol of host cells. The 20 kDa subunit of PA (PA20) has the 
      property of immunogenicity. CTxB or B subunit of Vibrio cholerae toxin (CT) is a 
      non-toxic protein and has the function to transfer toxic subunit into cytosol of 
      the host cells by binding to GM1 receptor. The aim of this study was to fuse 
      PA20, ipaD and CTxB and transform tomato plants by this cassette in order to 
      produce an oral vaccine against shigellosis, anthrax and cholera. CTxB was used 
      for these two antigens as an immune adjuvant. IpaD and PA20 genes were cloned in 
      pBI121 containing the CTxB gene and Extensin signal peptide. In order to evaluate 
      the transient expression of Shigellosis, Anthrax and Cholera antigens, 
      agro-infiltrated tomato tissues were inoculated with Agrobacterium tumefaciens 
      containing the gene cassette. Cloning was confirmed by PCR, enzymatic digestion 
      and sequencing techniques. Expression of the antigens was examined by SDS-PAGE, 
      dot blot and ELISA. Maturate green fruits demonstrated the highest expression of 
      the recombinant proteins. The first phase of this study was carried out for 
      cloning and expressing of CtxB, ipaD and PA20 antigens in tomato. In the next 
      phase, we aim to analyze the immunogenicity of this vaccine candidate in 
      laboratory animals.
FAU - Davod, Jafari
AU  - Davod J
AD  - Medical Biotechnology Department, School of Allied Medicine, Iran University of 
      Medical Sciences, Tehran, Iran.
AD  - Biotechnology Department, Faculty of Agricultural and Natural Sciences, Imam 
      Khomeini International University (IKIU), Qazvin, Iran.
FAU - Fatemeh, Dehghan Nayeri
AU  - Fatemeh DN
AD  - Biotechnology Department, Faculty of Agricultural and Natural Sciences, Imam 
      Khomeini International University (IKIU), Qazvin, Iran. nayeri@ut.ac.ir.
FAU - Honari, Hossein
AU  - Honari H
AD  - Faculty of Basic Science, Imam Hussein University, Tehran, Iran.
FAU - Hosseini, Ramin
AU  - Hosseini R
AD  - Biotechnology Department, Faculty of Agricultural and Natural Sciences, Imam 
      Khomeini International University (IKIU), Qazvin, Iran.
LA  - eng
PT  - Journal Article
DEP - 20180922
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Bacterial Toxins)
RN  - 0 (IpaD protein, Shigella flexneri)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Vaccines)
RN  - 0 (Vaccines, Edible)
RN  - 0 (anthrax toxin)
SB  - IM
MH  - Agrobacterium tumefaciens/genetics
MH  - Animals
MH  - Anthrax
MH  - Antigens, Bacterial/genetics
MH  - Bacillus anthracis/genetics
MH  - Bacterial Proteins/genetics
MH  - Bacterial Toxins
MH  - Cholera
MH  - Dysentery, Bacillary
MH  - Genetic Engineering/methods
MH  - Genetic Vectors
MH  - Humans
MH  - Solanum lycopersicum/*genetics
MH  - Recombinant Proteins/genetics
MH  - Vaccines/genetics
MH  - Vaccines, Edible/*biosynthesis/*genetics
PMC - PMC7088786
OTO - NOTNLM
OT  - Agro-infiltration
OT  - Anthrax
OT  - Cholera
OT  - Edible vaccine
OT  - Shigellosis
OT  - Tomato
COIS- The authors declare that they have no competing interests.
EDAT- 2018/09/24 06:00
MHDA- 2019/04/25 06:00
PMCR- 2020/03/23
CRDT- 2018/09/24 06:00
PHST- 2018/08/11 00:00 [received]
PHST- 2018/09/12 00:00 [accepted]
PHST- 2018/09/24 06:00 [pubmed]
PHST- 2019/04/25 06:00 [medline]
PHST- 2018/09/24 06:00 [entrez]
PHST- 2020/03/23 00:00 [pmc-release]
AID - 10.1007/s11033-018-4385-3 [pii]
AID - 4385 [pii]
AID - 10.1007/s11033-018-4385-3 [doi]
PST - ppublish
SO  - Mol Biol Rep. 2018 Dec;45(6):2237-2246. doi: 10.1007/s11033-018-4385-3. Epub 2018 
      Sep 22.