PMID- 30245613
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240331
IS  - 1662-1018 (Print)
IS  - 1662-100X (Electronic)
IS  - 1662-100X (Linking)
VI  - 16
DP  - 2012
TI  - Osteocyte Characterization on Polydimethylsiloxane Substrates for Microsystems 
      Applications.
PG  - 27-42
LID - 10.4028/www.scientific.net/JBBTE.16.27 [doi]
AB  - In the body, osteocytes reside in lacunae, lenticular shaped cavities within 
      mineralized bone. These cells are linked to each other and surface-residing 
      osteoblasts via physical channels known as gap junctions. It has been suggested 
      that osteocytes sense mechanical load applied to bone and relay that signal to 
      osteoclasts and osteoblasts. Current in vitro and in vivo models of 
      mechanotransduction face temporal and spatial barriers. Recent advances in 
      polydimethylsiloxane (PDMS) based microfabrication techniques may be able to 
      overcome some of these hurdles. However, before the bone research field can 
      effectively utilize microsystems techniques, fundamental groundwork must be 
      completed. This study characterized the behaviour of osteocytes on PDMS coated 
      with collagen type I (CTI) and provides the framework for bone cell 
      mechanotransduction studies using microsystems. The goal was to determine whether 
      osteocytes were adversely affected by the substrate material by comparing their 
      behaviour to a standard glass substrate. In addition, optimal culture conditions 
      and time points for growing osteocytes on PDMS substrates were determined. 
      Results of this study suggested that use of PDMS does not adversely affect 
      osteocyte behaviour. Furthermore, the results demonstrated that osteocytes should 
      be cultured for no less than 72 hours prior to experimentation to allow the 
      establishment and maintenance of phenotypic characteristics. These results 
      completed essential groundwork necessary for further studies regarding osteocytes 
      in microsystems modelling utilizing PDMS.
FAU - York, Spencer L
AU  - York SL
AD  - Department of Biomedical Engineering, The University of Akron, 260 S Forge St, 
      Akron, OH 44325, USA.
FAU - Arida, Ahmad R
AU  - Arida AR
AD  - Department of Biomedical Engineering, The University of Akron, 260 S Forge St, 
      Akron, OH 44325, USA.
FAU - Shah, Karan S
AU  - Shah KS
AD  - Department of Biomedical Engineering, The University of Akron, 260 S Forge St, 
      Akron, OH 44325, USA.
FAU - Sethu, Palaniappan
AU  - Sethu P
AD  - Department of Bioengineering, University of Louisville, 2100 S Brook St, SRB 357, 
      Louisville, KY, 40292, USA.
FAU - Saunders, Marnie M
AU  - Saunders MM
AD  - Department of Biomedical Engineering, The University of Akron, 260 S Forge St, 
      Akron, OH 44325, USA.
LA  - eng
GR  - R15 DE022664/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PL  - Switzerland
TA  - J Biomim Biomater Tissue Eng
JT  - Journal of biomimetics, biomaterials, and tissue engineering
JID - 101469193
PMC - PMC6150457
MID - NIHMS905282
OTO - NOTNLM
OT  - Osteocytes
OT  - PDMS
OT  - gap junctions
OT  - sclerostin
EDAT- 2012/01/01 00:00
MHDA- 2012/01/01 00:01
PMCR- 2018/09/21
CRDT- 2018/09/25 06:00
PHST- 2018/09/25 06:00 [entrez]
PHST- 2012/01/01 00:00 [pubmed]
PHST- 2012/01/01 00:01 [medline]
PHST- 2018/09/21 00:00 [pmc-release]
AID - 10.4028/www.scientific.net/JBBTE.16.27 [doi]
PST - ppublish
SO  - J Biomim Biomater Tissue Eng. 2012;16:27-42. doi: 
      10.4028/www.scientific.net/JBBTE.16.27.