PMID- 30248951 OWN - NLM STAT- MEDLINE DCOM- 20190107 LR - 20190107 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 19 IP - 10 DP - 2018 Sep 21 TI - Polydopamine-Assisted Silver Nanoparticle Self-Assembly on Sericin/Agar Film for Potential Wound Dressing Application. LID - 10.3390/ijms19102875 [doi] LID - 2875 AB - Silver nanoparticles (AgNPs) are extensively applied for their broad-spectrum and excellent antibacterial ability in recent years. Polydopamine (PDA) has great advantages for synthesizing large amounts of AgNPs, as it has multiple sites for silver ion binding and phenolic hydroxyl structure to reduce silver ions to AgNPs. Here, we mixed sericin and agar solution and dried at 65 degrees C to prepare a sericin (SS)/Agar composite film, and then coated polydopamine (PDA) on the surface of SS/Agar film by soaking SS/Agar film into polydopamine solution, subsequently synthesizing high-density AgNPs with the assistance of PDA to yield antibacterial AgNPs-PDA- SS/Agar film. Scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) spectra indicated the successful synthesis of high-density AgNPs on the surface of PDA-SS/Agar film. PDA coating and AgNPs modification did not affect the structure of sericin and agar. Furthermore, water contact angle, water absorption and mechanical property analysis showed that AgNPs-PDA-SS/Agar film had excellent hydrophilicity and proper mechanical properties. Inhibition zone and growth curve assays suggested the prepared film had excellent and long-lasting antibacterial ability. In addition, it had excellent cytocompatibility on the fibroblast NIH/3T3 cells. The film shows great potential as a novel kind of wound dressing. FAU - Liu, Liying AU - Liu L AD - State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China. l3341345@email.swu.edu.cn. FAU - Cai, Rui AU - Cai R AD - College of Biotechnology, Southwest University, Chongqing 400715, China. cairui0330@email.swu.edu.cn. FAU - Wang, Yejing AU - Wang Y AD - State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China. yjwang@swu.edu.cn. AD - College of Biotechnology, Southwest University, Chongqing 400715, China. yjwang@swu.edu.cn. FAU - Tao, Gang AU - Tao G AD - State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China. taogang@email.swu.edu.cn. FAU - Ai, Lisha AU - Ai L AD - State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China. als123@email.swu.edu.cn. FAU - Wang, Peng AU - Wang P AD - College of Biotechnology, Southwest University, Chongqing 400715, China. modelsums@email.swu.edu.cn. FAU - Yang, Meirong AU - Yang M AD - State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China. yangmeirong@email.swu.edu.cn. FAU - Zuo, Hua AU - Zuo H AUID- ORCID: 0000-0002-8461-8988 AD - College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China. zuohua@swu.edu.cn. FAU - Zhao, Ping AU - Zhao P AD - State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China. zhaop@swu.edu.cn. AD - Chongqing Key Laboratory of Sericultural Science, Chongqing Engineering and Technology Research Center for Novel Silk Materials, Southwest University, Chongqing 400715, China. zhaop@swu.edu.cn. FAU - He, Huawei AU - He H AUID- ORCID: 0000-0002-3805-8940 AD - State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China. hehuawei@swu.edu.cn. AD - Chongqing Key Laboratory of Sericultural Science, Chongqing Engineering and Technology Research Center for Novel Silk Materials, Southwest University, Chongqing 400715, China. hehuawei@swu.edu.cn. LA - eng GR - 31572465/National Natural Science Foundation of China/ GR - 31530071/National Natural Science Foundation of China/ GR - cstc2015jcyjBX0035/Chongqing Research Program of Basic Research and Frontier Technology/ GR - XDJK2018B010, XDJK2018C063/Fundamental Research Funds for the Central Universities/ GR - CYB17069, CYS18123/Graduate Research and Innovation Project of Chongqing/ GR - silkgczx2016003/Open Project Program of Chongqing Engineering and Technology Research Center for Novel Silk Materials/ PT - Journal Article DEP - 20180921 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Anti-Bacterial Agents) RN - 0 (Indoles) RN - 0 (Polymers) RN - 0 (Sericins) RN - 0 (polydopamine) RN - 3M4G523W1G (Silver) RN - 9002-18-0 (Agar) SB - IM MH - Agar/chemistry MH - Animals MH - Anti-Bacterial Agents/chemistry/pharmacology MH - Escherichia coli/drug effects MH - Indoles/*chemistry/pharmacology MH - Metal Nanoparticles/*chemistry MH - Mice MH - Microbial Sensitivity Tests MH - Microscopy, Electron, Scanning MH - NIH 3T3 Cells MH - Polymers/*chemistry/pharmacology MH - Sericins/*chemistry MH - Silver/*chemistry MH - Spectroscopy, Fourier Transform Infrared MH - Staphylococcus aureus/drug effects MH - Wettability MH - X-Ray Diffraction PMC - PMC6213261 OTO - NOTNLM OT - antimicrobial activity OT - cytocompatibility OT - polydopamine OT - sericin OT - silver nanoparticle COIS- The authors declare no conflict of interest. The founding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results. EDAT- 2018/09/27 06:00 MHDA- 2019/01/08 06:00 PMCR- 2018/10/01 CRDT- 2018/09/26 06:00 PHST- 2018/08/23 00:00 [received] PHST- 2018/09/16 00:00 [revised] PHST- 2018/09/19 00:00 [accepted] PHST- 2018/09/26 06:00 [entrez] PHST- 2018/09/27 06:00 [pubmed] PHST- 2019/01/08 06:00 [medline] PHST- 2018/10/01 00:00 [pmc-release] AID - ijms19102875 [pii] AID - ijms-19-02875 [pii] AID - 10.3390/ijms19102875 [doi] PST - epublish SO - Int J Mol Sci. 2018 Sep 21;19(10):2875. doi: 10.3390/ijms19102875.