PMID- 30254669
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20181211
IS  - 1687-5443 (Electronic)
IS  - 2090-5904 (Print)
IS  - 1687-5443 (Linking)
VI  - 2018
DP  - 2018
TI  - Botulinum Neurotoxin Application to the Severed Femoral Nerve Modulates Spinal 
      Synaptic Responses to Axotomy and Enhances Motor Recovery in Rats.
PG  - 7975013
LID - 10.1155/2018/7975013 [doi]
LID - 7975013
AB  - Botulinum neurotoxin A (BoNT) and brain-derived neurotrophic factor (BDNF) are 
      known for their ability to influence synaptic inputs to neurons. Here, we tested 
      if these drugs can modulate the deafferentation of motoneurons following nerve 
      section/suture and, as a consequence, modify the outcome of peripheral nerve 
      regeneration. We applied drug solutions to the proximal stump of the freshly cut 
      femoral nerve of adult rats to achieve drug uptake and transport to the neuronal 
      perikarya. The most marked effect of this application was a significant reduction 
      of the axotomy-induced loss of perisomatic cholinergic terminals by BoNT at one 
      week and two months post injury. The attenuation of the synaptic deficit was 
      associated with enhanced motor recovery of the rats 2-20 weeks after injury. 
      Although BDNF also reduced cholinergic terminal loss at 1 week, it had no effect 
      on this parameter at two months and no effect on functional recovery. These 
      findings strengthen the idea that persistent partial deafferentation of 
      axotomized motoneurons may have a significant negative impact on functional 
      outcome after nerve injury. Intraneural application of drugs may be a promising 
      way to modify deafferentation and, thus, elucidate relationships between synaptic 
      plasticity and restoration of function.
FAU - Irintchev, Marcel
AU  - Irintchev M
AD  - Department of Otorhinolaryngology, Jena University Hospital, Am Klinikum 1, 07747 
      Jena, Germany.
FAU - Guntinas-Lichius, Orlando
AU  - Guntinas-Lichius O
AUID- ORCID: 0000-0001-9671-0784
AD  - Department of Otorhinolaryngology, Jena University Hospital, Am Klinikum 1, 07747 
      Jena, Germany.
FAU - Irintchev, Andrey
AU  - Irintchev A
AUID- ORCID: 0000-0002-4637-1259
AD  - Department of Otorhinolaryngology, Jena University Hospital, Am Klinikum 1, 07747 
      Jena, Germany.
LA  - eng
PT  - Journal Article
DEP - 20180905
PL  - United States
TA  - Neural Plast
JT  - Neural plasticity
JID - 100883417
RN  - EC 3.4.24.69 (Botulinum Toxins, Type A)
SB  - IM
MH  - Animals
MH  - Axons/drug effects
MH  - Axotomy
MH  - Botulinum Toxins, Type A/*administration & dosage
MH  - Female
MH  - Femoral Nerve/*drug effects/injuries
MH  - Motor Neurons/drug effects
MH  - Nerve Regeneration/*drug effects
MH  - Rats
MH  - Rats, Wistar
MH  - Recovery of Function/*drug effects
MH  - Synapses/drug effects
PMC - PMC6145158
EDAT- 2018/09/27 06:00
MHDA- 2018/12/12 06:00
PMCR- 2018/09/05
CRDT- 2018/09/27 06:00
PHST- 2018/03/06 00:00 [received]
PHST- 2018/07/15 00:00 [revised]
PHST- 2018/08/05 00:00 [accepted]
PHST- 2018/09/27 06:00 [entrez]
PHST- 2018/09/27 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
PHST- 2018/09/05 00:00 [pmc-release]
AID - 10.1155/2018/7975013 [doi]
PST - epublish
SO  - Neural Plast. 2018 Sep 5;2018:7975013. doi: 10.1155/2018/7975013. eCollection 
      2018.