PMID- 30254680
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1687-966X (Print)
IS  - 1687-9678 (Electronic)
VI  - 2018
DP  - 2018
TI  - 3D Culture of Bone Marrow-Derived Mesenchymal Stem Cells (BMSCs) Could Improve 
      Bone Regeneration in 3D-Printed Porous Ti6Al4V Scaffolds.
PG  - 2074021
LID - 10.1155/2018/2074021 [doi]
LID - 2074021
AB  - Mandibular bone defect reconstruction is an urgent challenge due to the 
      requirements for daily eating and facial aesthetics. Three-dimensional- (3D-) 
      printed titanium (Ti) scaffolds could provide patient-specific implants for bone 
      defects. Appropriate load-bearing properties are also required during bone 
      reconstruction, which makes them potential candidates for mandibular bone defect 
      reconstruction implants. However, in clinical practice, the insufficient 
      osteogenesis of the scaffolds needs to be further improved. In this study, we 
      first encapsulated bone marrow-derived mesenchymal stem cells (BMSCs) into 
      Matrigel. Subsequently, the BMSC-containing Matrigels were infiltrated into 
      porous Ti6Al4V scaffolds. The Matrigels in the scaffolds provided a 3D culture 
      environment for the BMSCs, which was important for osteoblast differentiation and 
      new bone formation. Our results showed that rats with a full thickness of 
      critical mandibular defects treated with Matrigel-infiltrated Ti6Al4V scaffolds 
      exhibited better new bone formation than rats with local BMSC injection or 
      Matrigel-treated defects. Our data suggest that Matrigel is able to create a more 
      favorable 3D microenvironment for BMSCs, and Matrigel containing infiltrated 
      BMSCs may be a promising method for enhancing the bone formation properties of 
      3D-printed Ti6Al4V scaffolds. We suggest that this approach provides an 
      opportunity to further improve the efficiency of stem cell therapy for the 
      treatment of mandibular bone defects.
FAU - Yu, Lingjia
AU  - Yu L
AD  - Department of Orthopaedic Surgery, Peking Union Medical College Hospital, Peking 
      Union Medical College and Chinese Academy of Medical Sciences, Beijing 100730, 
      China.
FAU - Wu, Yuanhao
AU  - Wu Y
AD  - Central Laboratory, Peking Union Medical College Hospital, Peking Union Medical 
      College and Chinese Academy of Medical Sciences, Beijing 100730, China.
FAU - Liu, Jieying
AU  - Liu J
AD  - Central Laboratory, Peking Union Medical College Hospital, Peking Union Medical 
      College and Chinese Academy of Medical Sciences, Beijing 100730, China.
FAU - Li, Bo
AU  - Li B
AD  - Department of Orthopaedic Surgery, Peking Union Medical College Hospital, Peking 
      Union Medical College and Chinese Academy of Medical Sciences, Beijing 100730, 
      China.
AD  - Department of Orthopaedic Surgery, Fourth Clinical Medical College of Peking 
      University, Beijing Jishuitan Hospital, Beijing 100035, China.
FAU - Ma, Bupeng
AU  - Ma B
AD  - Central Laboratory, Peking Union Medical College Hospital, Peking Union Medical 
      College and Chinese Academy of Medical Sciences, Beijing 100730, China.
FAU - Li, Yaqian
AU  - Li Y
AD  - Central Laboratory, Peking Union Medical College Hospital, Peking Union Medical 
      College and Chinese Academy of Medical Sciences, Beijing 100730, China.
FAU - Huang, Zhenfei
AU  - Huang Z
AD  - Department of Orthopaedic Surgery, Peking Union Medical College Hospital, Peking 
      Union Medical College and Chinese Academy of Medical Sciences, Beijing 100730, 
      China.
FAU - He, Yu
AU  - He Y
AD  - Department of Orthopaedic Surgery, Peking Union Medical College Hospital, Peking 
      Union Medical College and Chinese Academy of Medical Sciences, Beijing 100730, 
      China.
FAU - Wang, Hai
AU  - Wang H
AUID- ORCID: 0000-0003-1453-855X
AD  - Department of Orthopaedic Surgery, Peking Union Medical College Hospital, Peking 
      Union Medical College and Chinese Academy of Medical Sciences, Beijing 100730, 
      China.
FAU - Wu, Zhihong
AU  - Wu Z
AUID- ORCID: 0000-0002-1902-4198
AD  - Central Laboratory, Peking Union Medical College Hospital, Peking Union Medical 
      College and Chinese Academy of Medical Sciences, Beijing 100730, China.
AD  - Beijing Key Laboratory for Genetic Research of Bone and Joint Disease, Beijing, 
      China.
FAU - Qiu, Guixing
AU  - Qiu G
AD  - Department of Orthopaedic Surgery, Peking Union Medical College Hospital, Peking 
      Union Medical College and Chinese Academy of Medical Sciences, Beijing 100730, 
      China.
LA  - eng
GR  - K23 AA020316/AA/NIAAA NIH HHS/United States
PT  - Journal Article
DEP - 20180905
PL  - United States
TA  - Stem Cells Int
JT  - Stem cells international
JID - 101535822
PMC - PMC6145055
EDAT- 2018/09/27 06:00
MHDA- 2018/09/27 06:01
PMCR- 2018/09/05
CRDT- 2018/09/27 06:00
PHST- 2018/01/21 00:00 [received]
PHST- 2018/07/10 00:00 [revised]
PHST- 2018/07/30 00:00 [accepted]
PHST- 2018/09/27 06:00 [entrez]
PHST- 2018/09/27 06:00 [pubmed]
PHST- 2018/09/27 06:01 [medline]
PHST- 2018/09/05 00:00 [pmc-release]
AID - 10.1155/2018/2074021 [doi]
PST - epublish
SO  - Stem Cells Int. 2018 Sep 5;2018:2074021. doi: 10.1155/2018/2074021. eCollection 
      2018.