PMID- 30257250
OWN - NLM
STAT- MEDLINE
DCOM- 20181023
LR  - 20220408
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 49
IP  - 6
DP  - 2018
TI  - Schizandrin A Alleviates LPS-Induced Injury in Human Keratinocyte Cell Hacat 
      Through a MicroRNA-127-Dependent Regulation.
PG  - 2229-2239
LID - 10.1159/000493826 [doi]
AB  - BACKGROUND/AIMS: Inflammatory skin diseases are the most common problems in 
      dermatology. Schizandrin A (SchA) has been reported to have anti-inflammatory 
      properties. Herein, we aimed to investigate the protective effects of SchA on 
      lipopolysaccharide (LPS)-induced injury in keratinocyte HaCaT cells. METHODS: 
      Inflammation injury in HaCaT cells was induced by LPS treatment. Cell viability, 
      apoptotic cell rate, and apoptosis-related proteins were analyzed by cell 
      counting kit-8 (CCK-8) assay, Annexin V-(fluorescein isothiocyanate (FITC)/ 
      Propidium Iodide (PI) double staining method, and western blot, respectively. The 
      pro-inflammatory factors were analyzed by western blot and quantified by enzyme 
      linked immunosorbent assay (ELISA). Expression of miR-127 in SchA-treated cells 
      was analyzed by qRT-PCR. The effects of SchA on activations of p38MAPK/ERK and 
      JNK pathways were analyzed by western blot. RESULTS: SchA protected HaCaT cells 
      from LPS-induced inflammation damage via promoting cell viability, suppressing 
      apoptosis. Meanwhile, SchA inhibited IL-1beta, IL-6, and TNF-alpha expression. miR-127 
      expression was up-regulated in LPS-treated HaCaT cells but down-regulated after 
      SchA treatment. Overexpression of miR-127 inhibited cell growth and induced 
      expression of IL-1beta, IL-6 and TNF-alpha. Additionally, miR-127 overexpression 
      impaired the protective effects of SchA, implying miR-127 might be correlated to 
      the anti-inflammation property of SchA and also involved in inactivation of 
      p38MAPK/ERK and JNK pathways by SchA. CONCLUSION: miR-127 is involved in the 
      protective functions of SchA on LPS-induced inflammation injury in human 
      keratinocyte cell HaCaT, which might inactivates of p38MAPK/ERK and JNK signaling 
      pathways in HaCaT cells.
CI  - (c) 2018 The Author(s). Published by S. Karger AG, Basel.
FAU - Li, Shujie
AU  - Li S
AD  - Department of Rheumatology and Immunology, Jining No. 1 People's Hospital, 
      Jining, China.
FAU - Xie, Ruijin
AU  - Xie R
AD  - Department of Gastroenterology, Jining No. 1 People's Hospital, Jining, China.
FAU - Jiang, Chengrui
AU  - Jiang C
AD  - Department of Rheumatology and Immunology, Jining No. 1 People's Hospital, 
      Jining, China.
FAU - Liu, Mei
AU  - Liu M
AD  - Department of Gastroenterology, Jining No. 1 People's Hospital, Jining, China.
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
DEP - 20180926
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (Antagomirs)
RN  - 0 (Cyclooctanes)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (Lignans)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (MIRN127 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Polycyclic Compounds)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 74XQL5DO3S (schizandrin A)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
ECI - Cell Physiol Biochem. 2021;55(4):536. PMID: 34735052
RIN - Cell Physiol Biochem. 2022 Feb 26;56(1):85. PMID: 35235275
MH  - Antagomirs/metabolism
MH  - Apoptosis/*drug effects
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Cyclooctanes/*pharmacology
MH  - Humans
MH  - Interleukin-1beta/metabolism
MH  - Interleukin-6/metabolism
MH  - JNK Mitogen-Activated Protein Kinases/metabolism
MH  - Keratinocytes/cytology/drug effects/metabolism
MH  - Lignans/*pharmacology
MH  - Lipopolysaccharides/toxicity
MH  - MAP Kinase Signaling System/drug effects
MH  - MicroRNAs/antagonists & inhibitors/genetics/*metabolism
MH  - Polycyclic Compounds/*pharmacology
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
OTO - NOTNLM
OT  - Inflammation
OT  - JNK pathway
OT  - P38MAPK/ERK pathway
OT  - Schizandrin A
OT  - miR-127
EDAT- 2018/09/27 06:00
MHDA- 2018/10/24 06:00
CRDT- 2018/09/27 06:00
PHST- 2017/11/29 00:00 [received]
PHST- 2018/09/18 00:00 [accepted]
PHST- 2018/09/27 06:00 [pubmed]
PHST- 2018/10/24 06:00 [medline]
PHST- 2018/09/27 06:00 [entrez]
AID - 000493826 [pii]
AID - 10.1159/000493826 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2018;49(6):2229-2239. doi: 10.1159/000493826. Epub 2018 Sep 
      26.