PMID- 30257422 OWN - NLM STAT- MEDLINE DCOM- 20190111 LR - 20231004 IS - 1660-3397 (Electronic) IS - 1660-3397 (Linking) VI - 16 IP - 10 DP - 2018 Sep 25 TI - Evaluation of Differentiated Bone Cells Proliferation by Blue Shark Skin Collagen via Biochemical for Bone Tissue Engineering. LID - 10.3390/md16100350 [doi] LID - 350 AB - Collagen from a marine resource is believed to have more potential activity in bone tissue engineering and their bioactivity depends on biochemical and structural properties. Considering the above concept, pepsin soluble collagen (PSC) and acid soluble collagen (ASC) from blue shark (Prionace glauca) skin were extracted and its biochemical and osteogenic properties were investigated. The hydroxyproline content was higher in PSC than ASC and the purified collagens contained three distinct bands alpha(1), alpha(2,) and beta dimer. The purity of collagen was confirmed by the RP-HPLC profile and the thermogravimetric data showed a two-step thermal degradation pattern. ASC had a sharp decline in viscosity at 20(-)30 degrees C. Scanning electron microscope (SEM) images revealed the fibrillar network structure of collagens. Proliferation rates of the differentiated mouse bone marrow-mesenchymal stem (dMBMS) and differentiated osteoblastic (dMC3T3E1) cells were increased in collagen treated groups rather than the controls and the effect was dose-dependent, which was further supported by higher osteogenic protein and mRNA expression in collagen treated bone cells. Among two collagens, PSC had significantly increased dMBMS cell proliferation and this was materialized through increasing RUNX2 and collagen-I expression in bone cells. Accordingly, the collagens from blue shark skin with excellent biochemical and osteogenic properties could be a suitable biomaterial for therapeutic application. FAU - Elango, Jeevithan AU - Elango J AD - Department of Marine Bio-Pharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China. srijeevithan@gmail.com. FAU - Lee, Jung Woo AU - Lee JW AD - Department of Marine Bio-Pharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China. truthwo@naver.com. AD - Division of Marine Bioscience, Korea Maritime and Ocean University, Busan 606791, Korea. truthwo@naver.com. FAU - Wang, Shujun AU - Wang S AD - Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Huaihai Institute of Technology, Lianyungang 222005, China. shujunwang86@163.com. FAU - Henrotin, Yves AU - Henrotin Y AD - Bone and Cartilage Research Unit, Arthropole Liege, University of Liege, CHU Sart-Tilman, 4000 Liege, Belgium. yhenrotin@ulg.ac.be. FAU - de Val, Jose Eduardo Mate Sanchez AU - de Val JEMS AD - Department of Biomaterials Engineering, Universidad Catolica San Antonio de Murcia, 30107 Murcia, Spain. jemate@ucam.edu. FAU - M Regenstein, Joe AU - M Regenstein J AD - Department of Food Science, Cornell University, Ithaca, NY 14853-7201, USA. jmr9@cornell.edu. FAU - Lim, Sun Young AU - Lim SY AD - Division of Marine Bioscience, Korea Maritime and Ocean University, Busan 606791, Korea. sylim@kmou.ac.kr. FAU - Bao, Bin AU - Bao B AD - Department of Marine Bio-Pharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China. bbao@shou.edu.cn. FAU - Wu, Wenhui AU - Wu W AD - Department of Marine Bio-Pharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China. whwu@shou.edu.cn. AD - Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Huaihai Institute of Technology, Lianyungang 222005, China. whwu@shou.edu.cn. AD - Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation, Ministry of Agriculture, Shanghai 201306, China. whwu@shou.edu.cn. LA - eng PT - Journal Article DEP - 20180925 PL - Switzerland TA - Mar Drugs JT - Marine drugs JID - 101213729 RN - 0 (Collagen Type I) RN - EC 3.4.23.1 (Pepsin A) SB - IM MH - Animals MH - Bone and Bones/cytology/drug effects/*metabolism MH - Cell Differentiation MH - Cell Line MH - Cell Proliferation/*drug effects MH - Collagen Type I/chemistry/isolation & purification/*pharmacology/ultrastructure MH - Mesenchymal Stem Cells MH - Mice MH - Microscopy, Electron, Scanning MH - Osteoblasts MH - Osteogenesis/drug effects MH - Pepsin A/chemistry MH - *Sharks MH - Skin/chemistry MH - Solubility MH - Tissue Engineering/*methods MH - Viscosity PMC - PMC6212988 OTO - NOTNLM OT - Runx2 OT - blue shark collagen OT - differentiated mesenchymal stem cell OT - osteoblast OT - osteogenic activity OT - proliferation COIS- The authors declare no conflict of interest. EDAT- 2018/09/28 06:00 MHDA- 2019/01/12 06:00 PMCR- 2018/10/01 CRDT- 2018/09/28 06:00 PHST- 2018/08/22 00:00 [received] PHST- 2018/09/07 00:00 [revised] PHST- 2018/09/17 00:00 [accepted] PHST- 2018/09/28 06:00 [entrez] PHST- 2018/09/28 06:00 [pubmed] PHST- 2019/01/12 06:00 [medline] PHST- 2018/10/01 00:00 [pmc-release] AID - md16100350 [pii] AID - marinedrugs-16-00350 [pii] AID - 10.3390/md16100350 [doi] PST - epublish SO - Mar Drugs. 2018 Sep 25;16(10):350. doi: 10.3390/md16100350.