PMID- 30258898
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2352-3042 (Electronic)
IS  - 2352-4820 (Print)
IS  - 2352-3042 (Linking)
VI  - 3
IP  - 4
DP  - 2016 Dec
TI  - Differential alterations of positive and negative regulators of beta catenin 
      enhance endogenous expression and activity of beta catenin in A549 non small cell 
      lung cancer (NSCLC) cells.
PG  - 282-288
LID - 10.1016/j.gendis.2016.10.004 [doi]
AB  - Beta catenin has been well documented in previous studies to be involved in non 
      small cell lung cancer (NSCLC). Beta catenin abundance and transcriptional 
      activity are significantly regulated by several factors. Though it is well known 
      that Akt and Gsk3 beta are respective positive and negative regulators of beta 
      catenin, however, no single study has so far documented how the expression and 
      activity of both positive as well as negative regulators play favorable role on 
      beta catenin expression and activity in NSCLC. In this study, we compared 
      expression and activity of beta catenin and its regulators in normal lung cell 
      WI38 and NSCLC cell A549 by western blot, qRT-PCR and luciferase assay. We 
      observed that beta catenin positive regulators (Akt and Hsp90) and negative 
      regulators (Gsk3 beta and microRNA-214) have differential expression and/or 
      activity in NSCLC cell A549. However the differentially altered statuses of both 
      the positive and negative regulators rendered cumulative positive effect on beta 
      catenin expression and activity in A549. Our study thus suggests that 
      chemotherapeutic modulations of regulating factors are crucial when abrogation 
      and/or inhibition of key oncogenic proteins are necessary for cancer 
      chemotherapy.
FAU - Ghatak, Supratim
AU  - Ghatak S
AD  - Crystallography and Molecular Biology Division, Saha Institute of Nuclear 
      Physics, 1/AF Bidhannagar, Kolkata 700064, India.
FAU - Raha, Sanghamitra
AU  - Raha S
AD  - Department of Biotechnology and ISERC, Visva Bharati University, Santiniketan, 
      731235, India.
LA  - eng
PT  - Journal Article
DEP - 20161104
PL  - Netherlands
TA  - Genes Dis
JT  - Genes & diseases
JID - 101635967
PMC - PMC6147136
OTO - NOTNLM
OT  - A549
OT  - Beta catenin
OT  - Gsk3 beta
OT  - NSCLC, non small cell lung cancer
OT  - Non small cell lung cancer
OT  - SCLC, small cell lung cancer
OT  - miR-214
OT  - miRNA, microRNA
OT  - snRNA, small nuclear RNA
EDAT- 2016/11/04 00:00
MHDA- 2016/11/04 00:01
PMCR- 2016/11/04
CRDT- 2018/09/28 06:00
PHST- 2016/08/14 00:00 [received]
PHST- 2016/10/28 00:00 [accepted]
PHST- 2018/09/28 06:00 [entrez]
PHST- 2016/11/04 00:00 [pubmed]
PHST- 2016/11/04 00:01 [medline]
PHST- 2016/11/04 00:00 [pmc-release]
AID - S2352-3042(16)30044-7 [pii]
AID - 10.1016/j.gendis.2016.10.004 [doi]
PST - epublish
SO  - Genes Dis. 2016 Nov 4;3(4):282-288. doi: 10.1016/j.gendis.2016.10.004. 
      eCollection 2016 Dec.