PMID- 30260385
OWN - NLM
STAT- MEDLINE
DCOM- 20190311
LR  - 20211204
IS  - 1745-7270 (Electronic)
IS  - 1672-9145 (Linking)
VI  - 50
IP  - 11
DP  - 2018 Nov 1
TI  - Organosulfur compounds induce cytoprotective autophagy against apoptosis by 
      inhibiting mTOR phosphorylation activity in macrophages.
PG  - 1085-1093
LID - 10.1093/abbs/gmy114 [doi]
AB  - Organosulfur compounds (OSCs) are the bioactive components of garlic. Some OSCs 
      have apoptotic or autophagy-inducing effects. Autophagy plays roles in both 
      cytoprotection and apoptosis-related cell death, and the interaction between 
      autophagy and apoptosis is important in the modulation of immune responses. The 
      mechanism of an OSC-mediated effect via the interaction of autophagy and 
      apoptosis is unknown. In this study, the effects of five OSC compounds on 
      autophagy in the macrophage cell line RAW264.7 and primary macrophages were 
      investigated. We found that S-allylcysteine (SAC), diallyl disulde (DADS) and 
      diallyl tetrasulfide (DTS) treatment increased the number of autophagosomes of 
      RAW264.7 cells, inhibited the phosphorylation of ribosomal protein S6 kinase 
      beta-1 (p70S6K/S6K1) which is a substrate of mammalian target of rapamycin 
      (mTOR), and significantly enhanced autophagy flux. The induction of autophagy by 
      SAC, DADS and DTS was inhibited by stably knocking down the expression of 
      autophagy-related gene 5 (ATG5) with short hairpin RNA (shRNA). Further 
      experiments confirmed that SAC, DADS and DTS also induced apoptosis in RAW264.7 
      cells. The induction of apoptosis and Caspase 3 activity by SAC, DADS and DTS 
      were increased by stably knocking down of ATG5 expression with shRNA in RAW264.7 
      cells or treating with 5 mM 3-MA in primary macrophages. Our results suggest that 
      SAC, DADS and DTS induce both autophagy and apoptosis. The autophagy induction 
      protects macrophages from apoptosis by inhibiting mTOR phosphorylation activity 
      to maintain the mass of immune cells.
FAU - Wu, Yanyang
AU  - Wu Y
AD  - College of Food Science and Technology, Hunan Agricultural University, Changsha, 
      China.
FAU - Hu, Yongquan
AU  - Hu Y
AD  - Horticulture and Landscape College, Hunan Agricultural University, Changsha, 
      China.
AD  - Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, 
      Changsha, China.
AD  - State Key Laboratory of Subhealth Intervention Technology, Changsha, China.
FAU - Zhou, Haiyan
AU  - Zhou H
AD  - Horticulture and Landscape College, Hunan Agricultural University, Changsha, 
      China.
AD  - Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, 
      Changsha, China.
AD  - State Key Laboratory of Subhealth Intervention Technology, Changsha, China.
FAU - Zhu, Jiayu
AU  - Zhu J
AD  - Horticulture and Landscape College, Hunan Agricultural University, Changsha, 
      China.
AD  - Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, 
      Changsha, China.
AD  - State Key Laboratory of Subhealth Intervention Technology, Changsha, China.
FAU - Tong, Zhongyi
AU  - Tong Z
AD  - Department of Pathology, the Second Xiangya Hospital of Central South University, 
      Changsha, China.
FAU - Qin, Si
AU  - Qin S
AD  - College of Food Science and Technology, Hunan Agricultural University, Changsha, 
      China.
FAU - Liu, Dongbo
AU  - Liu D
AD  - Horticulture and Landscape College, Hunan Agricultural University, Changsha, 
      China.
AD  - Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, 
      Changsha, China.
AD  - State Key Laboratory of Subhealth Intervention Technology, Changsha, China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Acta Biochim Biophys Sin (Shanghai)
JT  - Acta biochimica et biophysica Sinica
JID - 101206716
RN  - 0 (Allyl Compounds)
RN  - 0 (Disulfides)
RN  - 0 (Organic Chemicals)
RN  - 0 (Protective Agents)
RN  - 0 (Sulfides)
RN  - 0 (Sulfur Compounds)
RN  - 5HI47O6OA7 (diallyl disulfide)
RN  - 81R3X99M15 (S-allylcysteine)
RN  - EC 2.7.1.1 (mTOR protein, mouse)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - K848JZ4886 (Cysteine)
RN  - WRP12IW1D7 (diallyl tetrasulfide)
SB  - IM
MH  - Allyl Compounds/pharmacology
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Autophagosomes/drug effects/metabolism
MH  - Autophagy/*drug effects
MH  - Cells, Cultured
MH  - Cysteine/analogs & derivatives/pharmacology
MH  - Disulfides/pharmacology
MH  - Garlic/chemistry
MH  - Macrophages/cytology/*drug effects/metabolism
MH  - Mice
MH  - Organic Chemicals/*pharmacology
MH  - Phosphorylation/drug effects
MH  - Protective Agents/pharmacology
MH  - RAW 264.7 Cells
MH  - Sulfides/pharmacology
MH  - Sulfur Compounds/*pharmacology
MH  - TOR Serine-Threonine Kinases/*metabolism
EDAT- 2018/09/28 06:00
MHDA- 2019/03/12 06:00
CRDT- 2018/09/28 06:00
PHST- 2018/03/13 00:00 [received]
PHST- 2018/09/28 06:00 [pubmed]
PHST- 2019/03/12 06:00 [medline]
PHST- 2018/09/28 06:00 [entrez]
AID - 5107347 [pii]
AID - 10.1093/abbs/gmy114 [doi]
PST - ppublish
SO  - Acta Biochim Biophys Sin (Shanghai). 2018 Nov 1;50(11):1085-1093. doi: 
      10.1093/abbs/gmy114.