PMID- 30260397
OWN - NLM
STAT- MEDLINE
DCOM- 20200128
LR  - 20200128
IS  - 1460-2709 (Electronic)
IS  - 1369-3786 (Linking)
VI  - 57
IP  - 7
DP  - 2019 Oct 1
TI  - Intradermal injection of Pythium insidiosum protein antigens for improved 
      diagnosis and treatment of pythiosis in an experimental model.
PG  - 807-812
LID - 10.1093/mmy/myy078 [doi]
AB  - The oomycetous pathogen Pythium insidiosum is the causative agent of pythiosis, a 
      life-threatening disease that affects animals and humans. This infectious disease 
      is difficult to treat, and early and accurate diagnosis is critical for effective 
      treatment. In this sense, this study aimed to evaluate the intradermal (ID) 
      injection of P. insidiosum protein antigens (PiPA) for the diagnosis and 
      treatment of pythiosis using an experimental model. For diagnostic purposes, PiPA 
      were injected by the ID route in the following groups of rabbits: (a) control; 
      (b) previously immunized with PiPA injected by the subcutaneous (SC) route; and 
      (c) infected with P. insidiosum zoospores. For treatment purposes, rabbits with 
      pythiosis were also treated with PiPA by the ID or SC routes. Mean induration 
      sizes were different at 24 h and 72 h readings when compared to the control 
      group. Sensitivity of the protocol was 100% at 24 h and 80% at 72 h, with 100% 
      specificity in both readings. PiPA treatment using ID or SC routes did not result 
      in significant differences in lesion sizes and cure rates; however, serum levels 
      of interferon-gamma were higher in SC route. This study demonstrates the 
      applicability of PiPA ID for diagnosis and treatment of pythiosis in an 
      experimental model.
CI  - (c) The Author(s) 2018. Published by Oxford University Press on behalf of The 
      International Society for Human and Animal Mycology.
FAU - Weiblen, Carla
AU  - Weiblen C
AD  - Universidade Federal de Santa Maria (UFSM). aDepartamento de Medicina Veterinaria 
      Preventiva (DMVP)/ Programa de Pos Graduacao em Medicina Veterinaria (PPGMV)/ 
      Centro de Ciencias Rurais (CCR). bDepartamento de Microbiologia e Parasitologia 
      (Demip)/Centro de Ciencias da Saude (CCS).
FAU - Zanette, Regis Adriel
AU  - Zanette RA
AD  - Universidade Federal do Rio Grande do Sul (UFRGS), Programa de Pos-Graduacao em 
      Ciencias Biologicas: Farmacologia e Terapeutica.
FAU - Ribeiro, Tatiana Correa
AU  - Ribeiro TC
AD  - Universidade Federal de Santa Maria (UFSM). aDepartamento de Medicina Veterinaria 
      Preventiva (DMVP)/ Programa de Pos Graduacao em Medicina Veterinaria (PPGMV)/ 
      Centro de Ciencias Rurais (CCR). bDepartamento de Microbiologia e Parasitologia 
      (Demip)/Centro de Ciencias da Saude (CCS).
FAU - Pereira Dos Santos, Carlos Eduardo
AU  - Pereira Dos Santos CE
AD  - Universidade Federal do Mato Grosso (UFMT).
FAU - Ianiski, Lara Baccarin
AU  - Ianiski LB
AD  - Universidade Federal de Santa Maria (UFSM). aDepartamento de Medicina Veterinaria 
      Preventiva (DMVP)/ Programa de Pos Graduacao em Medicina Veterinaria (PPGMV)/ 
      Centro de Ciencias Rurais (CCR). bDepartamento de Microbiologia e Parasitologia 
      (Demip)/Centro de Ciencias da Saude (CCS).
FAU - Pereira, Daniela Isabel Brayer
AU  - Pereira DIB
AD  - Universidade Federal de Pelotas (UFPel). Instituto de Biologia (IB), Departamento 
      de Microbiologia e Parasitologia.
FAU - Santurio, Janio Morais
AU  - Santurio JM
AD  - Universidade Federal de Santa Maria (UFSM). aDepartamento de Medicina Veterinaria 
      Preventiva (DMVP)/ Programa de Pos Graduacao em Medicina Veterinaria (PPGMV)/ 
      Centro de Ciencias Rurais (CCR). bDepartamento de Microbiologia e Parasitologia 
      (Demip)/Centro de Ciencias da Saude (CCS).
FAU - Botton, Sonia de Avila
AU  - Botton SA
AD  - Universidade Federal de Santa Maria (UFSM). aDepartamento de Medicina Veterinaria 
      Preventiva (DMVP)/ Programa de Pos Graduacao em Medicina Veterinaria (PPGMV)/ 
      Centro de Ciencias Rurais (CCR). bDepartamento de Microbiologia e Parasitologia 
      (Demip)/Centro de Ciencias da Saude (CCS).
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - England
TA  - Med Mycol
JT  - Medical mycology
JID - 9815835
RN  - 0 (Antigens)
RN  - 0 (Cytokines)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antigens/*administration & dosage/immunology
MH  - Cytokines/blood
MH  - Disease Models, Animal
MH  - Injections, Intradermal
MH  - Interferon-gamma/blood
MH  - Pythiosis/*diagnosis/*therapy
MH  - Pythium/*chemistry/immunology
MH  - Rabbits
OTO - NOTNLM
OT  - Pythiosis
OT  - cytokines
OT  - intradermal route
OT  - rabbits
EDAT- 2018/09/28 06:00
MHDA- 2020/01/29 06:00
CRDT- 2018/09/28 06:00
PHST- 2018/05/15 00:00 [received]
PHST- 2018/08/08 00:00 [revised]
PHST- 2018/08/27 00:00 [accepted]
PHST- 2018/09/28 06:00 [pubmed]
PHST- 2020/01/29 06:00 [medline]
PHST- 2018/09/28 06:00 [entrez]
AID - 5107545 [pii]
AID - 10.1093/mmy/myy078 [doi]
PST - ppublish
SO  - Med Mycol. 2019 Oct 1;57(7):807-812. doi: 10.1093/mmy/myy078.