PMID- 30260554
OWN - NLM
STAT- MEDLINE
DCOM- 20191024
LR  - 20191024
IS  - 1552-4965 (Electronic)
IS  - 1549-3296 (Linking)
VI  - 106
IP  - 12
DP  - 2018 Dec
TI  - Xenogeneic acellular nerve scaffolds supplemented with autologous bone 
      marrow-derived stem cells promote axonal outgrowth and remyelination but not 
      nerve function.
PG  - 3065-3078
LID - 10.1002/jbm.a.36497 [doi]
AB  - Autologous nerves, artificial scaffolds or acellular nerve scaffolds are commonly 
      used in bridging treatment for peripheral nerve defects. Xenogeneic acellular 
      nerve scaffolds and allogeneic cellular nerve scaffolds have the same structural 
      characteristics. Due to the wider source of raw materials, these latter scaffolds 
      have high-potential value for applications. However, whether their heterogeneity 
      will affect nerve regeneration is unknown. The current study evaluated the 
      efficiency of xenogeneic acellular nerve scaffolds (XANs) combined with 
      5-ethynyl-2'-deoxyuridine (EdU)-labeling of autologous bone marrow-derived stem 
      cells (BMSCs) for repair of a 1.5 cm gap in rat sciatic nerves. XANs from rabbit 
      tibial nerves were prepared, the structure and components of the scaffolds were 
      evaluated after completely removing the cellular components. Animals were divided 
      into four groups based on graft: the simple XAN group, the XAN + BMSC group, the 
      XAN + Media (from BMSC culture) group, and the autograft group. Serological 
      immune tests showed that XANs induce an immune response in the first 2 weeks 
      after transplantation. Moreover, cell tracking revealed that the proportion of 
      EdU+ cells decreased over time, as shown by the measures at 2 days (70%), 4 days 
      (20%), and 8 days (even <3%) postoperatively. Nerve functional analyses revealed 
      that in contrast to the autograft group results, the XAN-BMSC, XAN + Media, and 
      XAN groups did not exhibit good restoration of the sciatic functional index (SFI) 
      or electrophysiological results (the peak action potential amplitudes) 12 weeks, 
      postoperatively. However, the XAN-BMSC and autograft groups demonstrated greater 
      remyelination and increased axon numbers and myelin thickness than the XAN + 
      Media and XAN groups 12 weeks, postoperatively (p < .05). In conclusion, in the 
      early stage of transplantation, XANs induce a certain degree of inflammation. 
      Although the combination of XANs with autologous BMSCs enhanced the number of 
      regenerated axons and the remyelination, the combination did not effectively 
      improve the recovery of nervous motor function. (c) 2018 Wiley Periodicals, Inc. J 
      Biomed Mater Res Part A: 106A: 3065-3078, 2018.
CI  - (c) 2018 Wiley Periodicals, Inc.
FAU - Hou, Bo
AU  - Hou B
AD  - Departments of Neurosurgery, The Third Affiliated Hospital, Sun Yat-Sen 
      University, Guangdong Province, 510630, Guangzhou, China.
FAU - Cai, Meiqin
AU  - Cai M
AD  - Departments of Neurosurgery, The Third Affiliated Hospital, Sun Yat-Sen 
      University, Guangdong Province, 510630, Guangzhou, China.
FAU - Chen, Chuan
AU  - Chen C
AD  - Departments of Neurosurgery, The Third Affiliated Hospital, Sun Yat-Sen 
      University, Guangdong Province, 510630, Guangzhou, China.
FAU - Ji, Wanqing
AU  - Ji W
AD  - Department of Obstetrics, Guangzhou Women and Children's Medical Center, 
      Guangzhou Medical University, Guangdong Province, 510623, Guangzhou, China.
FAU - Ye, Zhuopeng
AU  - Ye Z
AD  - Departments of Neurosurgery, The Third Affiliated Hospital, Sun Yat-Sen 
      University, Guangdong Province, 510630, Guangzhou, China.
FAU - Ling, Cong
AU  - Ling C
AD  - Departments of Neurosurgery, The Third Affiliated Hospital, Sun Yat-Sen 
      University, Guangdong Province, 510630, Guangzhou, China.
FAU - Chen, Zhuopeng
AU  - Chen Z
AD  - Departments of Neurosurgery, The Third Affiliated Hospital, Sun Yat-Sen 
      University, Guangdong Province, 510630, Guangzhou, China.
FAU - Guo, Ying
AU  - Guo Y
AUID- ORCID: 0000-0001-8797-9052
AD  - Departments of Neurosurgery, The Third Affiliated Hospital, Sun Yat-Sen 
      University, Guangdong Province, 510630, Guangzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180907
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation/methods
MH  - Mesenchymal Stem Cells/*cytology
MH  - *Nerve Regeneration
MH  - *Neuronal Outgrowth
MH  - Rabbits
MH  - Rats, Sprague-Dawley
MH  - *Remyelination
MH  - Tissue Scaffolds/*chemistry
MH  - Transplantation, Autologous/methods
OTO - NOTNLM
OT  - bone marrow-derived stem cells
OT  - immune inflammation
OT  - motor function
OT  - nerve regeneration
OT  - nerve scaffold
OT  - xenogeneic
EDAT- 2018/09/28 06:00
MHDA- 2019/10/28 06:00
CRDT- 2018/09/28 06:00
PHST- 2018/02/26 00:00 [received]
PHST- 2018/06/07 00:00 [revised]
PHST- 2018/06/27 00:00 [accepted]
PHST- 2018/09/28 06:00 [pubmed]
PHST- 2019/10/28 06:00 [medline]
PHST- 2018/09/28 06:00 [entrez]
AID - 10.1002/jbm.a.36497 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2018 Dec;106(12):3065-3078. doi: 10.1002/jbm.a.36497. Epub 
      2018 Sep 7.