PMID- 30261345
OWN - NLM
STAT- MEDLINE
DCOM- 20190322
LR  - 20190322
IS  - 1873-4367 (Electronic)
IS  - 0927-7765 (Linking)
VI  - 173
DP  - 2019 Jan 1
TI  - Surface functionalization of halloysite nanotubes with supermagnetic iron oxide, 
      chitosan and 2-D calcium-phosphate nanoflakes for synergistic osteoconduction 
      enhancement of human adipose tissue-derived mesenchymal stem cells.
PG  - 18-26
LID - S0927-7765(18)30656-8 [pii]
LID - 10.1016/j.colsurfb.2018.09.045 [doi]
AB  - Halloysite nanotubes (HNTs) are known to be the highly emerging materials in 
      nano-medicinal applications. However, comprehensive exploitation of HNTs for the 
      regenerative medicinal applications is still necessary to be done. Therefore, 
      towards enhancing the osteogenic potential of human adipose tissue-derived 
      mesenchymal stem cells (hADMSCs), this study synthesized a novel and 
      multifunctional nanoscaffold of chitosan (CTs) functionalized supermagnetic 
      halloysite nanotubes (M-HNTs) decorated with the calcium phosphate 2-D nanoflakes 
      (CaP) (termed as; M-HNTs-CTs-CaP). Stepwise modified nanoscaffolds were 
      characterized by FE-SEM, FE-SEM-EDS, FE-HR-TEM, XPS, FT-IR and VSM analyses. The 
      hADMSCs osteogenic potential was confirmed by calcification (Alizarin Red S 
      staining), phosphate quantification and immunocytochemistry. Nanoscaffolds; CaP, 
      M-HNTs-CaP and M-HNTs-CTs-CaP were significantly enhanced and up-regulated 
      osteogenic potential compared to the HNTs, M-HNTs, M-HNTs-CTs. Among the 
      nanoscaffolds studied, M-HNTs-CTs-CaP exhibited highest osteogenesis, due to the 
      enhanced CaP distribution on M-HNTs-CTs surface, and synergistic osteoconduction 
      contributed from Fe(3)O(4), chitosan and CaP. Moreover, immunocytochemistry 
      analysis and morphologically observation showed well differentiated osteoblast on 
      the M-HNTs-CTs-CaP surface. Therefore, M-HNTs-CTs-CaP found to have a strong 
      osteogenic potential of hADMSCs, and might be serve as highly applicable, next 
      generation nanoscaffold for bone tissue engineering application.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Lee, Yoo-Jung
AU  - Lee YJ
AD  - Department of Life Sciences, Dongguk University-Seoul, Biomedi Campus, 32 
      Dongguk-ro, Ilsandong-gu, Goyang-si, Gyeonggi-do, 10326, Republic of Korea.
FAU - Lee, Seung-Cheol
AU  - Lee SC
AD  - Department of Life Sciences, Dongguk University-Seoul, Biomedi Campus, 32 
      Dongguk-ro, Ilsandong-gu, Goyang-si, Gyeonggi-do, 10326, Republic of Korea.
FAU - Jee, Seung Cheol
AU  - Jee SC
AD  - Department of Life Sciences, Dongguk University-Seoul, Biomedi Campus, 32 
      Dongguk-ro, Ilsandong-gu, Goyang-si, Gyeonggi-do, 10326, Republic of Korea.
FAU - Sung, Jung-Suk
AU  - Sung JS
AD  - Department of Life Sciences, Dongguk University-Seoul, Biomedi Campus, 32 
      Dongguk-ro, Ilsandong-gu, Goyang-si, Gyeonggi-do, 10326, Republic of Korea.
FAU - Kadam, Avinash A
AU  - Kadam AA
AD  - Research Institute of Biotechnology and Medical Converged Science, Dongguk 
      University-Seoul, Biomedi Campus, Ilsandong-gu, Goyang-si, Gyeonggi-do, 10326, 
      Republic of Korea. Electronic address: kadamavinash@dongguk.edu.
LA  - eng
PT  - Journal Article
DEP - 20180920
PL  - Netherlands
TA  - Colloids Surf B Biointerfaces
JT  - Colloids and surfaces. B, Biointerfaces
JID - 9315133
RN  - 0 (Calcium Phosphates)
RN  - 0 (Magnetite Nanoparticles)
RN  - 9012-76-4 (Chitosan)
RN  - 97Z1WI3NDX (calcium phosphate)
RN  - T1FAD4SS2M (Clay)
SB  - IM
MH  - Adipose Tissue/*cytology/physiology
MH  - Animals
MH  - Bone Regeneration/physiology
MH  - Bone and Bones/cytology/physiology
MH  - Calcification, Physiologic
MH  - Calcium Phosphates/*chemistry
MH  - Cell Differentiation
MH  - Chitosan/chemistry
MH  - Clay/*chemistry
MH  - Humans
MH  - Magnetite Nanoparticles/chemistry/ultrastructure
MH  - Mesenchymal Stem Cells/cytology/physiology
MH  - Nanotubes/*chemistry/ultrastructure
MH  - Osteoblasts/*cytology/physiology
MH  - Surface Properties
MH  - Tissue Engineering
MH  - *Tissue Scaffolds
OTO - NOTNLM
OT  - Bone tissue engineering
OT  - Calcium phosphate nanoflakes
OT  - Halloysite nanotubes
OT  - Multifunctional nanoscaffolds
OT  - Osteoconduction
OT  - hADMSCs
EDAT- 2018/09/28 06:00
MHDA- 2019/03/23 06:00
CRDT- 2018/09/28 06:00
PHST- 2018/02/06 00:00 [received]
PHST- 2018/08/19 00:00 [revised]
PHST- 2018/09/19 00:00 [accepted]
PHST- 2018/09/28 06:00 [pubmed]
PHST- 2019/03/23 06:00 [medline]
PHST- 2018/09/28 06:00 [entrez]
AID - S0927-7765(18)30656-8 [pii]
AID - 10.1016/j.colsurfb.2018.09.045 [doi]
PST - ppublish
SO  - Colloids Surf B Biointerfaces. 2019 Jan 1;173:18-26. doi: 
      10.1016/j.colsurfb.2018.09.045. Epub 2018 Sep 20.