PMID- 30261503
OWN - NLM
STAT- MEDLINE
DCOM- 20181023
LR  - 20190212
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 49
IP  - 6
DP  - 2018
TI  - The Long Noncoding RNA TUG1 Promotes Laryngeal Cancer Proliferation and 
      Migration.
PG  - 2511-2520
LID - 10.1159/000493876 [doi]
AB  - BACKGROUND/AIMS: Researchers have shown that long noncoding RNAs are closely 
      associated with the pathogenesis of laryngeal squamous cell carcinoma (LSCC). 
      However, the role of the long noncoding RNA taurine-upregulated gene 1 (TUG1) in 
      the pathogenesis of LSCC remains unclear, although it is recognized as an 
      oncogenic regulator for several types of squamous cell carcinoma. METHODS: 
      qRT-PCR was performed to measure the expression of TUG1 in LSCC tissues and cell 
      lines. 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) 
      was used to measure the effect of TUG1 on cell proliferation. Transwell assay and 
      flow cytometry were employed to determine the effect of TUG1 on cell migration 
      and invasion. Western-blot were performed to explore the relation of TUG1 and p53 
      mRNA. RESULTS: Higher TUG1 expression in LSCC than in paired normal 
      tumor-adjacent tissue specimens (N = 64) was observed using quantitative 
      real-time polymerase chain reaction. Also, high TUG1 expression was positively 
      associated with advanced T category, worse lymph node metastasis and late 
      clinical stage. Furthermore, in vitro experiments demonstrated that silencing of 
      TUG1 markedly inhibited proliferation, cell-cycle progression, migration, and 
      invasion of LSCC cells, whereas depletion of TUG1 led to increased apoptosis. 
      CONCLUSION: These findings demonstrated that upregulated TUG1 expression exerted 
      oncogenic effects by promoting proliferation, migration, and invasion, and 
      inhibiting apoptosis in LSCC cells.
CI  - (c) 2018 The Author(s). Published by S. Karger AG, Basel.
FAU - Zhang, Zhonghua
AU  - Zhang Z
AD  - Department of Otorhinolaryngology, Qilu Hospital, Shandong University, Key 
      Laboratory of Otolaryngology, NHFPC (Shandong University), Jinan, China.
FAU - Wang, Xuehai
AU  - Wang X
AD  - Department of Otorhinolaryngology, Weihai Municipal Hospital, Weihai, China.
FAU - Cao, Shengda
AU  - Cao S
AD  - Department of Otorhinolaryngology, Qilu Hospital, Shandong University, Key 
      Laboratory of Otolaryngology, NHFPC (Shandong University), Jinan, China.
FAU - Han, Xiao
AU  - Han X
AD  - Department of Otorhinolaryngology, Qilu Hospital, Shandong University, Key 
      Laboratory of Otolaryngology, NHFPC (Shandong University), Jinan, China.
FAU - Wang, Zhanwang
AU  - Wang Z
AD  - Department of Otorhinolaryngology, Qilu Hospital, Shandong University, Key 
      Laboratory of Otolaryngology, NHFPC (Shandong University), Jinan, China.
FAU - Zhao, Xiaoyan
AU  - Zhao X
AD  - Department of Otorhinolaryngology, Qilu Hospital, Shandong University, Key 
      Laboratory of Otolaryngology, NHFPC (Shandong University), Jinan, China.
FAU - Liu, Xuejun
AU  - Liu X
AD  - Department of Otorhinolaryngology, Qilu Hospital, Shandong University, Key 
      Laboratory of Otolaryngology, NHFPC (Shandong University), Jinan, China.
FAU - Li, Guojun
AU  - Li G
AD  - Department of Head and Neck Surgery, The University of Texas MD Anderson Cancer 
      Center, Houston, Texas, USA.
AD  - Department of Epidemiology, The University of Texas MD Anderson Cancer Center, 
      Houston, Texas, USA.
FAU - Pan, Xinliang
AU  - Pan X
AD  - Department of Otorhinolaryngology, Qilu Hospital, Shandong University, Key 
      Laboratory of Otolaryngology, NHFPC (Shandong University), Jinan, China.
FAU - Lei, Dapeng
AU  - Lei D
AD  - Department of Otorhinolaryngology, Qilu Hospital, Shandong University, Key 
      Laboratory of Otolaryngology, NHFPC (Shandong University), Jinan, China.
LA  - eng
PT  - Journal Article
DEP - 20180927
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (TP53 protein, human)
RN  - 0 (TUG1 long noncoding RNA, human)
RN  - 0 (Tumor Suppressor Protein p53)
SB  - IM
MH  - Carcinoma, Squamous Cell/genetics/*pathology
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - *Cell Proliferation
MH  - Female
MH  - Humans
MH  - Laryngeal Neoplasms/genetics/*pathology
MH  - Male
MH  - Middle Aged
MH  - RNA Interference
MH  - RNA, Long Noncoding/antagonists & inhibitors/genetics/*metabolism
MH  - RNA, Small Interfering/metabolism
MH  - S Phase Cell Cycle Checkpoints
MH  - Tumor Suppressor Protein p53/genetics/metabolism
OTO - NOTNLM
OT  - Laryngeal squamous cell carcinoma
OT  - Long noncoding RNAs
OT  - TUG1
OT  - metastasis
OT  - proliferation
EDAT- 2018/09/28 06:00
MHDA- 2018/10/24 06:00
CRDT- 2018/09/28 06:00
PHST- 2018/01/25 00:00 [received]
PHST- 2018/09/18 00:00 [accepted]
PHST- 2018/09/28 06:00 [pubmed]
PHST- 2018/10/24 06:00 [medline]
PHST- 2018/09/28 06:00 [entrez]
AID - 000493876 [pii]
AID - 10.1159/000493876 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2018;49(6):2511-2520. doi: 10.1159/000493876. Epub 2018 Sep 
      27.