PMID- 30261897
OWN - NLM
STAT- MEDLINE
DCOM- 20190116
LR  - 20190116
IS  - 1746-160X (Electronic)
IS  - 1746-160X (Linking)
VI  - 14
IP  - 1
DP  - 2018 Sep 27
TI  - Effect of DACH1 on proliferation and invasion of laryngeal squamous cell 
      carcinoma.
PG  - 20
LID - 10.1186/s13005-018-0177-1 [doi]
LID - 20
AB  - BACKGROUND: To investigate the effect of DACH1 over-expression on proliferation 
      and invasion of laryngeal squamous cell carcinoma (LSCC). METHODS: The 120 cases 
      of LSCC tumors and 114 adjacent non-neoplastic tissues were collected to detect 
      the expression of DACH1 by immunohistochemistry. The changes of DACH1 expression 
      from each group were assessed and correlated to the clinical parameters of the 
      patients. Plasmid-DACH1 was transfected into Hep-2 cells to up-regulate the 
      expression of DACH1C. Real-time PCR, Western blot, CCK8 and transwell assay were 
      used to verify the cell proliferation and invasion after plasmid-DACH1 
      transfection. RESULTS: The results indicated that DACH1 was downregulated in LSCC 
      tissues as compared to corresponding adjacent non-neoplastic tissues. Decreased 
      expression of DACH1 was found in the tumors upraglottic tumor, lymph node 
      metastases, T3-4 stage and advanced clinical stage. In Hep-2 cells, transfection 
      with plasmid-DACH1 could suppress cell proliferation, invasion and induce G1 
      phase extension in cell cycle. CONCLUSIONS: DACH1 may act as a tumor suppressor 
      gene and could be a potential target for therapeutic intervention of LSCC.
FAU - Zhang, Jiarui
AU  - Zhang J
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Second Affiliated 
      Hospital, Harbin Medical University, Harbin, 150081, China.
FAU - Ren, Xiuxia
AU  - Ren X
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Second Affiliated 
      Hospital, Harbin Medical University, Harbin, 150081, China.
FAU - Wang, Bo
AU  - Wang B
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Second Affiliated 
      Hospital, Harbin Medical University, Harbin, 150081, China.
FAU - Cao, Jing
AU  - Cao J
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Second Affiliated 
      Hospital, Harbin Medical University, Harbin, 150081, China.
FAU - Tian, Linli
AU  - Tian L
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Second Affiliated 
      Hospital, Harbin Medical University, Harbin, 150081, China. tianlinli78@163.com.
FAU - Liu, Ming
AU  - Liu M
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Second Affiliated 
      Hospital, Harbin Medical University, Harbin, 150081, China. 
      liuming002@outlook.com.
LA  - eng
GR  - 81572647 and 81372902/National Natural Science Foundation of China/
GR  - 81402234/National Natural Science Foundation of China/
GR  - (LBH-Q16157/postdoctoral scientific research developmental fund/
PT  - Comparative Study
PT  - Journal Article
DEP - 20180927
PL  - England
TA  - Head Face Med
JT  - Head & face medicine
JID - 101245792
RN  - 0 (DACH1 protein, human)
RN  - 0 (Eye Proteins)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Aged
MH  - Biopsy, Needle
MH  - Blotting, Western
MH  - Carcinoma, Squamous Cell/*genetics/pathology/surgery
MH  - Case-Control Studies
MH  - Cell Proliferation/*genetics
MH  - Eye Proteins/*genetics
MH  - Female
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Immunohistochemistry
MH  - Laryngeal Neoplasms/*genetics/pathology/surgery
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Invasiveness/pathology
MH  - Neoplasm Staging
MH  - Prognosis
MH  - Real-Time Polymerase Chain Reaction
MH  - Retrospective Studies
MH  - Transcription Factors/*genetics
MH  - Up-Regulation
PMC - PMC6161397
OTO - NOTNLM
OT  - DACH1
OT  - Invasion
OT  - LSCC
OT  - Proliferation
COIS- AUTHORS' INFORMATION: Linli Tian is a professor of ENT at the Harbin Medical 
      University, who serves at the second affiliated hospital from 2003 to now. She 
      specializes in working with the laryngopharyngeal diseases. ETHICS APPROVAL AND 
      CONSENT TO PARTICIPATE: The authors assert that all procedures contributing to 
      this work comply with the ethical standards of the relevant national and 
      institutional guidelines on human experimentation (the ethic committee of the 
      Second Affiliated Hospital, Harbin Medical University). The number is KY2017-009. 
      All of patients are agree to publish their informations. CONSENT FOR PUBLICATION: 
      All patients have consent to publish. The authors all consent for publition. 
      COMPETING INTERESTS: The authors declare that they have no competing interests. 
      PUBLISHER'S NOTE: Springer Nature remains neutral with regard to jurisdictional 
      claims in published maps and institutional affiliations.
EDAT- 2018/09/29 06:00
MHDA- 2019/01/17 06:00
PMCR- 2018/09/27
CRDT- 2018/09/29 06:00
PHST- 2017/09/11 00:00 [received]
PHST- 2018/09/19 00:00 [accepted]
PHST- 2018/09/29 06:00 [entrez]
PHST- 2018/09/29 06:00 [pubmed]
PHST- 2019/01/17 06:00 [medline]
PHST- 2018/09/27 00:00 [pmc-release]
AID - 10.1186/s13005-018-0177-1 [pii]
AID - 177 [pii]
AID - 10.1186/s13005-018-0177-1 [doi]
PST - epublish
SO  - Head Face Med. 2018 Sep 27;14(1):20. doi: 10.1186/s13005-018-0177-1.