PMID- 30264469
OWN - NLM
STAT- MEDLINE
DCOM- 20190214
LR  - 20190215
IS  - 1098-2280 (Electronic)
IS  - 0893-6692 (Linking)
VI  - 60
IP  - 1
DP  - 2019 Jan
TI  - Chromosome Missegregation and Aneuploidy Induction in Human Peripheral Blood 
      Lymphocytes In vitro by Low Concentrations of Chlorpyrifos, Imidacloprid and 
      alpha-Cypermethrin.
PG  - 72-84
LID - 10.1002/em.22235 [doi]
AB  - Chlorpyrifos, imidacloprid, and alpha-cypermethrin are some of the most widely used 
      insecticides in contemporary agriculture. However, their low-dose, nontarget 
      genotoxic effects have not been extensively assayed. As one of the most relevant 
      cancer biomarkers, we aimed to assess the aneuploidy due to chromosome 
      missegregation during mitosis. To aim it we treated human lymphocytes in vitro 
      with three concentrations of insecticides equivalents relevant for real scenario 
      exposure assessed by regulatory agencies. We focused on chlorpyrifos as 
      conventional and imidacloprid and alpha-cypermethrin as sustainable use insecticides. 
      Cytokinesis-blocked micronucleus assay was performed coupled with fluorescence in 
      situ hybridization (FISH) with directly labeled pancentromeric probes for 
      chromosomes 9, 18, X and Y. None of the insecticides induced significant 
      secondary DNA damage in terms of micronuclei (MN), nuclear buds (NB), or 
      nucleoplasmic bridges (NPB). However, significant disbalances in chromosomes 9, 
      18, X and Y, and in insecticide-treated cells has been observed. According to 
      recent studies, these disbalances in chromosome numbers may be atributted to 
      defect sister chromatid cohesion which contribute to the increase of chromosome 
      missegregation but not to micronuclei incidence. We conclude that tested 
      insecticidal active substances exert chromosome missegregation effects at low 
      concentrations, possibly by mechanism of sister chromatid cohesion. These 
      findings may contribute to future risk assesments and understanding of 
      insecticide mode of action on human genome. Environ. Mol. Mutagen. 60:72-84, 
      2019. (c) 2018 Wiley Periodicals, Inc.
CI  - (c) 2018 Wiley Periodicals, Inc.
FAU - Muzinic, Vedran
AU  - Muzinic V
AD  - Unit of Mutagenesis, Institute for Medical Research and Occupational Health, 
      Zagreb, Croatia.
FAU - Ramic, Snjezana
AU  - Ramic S
AD  - Department of Oncological Pathology, Sestre Milosrdnice University Hospital 
      Center, Zagreb, Croatia.
FAU - Zeljezic, Davor
AU  - Zeljezic D
AD  - Unit of Mutagenesis, Institute for Medical Research and Occupational Health, 
      Zagreb, Croatia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180927
PL  - United States
TA  - Environ Mol Mutagen
JT  - Environmental and molecular mutagenesis
JID - 8800109
RN  - 0 (Insecticides)
RN  - 0 (Neonicotinoids)
RN  - 0 (Nitro Compounds)
RN  - 0 (Pyrethrins)
RN  - 1TR49121NP (cypermethrin)
RN  - 3BN7M937V8 (imidacloprid)
RN  - JCS58I644W (Chlorpyrifos)
SB  - IM
MH  - *Aneuploidy
MH  - Chlorpyrifos/*toxicity
MH  - Chromosome Aberrations/*chemically induced
MH  - Chromosome Segregation/*drug effects/genetics
MH  - Chromosomes, Human, Pair 18/drug effects
MH  - Chromosomes, Human, Pair 9/drug effects
MH  - Chromosomes, Human, X/drug effects
MH  - Chromosomes, Human, Y/drug effects
MH  - DNA Damage/*drug effects
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Insecticides/*toxicity
MH  - Lymphocytes/drug effects
MH  - Micronucleus Tests
MH  - Neonicotinoids/*toxicity
MH  - Nitro Compounds/*toxicity
MH  - Pyrethrins/*toxicity
OTO - NOTNLM
OT  - Chlorpyrifos
OT  - aneuploidy
OT  - fluorescence in situ hybridization
OT  - genotoxicity
OT  - imidacloprid
OT  - alpha-cypermethrin
EDAT- 2018/09/29 06:00
MHDA- 2019/02/15 06:00
CRDT- 2018/09/29 06:00
PHST- 2018/04/04 00:00 [received]
PHST- 2018/07/05 00:00 [revised]
PHST- 2018/07/12 00:00 [accepted]
PHST- 2018/09/29 06:00 [pubmed]
PHST- 2019/02/15 06:00 [medline]
PHST- 2018/09/29 06:00 [entrez]
AID - 10.1002/em.22235 [doi]
PST - ppublish
SO  - Environ Mol Mutagen. 2019 Jan;60(1):72-84. doi: 10.1002/em.22235. Epub 2018 Sep 
      27.