PMID- 30265371 OWN - NLM STAT- MEDLINE DCOM- 20190311 LR - 20211204 IS - 1525-3171 (Electronic) IS - 0032-5791 (Linking) VI - 98 IP - 3 DP - 2019 Mar 1 TI - Effects of in ovo feeding of L-leucine on amino acids metabolism and heat-shock protein-70, and -90 mRNA expression in heat-exposed chicks. PG - 1243-1253 LID - 10.3382/ps/pey444 [doi] AB - Recently, we found that in ovo feeding of L-leucine (L-Leu) stimulated the metabolism of lipids and afforded thermotolerance in male Chunky broiler chicks. In this study, we investigated the effects of feeding L-Leu in ovo on the metabolism of amino acids and on the cellular stress response mainly in the central and peripheral tissues in neonatal male broiler chicks and partly in embryonic tissues. Chicks (9 d old) were exposed to high ambient temperature (HT: 35 +/- 1 degrees C) or control thermoneutral temperature (CT: 28 +/- 1 degrees C) for 180 min. The ambient temperatures were based on our recent reports and the recommendation of the Chunky broiler manual in which 28 degrees C has been suggested as a normal ambient temperature for 5 to 9-d-old broiler chicks. In ovo feeding of L-Leu caused a significant (P < 0.05) decline in diencephalic arginine concentrations but it increased the diencephalic and plasma lysine concentrations when compared with the control chicks under HT. Notably, in ovo feeding of L-Leu significantly (P < 0.05) attenuated the increment of hepatic arginine compared with the control chicks under HT. Interestingly, in ovo feeding of L-Leu significantly (P < 0.05) attenuated the diencephalic gene expression of heat-shock protein (HSP) -70 and -90 in heat-exposed chicks. The gene expressions of mammalian target of rapamycin (mTOR) and its downstream genes (ribosomal protein S6 kinase (S6K1) and eukaryotic initiation factor 4E binding protein 1 (4E-BP1)) in the central and peripheral tissues were not influenced in the chicks under heat stress. We found that the gene expressions of mTOR, S6K1, and 4E-BP1 were significantly (P < 0.05) stimulated only in the embryonic breast muscle, and not in the other embryonic tissues, by in ovo feeding of L-Leu. In conclusion, in ovo feeding of L-Leu caused a change in the metabolism of amino acids in response to heat stress in broiler chicks. Attenuated gene expressions of HSP-70 and -90 under heat stress further suggests that in ovo feeding of L-Leu may afford thermotolerance in broilers. CI - (c) 2018 Poultry Science Association Inc. FAU - Han, G AU - Han G AD - Laboratory of Stress Physiology and Metabolism, Faculty of Arts and Science, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka 819-0395, Japan. AD - Laboratory of Regulation in Metabolism and Behavior, Graduate School of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Kyushu University, Fukuoka 819-0395, Japan. FAU - Yang, H AU - Yang H AD - Laboratory of Regulation in Metabolism and Behavior, Graduate School of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Kyushu University, Fukuoka 819-0395, Japan. FAU - Wang, Y AU - Wang Y AD - Laboratory of Stress Physiology and Metabolism, Faculty of Arts and Science, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka 819-0395, Japan. AD - Laboratory of Regulation in Metabolism and Behavior, Graduate School of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Kyushu University, Fukuoka 819-0395, Japan. FAU - Zhang, R AU - Zhang R AD - Laboratory of Regulation in Metabolism and Behavior, Graduate School of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Kyushu University, Fukuoka 819-0395, Japan. AD - Division of Endocrinology, Department of Medicine, Boston Children's Hospital, Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA. FAU - Tashiro, K AU - Tashiro K AD - Laboratory of Molecular Gene Technique, Faculty of Agriculture, Kyushu University, Fukuoka 819-0395, Japan. FAU - Bungo, T AU - Bungo T AD - Laboratory of Animal Behavior and Physiology, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan. FAU - Furuse, M AU - Furuse M AD - Laboratory of Regulation in Metabolism and Behavior, Graduate School of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Kyushu University, Fukuoka 819-0395, Japan. FAU - Chowdhury, V S AU - Chowdhury VS AD - Laboratory of Stress Physiology and Metabolism, Faculty of Arts and Science, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka 819-0395, Japan. LA - eng PT - Journal Article PL - England TA - Poult Sci JT - Poultry science JID - 0401150 RN - 0 (Amino Acids) RN - 0 (HSP70 Heat-Shock Proteins) RN - 0 (HSP90 Heat-Shock Proteins) RN - 0 (RNA, Messenger) RN - EC 2.7.11.1 (TOR Serine-Threonine Kinases) RN - GMW67QNF9C (Leucine) SB - IM MH - Amino Acids/metabolism MH - Animals MH - Chick Embryo/drug effects MH - Chickens/growth & development/metabolism/*physiology MH - HSP70 Heat-Shock Proteins/genetics/*metabolism MH - HSP90 Heat-Shock Proteins/genetics/*metabolism MH - Leucine/administration & dosage/*pharmacology MH - Male MH - Ovum MH - RNA, Messenger/genetics/metabolism MH - TOR Serine-Threonine Kinases MH - Thermotolerance OTO - NOTNLM OT - in ovo feeding OT - L-leucine OT - chick OT - heat-shock protein OT - mammalian target of rapamycin EDAT- 2018/09/29 06:00 MHDA- 2019/03/12 06:00 CRDT- 2018/09/29 06:00 PHST- 2018/04/21 00:00 [received] PHST- 2018/08/31 00:00 [accepted] PHST- 2018/09/29 06:00 [pubmed] PHST- 2019/03/12 06:00 [medline] PHST- 2018/09/29 06:00 [entrez] AID - S0032-5791(19)30398-0 [pii] AID - 10.3382/ps/pey444 [doi] PST - ppublish SO - Poult Sci. 2019 Mar 1;98(3):1243-1253. doi: 10.3382/ps/pey444.