PMID- 30268830
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20181211
IS  - 1096-0295 (Electronic)
IS  - 0273-2300 (Linking)
VI  - 99
DP  - 2018 Nov
TI  - Are skin sensitisation test methods relevant for proteins?
PG  - 244-248
LID - S0273-2300(18)30250-2 [pii]
LID - 10.1016/j.yrtph.2018.09.028 [doi]
AB  - Tests for identification of chemical skin sensitisation hazard have been 
      available for decades, evolving from guinea pig assays, through the first 
      validated method, the local lymph node assay, to several validated in vitro 
      methods. These methods successfully identify the hazard for chemicals, with an 
      accuracy in the region of 85%. However, in some regulations, consideration may be 
      given to their application to proteins. Here, the scientific relevance of the use 
      of skin sensitisation tests for the assessment of the allergenic potential of 
      proteins is reviewed and considered in the context of both: (a) what is known of 
      the allergenic properties of proteins compared with chemicals, and (b) current 
      understanding of the extent to which proteins actually give rise to contact 
      allergy. There is no doubt that many foreign proteins can behave as respiratory 
      sensitisers and food allergens, and that certain proteins can also cause 
      cutaneous allergy via skin contact, typically mediated via immunoglobulin E (IgE) 
      antibody. However, the absence of any specificity in predictions from existing 
      skin sensitisation test methods, together with the lack of a suitable body of 
      either positive or negative controls, dictates that use of these tests with 
      proteins is without any scientific justification or predictive merit.
CI  - Copyright (c) 2018. Published by Elsevier Inc.
FAU - Basketter, David A
AU  - Basketter DA
AD  - DABMEB Consultancy Ltd, Sharnbrook, MK44 1PR, UK. Electronic address: 
      dabmebconsultancyltd@me.com.
FAU - Kimber, Ian
AU  - Kimber I
AD  - Faculty of Biology, Medicine and Health, University of Manchester, Manchester, 
      M13 9PT, UK.
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20180927
PL  - Netherlands
TA  - Regul Toxicol Pharmacol
JT  - Regulatory toxicology and pharmacology : RTP
JID - 8214983
RN  - 0 (Allergens)
RN  - 0 (Hazardous Substances)
RN  - 0 (Proteins)
SB  - IM
MH  - Allergens/immunology
MH  - Animals
MH  - Biological Assay/methods
MH  - Hazardous Substances/*immunology
MH  - Humans
MH  - Proteins/*immunology
MH  - Skin/*immunology
MH  - Skin Tests/*methods
OTO - NOTNLM
OT  - Enzymes
OT  - Hazard identification
OT  - Proteins
OT  - Skin sensitisation
EDAT- 2018/10/01 06:00
MHDA- 2018/12/12 06:00
CRDT- 2018/10/01 06:00
PHST- 2018/06/29 00:00 [received]
PHST- 2018/09/24 00:00 [revised]
PHST- 2018/09/26 00:00 [accepted]
PHST- 2018/10/01 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
PHST- 2018/10/01 06:00 [entrez]
AID - S0273-2300(18)30250-2 [pii]
AID - 10.1016/j.yrtph.2018.09.028 [doi]
PST - ppublish
SO  - Regul Toxicol Pharmacol. 2018 Nov;99:244-248. doi: 10.1016/j.yrtph.2018.09.028. 
      Epub 2018 Sep 27.