PMID- 30269789
OWN - NLM
STAT- MEDLINE
DCOM- 20190130
LR  - 20190130
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 224
DP  - 2018 Oct
TI  - Phages bearing specific peptides with affinity for porcine reproductive and 
      respiratory syndrome virus GP4 protein prevent cell penetration of the virus.
PG  - 43-49
LID - S0378-1135(18)30752-1 [pii]
LID - 10.1016/j.vetmic.2018.08.027 [doi]
AB  - Porcine reproductive and respiratory syndrome (PRRS) has caused significant 
      economic losses to the pig industry worldwide over the last 30 years. GP4 is a 
      minor highly glycosylated structural protein composed of 187 and 183 amino acids 
      in types I and II porcine reproductive and respiratory syndrome virus (PRRSV), 
      respectively. The GP4 protein co-localizes with cluster of differentiation 163 
      (CD163), the major receptor on the target cell membrane, to mediate PRRSV 
      internalization and disassembly. However, it remains to be established whether 
      blocking interactions between GP4 and host cells can inhibit viral proliferation. 
      In the present study, recombinant GP4 protein prepared and purified using the 
      Escherichia coli system effectively recognized PRRSV-positive serum. Phage 
      display biopanning on GP4 protein showed that the specific phages obtained could 
      distinguish PRRSV from the other viruses. The exogenous peptide WHEYPLVWLSGY 
      displayed on one of the candidate phages showed high affinity for GP4 protein and 
      exerted a significant inhibitory effect on PRRSV penetration in vitro. Moreover, 
      the N-terminus of GP4 was predicted as the critical receptor binding site and the 
      beginning of the fifth scavenger receptor cysteine-rich domain of CD163 as the 
      critical ligand recognition site based on sequence alignment and model prediction 
      analyses. The current study expands our understanding of PRRSV GP4 and its 
      receptor CD163 and provides a fresh perspective for the development of novel 
      peptide-based viral inhibition reagents.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Chen, Wanli
AU  - Chen W
AD  - College of Veterinary Medicine, South China Agricultural University, Guangzhou, 
      Guangdong Province, 510642, People's Republic of China; Guangdong Provincial Key 
      Laboratory of Prevention and Control for Severe Clinical Animal Diseases, 
      Guangzhou, Guangdong Province, 510642, People's Republic of China.
FAU - Cui, Jin
AU  - Cui J
AD  - College of Veterinary Medicine, South China Agricultural University, Guangzhou, 
      Guangdong Province, 510642, People's Republic of China; Key Laboratory of 
      Zoonosis Prevention and Control of Guangdong Province, Guangzhou, Guangdong 
      Province, 510642, People's Republic of China.
FAU - Wang, Jingyu
AU  - Wang J
AD  - College of Veterinary Medicine, South China Agricultural University, Guangzhou, 
      Guangdong Province, 510642, People's Republic of China; Guangdong Provincial Key 
      Laboratory of Prevention and Control for Severe Clinical Animal Diseases, 
      Guangzhou, Guangdong Province, 510642, People's Republic of China.
FAU - Sun, Yankou
AU  - Sun Y
AD  - College of Veterinary Medicine, South China Agricultural University, Guangzhou, 
      Guangdong Province, 510642, People's Republic of China; Key Laboratory of 
      Zoonosis Prevention and Control of Guangdong Province, Guangzhou, Guangdong 
      Province, 510642, People's Republic of China.
FAU - Ji, Chihai
AU  - Ji C
AD  - College of Veterinary Medicine, South China Agricultural University, Guangzhou, 
      Guangdong Province, 510642, People's Republic of China; Key Laboratory of 
      Zoonosis Prevention and Control of Guangdong Province, Guangzhou, Guangdong 
      Province, 510642, People's Republic of China.
FAU - Song, Ruyue
AU  - Song R
AD  - College of Veterinary Medicine, South China Agricultural University, Guangzhou, 
      Guangdong Province, 510642, People's Republic of China; Guangdong Provincial Key 
      Laboratory of Prevention and Control for Severe Clinical Animal Diseases, 
      Guangzhou, Guangdong Province, 510642, People's Republic of China.
FAU - Zeng, Yuchen
AU  - Zeng Y
AD  - College of Veterinary Medicine, South China Agricultural University, Guangzhou, 
      Guangdong Province, 510642, People's Republic of China; Guangdong Provincial Key 
      Laboratory of Prevention and Control for Severe Clinical Animal Diseases, 
      Guangzhou, Guangdong Province, 510642, People's Republic of China.
FAU - Pan, Haoming
AU  - Pan H
AD  - College of Veterinary Medicine, South China Agricultural University, Guangzhou, 
      Guangdong Province, 510642, People's Republic of China; Guangdong Provincial Key 
      Laboratory of Prevention and Control for Severe Clinical Animal Diseases, 
      Guangzhou, Guangdong Province, 510642, People's Republic of China.
FAU - Sheng, Jinliang
AU  - Sheng J
AD  - College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 
      Province, 832000, People's Republic of China.
FAU - Zhang, Guihong
AU  - Zhang G
AD  - College of Veterinary Medicine, South China Agricultural University, Guangzhou, 
      Guangdong Province, 510642, People's Republic of China; Key Laboratory of 
      Zoonosis Prevention and Control of Guangdong Province, Guangzhou, Guangdong 
      Province, 510642, People's Republic of China. Electronic address: 
      guihongzh@scau.edu.cn.
FAU - Wang, Heng
AU  - Wang H
AD  - College of Veterinary Medicine, South China Agricultural University, Guangzhou, 
      Guangdong Province, 510642, People's Republic of China; Guangdong Provincial Key 
      Laboratory of Prevention and Control for Severe Clinical Animal Diseases, 
      Guangzhou, Guangdong Province, 510642, People's Republic of China. Electronic 
      address: wangheng2009@scau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20180829
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD163 antigen)
RN  - 0 (Peptides)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Virus)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Viral Envelope Proteins)
SB  - IM
MH  - Animals
MH  - Antigens, CD/genetics/metabolism
MH  - Antigens, Differentiation, Myelomonocytic/genetics/metabolism
MH  - Bacteriophages/*chemistry/*physiology
MH  - Binding Sites
MH  - Cell Line
MH  - Peptides/*metabolism
MH  - Porcine Reproductive and Respiratory Syndrome/virology
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Protein Binding
MH  - Receptors, Cell Surface/genetics/metabolism
MH  - Receptors, Virus/metabolism
MH  - Recombinant Proteins/metabolism
MH  - Swine
MH  - *Viral Envelope Proteins/metabolism
MH  - Virus Internalization
OTO - NOTNLM
OT  - GP4 protein
OT  - Phage display
OT  - Porcine reproductive and respiratory syndrome
OT  - Viral inhibition
EDAT- 2018/10/03 06:00
MHDA- 2019/01/31 06:00
CRDT- 2018/10/02 06:00
PHST- 2018/06/27 00:00 [received]
PHST- 2018/08/20 00:00 [revised]
PHST- 2018/08/28 00:00 [accepted]
PHST- 2018/10/02 06:00 [entrez]
PHST- 2018/10/03 06:00 [pubmed]
PHST- 2019/01/31 06:00 [medline]
AID - S0378-1135(18)30752-1 [pii]
AID - 10.1016/j.vetmic.2018.08.027 [doi]
PST - ppublish
SO  - Vet Microbiol. 2018 Oct;224:43-49. doi: 10.1016/j.vetmic.2018.08.027. Epub 2018 
      Aug 29.