PMID- 30274007
OWN - NLM
STAT- MEDLINE
DCOM- 20190916
LR  - 20191210
IS  - 1433-6510 (Print)
IS  - 1433-6510 (Linking)
VI  - 64
IP  - 9
DP  - 2018 Sep 1
TI  - Effects of Freeze-Thaw Times on Screening Coagulation Tests and Factors VIII and 
      IX Activities in Citrate-Anticoagulated Plasma at -20 degrees C and -80 degrees C.
PG  - 1439-1444
LID - 10.7754/Clin.Lab.2018.180309 [doi]
AB  - BACKGROUND: Accurate determination of screening coagulation tests and factors 
      VIII and IX activities (FVIII:C and FIX:C) in fresh plasma is very important for 
      diagnosing abnormalities in the intrinsic or extrinsic coagulation pathways and 
      factor deficiencies. If thawed samples cannot be detected for all required items 
      at the same time, or need to be re-tested or re-stored, the thawed samples need 
      to be re-frozen. We planned to perform in-house validation studies on 
      freeze-thawed samples for screening coagulation tests, FVIII:C and FIX:C. 
      METHODS: Mean percent changes, numbers of samples with > 10% changes, and 
      difference plots were evaluated to determine clinically relevant differences 
      between results for fresh and freeze-thawed samples. The statistical significance 
      of differences between repeated-measure multiple groups and baseline values were 
      evaluated by repeated-measures analysis of variance. RESULTS: The acceptable 
      freeze-thaw cycles for activated partial thromboplastin time, fibrinogen, 
      prothrombin time/international normalized ratio, thrombin time, and FIX:C were 
      three times at -20 degrees C/-80 degrees C, while the acceptable freeze-thaw cycles for FVIII:C 
      were three times at -80 degrees C and once at -20 degrees C. CONCLUSIONS: The freeze-thaw results 
      on stabilities were affected by time and temperature, with lower temperature and 
      fewer times associated with more stable activity.
FAU - Feng, Guofang
AU  - Feng G
FAU - Zhao, Ying
AU  - Zhao Y
FAU - Zhang, Jie
AU  - Zhang J
FAU - Feng, Limin
AU  - Feng L
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Validation Study
PL  - Germany
TA  - Clin Lab
JT  - Clinical laboratory
JID - 9705611
RN  - 0 (Anticoagulants)
RN  - 0 (Citrates)
RN  - 839MOZ74GK (F8 protein, human)
RN  - 9001-27-8 (Factor VIII)
RN  - 9001-28-9 (Factor IX)
RN  - 9001-32-5 (Fibrinogen)
SB  - IM
MH  - Adult
MH  - Anticoagulants/*pharmacology
MH  - Blood Coagulation/*drug effects
MH  - Blood Coagulation Tests/*methods
MH  - Blood Specimen Collection/*methods
MH  - Citrates/*pharmacology
MH  - *Cold Temperature
MH  - Factor IX/*metabolism
MH  - Factor VIII/*metabolism
MH  - Female
MH  - Fibrinogen/metabolism
MH  - Freezing
MH  - Humans
MH  - International Normalized Ratio
MH  - Male
MH  - Middle Aged
MH  - Partial Thromboplastin Time
MH  - Predictive Value of Tests
MH  - Protein Stability
MH  - Proteolysis
MH  - Prothrombin Time
MH  - Reproducibility of Results
MH  - Thrombin Time
MH  - Time Factors
MH  - Young Adult
EDAT- 2018/10/03 06:00
MHDA- 2019/09/17 06:00
CRDT- 2018/10/02 06:00
PHST- 2018/10/02 06:00 [entrez]
PHST- 2018/10/03 06:00 [pubmed]
PHST- 2019/09/17 06:00 [medline]
AID - 10.7754/Clin.Lab.2018.180309 [doi]
PST - ppublish
SO  - Clin Lab. 2018 Sep 1;64(9):1439-1444. doi: 10.7754/Clin.Lab.2018.180309.