PMID- 30274142
OWN - NLM
STAT- MEDLINE
DCOM- 20181214
LR  - 20181214
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 10
IP  - 10
DP  - 2018 Sep 28
TI  - Ostreid Herpesvirus-1 Infects Specific Hemocytes in Ark Clam, Scapharca 
      broughtonii.
LID - 10.3390/v10100529 [doi]
LID - 529
AB  - High levels of ostreid herpesvirus 1 (OsHV-1) were detected in hemocytes of 
      OsHV-1 infected mollusks. Mollusk hemocytes are comprised of different cell types 
      with morphological and functional heterogeneity. Granular cells are considered 
      the main immunocompetent hemocytes. This study aimed to ascertain if OsHV-1 
      infects specific types of hemocytes in ark clams. Types of hemocytes were first 
      characterized through microexamination and flow cytometry. In addition to a large 
      group of red cells, there were three types of recognizable granular cells in ark 
      clams. Type II granular cells were mostly found with OsHV-1 infection by 
      transmission electron microscope (TEM) examination, and represented the hemocyte 
      type that was susceptible to OsHV-1 infection. The subcellular location of OsHV-1 
      particles in apoptotic type II granular cells was further analyzed. Some OsHV-1 
      particles were free inside the apoptotic cells, which may contribute to OsHV-1 
      transmission among cells in the host, some particles were also found enclosed 
      inside apoptotic bodies. Apoptosis is an important part of the host defense 
      system, but might also be hijacked by OsHV-1 as a strategy to escape host immune 
      attack. Following this investigation, a primary culture of type II granular cells 
      with OsHV-1 infection would facilitate the research on the interaction between 
      OsHV-1 and mollusk hosts.
FAU - Xin, Lusheng
AU  - Xin L
AUID- ORCID: 0000-0002-4685-1076
AD  - Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Key 
      Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture, 
      Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Qingdao 266071, China. xinls@ysfri.ac.cn.
AD  - Function Laboratory for Marine Fisheries Science and Food Production Processes, 
      Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, 
      China. xinls@ysfri.ac.cn.
FAU - Li, Chen
AU  - Li C
AD  - Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Key 
      Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture, 
      Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Qingdao 266071, China. lichen@ysfri.ac.cn.
AD  - Function Laboratory for Marine Fisheries Science and Food Production Processes, 
      Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, 
      China. lichen@ysfri.ac.cn.
FAU - Bai, Changming
AU  - Bai C
AD  - Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Key 
      Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture, 
      Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Qingdao 266071, China. baicm@ysfri.ac.cn.
AD  - Function Laboratory for Marine Fisheries Science and Food Production Processes, 
      Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, 
      China. baicm@ysfri.ac.cn.
FAU - Wang, Chongming
AU  - Wang C
AD  - Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Key 
      Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture, 
      Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Qingdao 266071, China. wangcm@ysfri.ac.cn.
AD  - Function Laboratory for Marine Fisheries Science and Food Production Processes, 
      Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, 
      China. wangcm@ysfri.ac.cn.
LA  - eng
GR  - U1706204/National Natural Science Foundation of China/International
GR  - CARS-49/Earmarked Fund for Modern Agro-industry Technology Research 
      System/International
GR  - 20603022017007/the Special Scientific Research Funds for Central Non-profit 
      Institutes, Yellow Sea Fisheries Research Institute/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180928
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
RN  - Ostreid herpesvirus 1
SB  - IM
MH  - Animals
MH  - Apoptosis/*immunology
MH  - DNA Viruses/genetics/*immunology
MH  - Hemocytes/*virology
MH  - *Host-Pathogen Interactions
MH  - Immunity, Innate/immunology
MH  - Scapharca/*immunology/*virology
MH  - Viral Load/genetics
MH  - Virion/immunology
PMC - PMC6213218
OTO - NOTNLM
OT  - OsHV-1 infection
OT  - Scapharca broughtonii
OT  - apoptosis
OT  - hemocytes
OT  - ostreid herpesvirus 1
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript, and in the decision to publish the results.
EDAT- 2018/10/03 06:00
MHDA- 2018/12/15 06:00
PMCR- 2018/10/01
CRDT- 2018/10/03 06:00
PHST- 2018/08/17 00:00 [received]
PHST- 2018/09/24 00:00 [revised]
PHST- 2018/09/24 00:00 [accepted]
PHST- 2018/10/03 06:00 [entrez]
PHST- 2018/10/03 06:00 [pubmed]
PHST- 2018/12/15 06:00 [medline]
PHST- 2018/10/01 00:00 [pmc-release]
AID - v10100529 [pii]
AID - viruses-10-00529 [pii]
AID - 10.3390/v10100529 [doi]
PST - epublish
SO  - Viruses. 2018 Sep 28;10(10):529. doi: 10.3390/v10100529.