PMID- 30275203
OWN - NLM
STAT- MEDLINE
DCOM- 20181011
LR  - 20221207
IS  - 1791-7530 (Electronic)
IS  - 0250-7005 (Linking)
VI  - 38
IP  - 10
DP  - 2018 Oct
TI  - Comparative p16(IKN4A) Expression in Laryngeal Carcinoma and Cervical Cancer 
      Precursors: A Real-time Grid-based Immunocytochemistry Analysis.
PG  - 5805-5810
LID - 10.21873/anticanres.12920 [doi]
AB  - BACKGROUND/AIM: p16 (gene locus: 9p21) tumor suppressor gene is considered an 
      important biomarker for the progression and prognosis in a variety of 
      malignancies and pre-cancerous lesions, including high-risk (HR-) human papilloma 
      virus (HPV)-mediated squamous intraepithelial lesions (SILs), based on 
      cytological and the corresponding cervical intraepithelial neoplasia (CIN) 
      histopathological categorization. p16 acts as a cyclin-dependent kinase-4 
      inhibitor negatively regulating the cell cycle. In persistent HPV infection, E7 
      oncogenic protein binds retinoblastoma protein leading to its proteolytic 
      transformation, also triggering E2F dissociation, which increases DNA 
      transcription and progression to S phase. This mechanism promotes aberrant p16 
      over-expression. Our aim was to comparatively analyze p16 protein expression 
      patterns in laryngeal squamous cell carcinomas (LSCC) and also in SILs. MATERIALS 
      AND METHODS: Fifty (n=50) primary LSCCs tissues all non-HPV-dependent, and a set 
      of 50 liquid-based SILs, were analyzed by immunohistochemistry and 
      immunocytochemistry, respectively. Concerning the screening process in 
      cytological slides, a novel real-time reference and calibration grid platform was 
      implemented and employed. RESULTS: Decreased protein expression was observed in 
      34/50 (68%) tissues regarding LSCCs. Overall p16 expression was associated to 
      smoking status of the patients (p=0.001), and also with the p-stage of the 
      examined malignancies (p=0.033). A strong statistical significance was assessed 
      correlating LSIL/HSIL cases with a progressive p16 over expression (p=0.001), 
      also reflecting a higher CIN diagnosis (p=0.001). CONCLUSION: p16 down-regulation 
      is a frequent genetic event in LSCCs, which is associated with advanced disease. 
      In contrast to this, p16 over-expression triggered by a specific molecular 
      mechanism shows a strong relationship with a progressively aggressive phenotype 
      due to upgraded SIL/CIN cervical categorization. The first described application 
      of the grid platform demonstrated a considerable improvement in the 
      immunocytochemistry slide screening process enhancing the diagnostic reliability.
CI  - Copyright(c) 2018, International Institute of Anticancer Research (Dr. George J. 
      Delinasios), All rights reserved.
FAU - Tsiambas, Evangelos
AU  - Tsiambas E
AD  - Department of Pathology-Cytology, 401 GA Hospital, Athens, Greece 
      tsiambasecyto@yahoo.gr.
AD  - Department of Pathology, Medical School, University of Ioannina, Ioannina, 
      Greece.
FAU - Riziotis, Christos
AU  - Riziotis C
AD  - Theoretical and Physical Chemistry Institute, National Hellenic Research 
      Foundation, Athens, Greece.
FAU - Mastronikolis, Nicholas S
AU  - Mastronikolis NS
AD  - ENT Department, Medical School, University of Patras, Patras, Greece.
FAU - Peschos, Dimitrios
AU  - Peschos D
AD  - Department of Physiology, Medical School, University of Ioannina, Ioannina, 
      Greece.
FAU - Mortakis, Alexandros
AU  - Mortakis A
AD  - LITO and IASO-REA, Private Gynaecological Centers, Athens, Greece.
FAU - Kyroysis, Grigorios
AU  - Kyroysis G
AD  - LITO and IASO-REA, Private Gynaecological Centers, Athens, Greece.
FAU - Mastronikolis, Stylianos N
AU  - Mastronikolis SN
AD  - Department of Medicine, School of Health Sciences, University of Crete, 
      Heraklion, Greece.
FAU - Batistatou, Anna
AU  - Batistatou A
AD  - Department of Pathology, Medical School, University of Ioannina, Ioannina, 
      Greece.
FAU - Lazaris, Andreas C
AU  - Lazaris AC
AD  - Department of Pathology, Medical School, University of Ioannina, Ioannina, 
      Greece.
FAU - Patsouris, Efstratios
AU  - Patsouris E
AD  - Department of Pathology, Medical School, University of Ioannina, Ioannina, 
      Greece.
FAU - Ragos, Vasileios
AU  - Ragos V
AD  - Department of Maxillofacial, Medical School, University of Ioannina, Ioannina, 
      Greece.
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (CDKN2A protein, human)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p16)
SB  - IM
MH  - Biomarkers, Tumor/*metabolism
MH  - Carcinoma, Squamous Cell/*metabolism/pathology
MH  - Cyclin-Dependent Kinase Inhibitor p16/*metabolism
MH  - Female
MH  - Follow-Up Studies
MH  - Humans
MH  - Immunohistochemistry
MH  - Laryngeal Neoplasms/*metabolism/pathology
MH  - Male
MH  - Prognosis
MH  - Squamous Intraepithelial Lesions of the Cervix/*metabolism/pathology
MH  - Uterine Cervical Neoplasms/*metabolism/pathology
MH  - Uterine Cervical Dysplasia/*metabolism/pathology
OTO - NOTNLM
OT  - cervical intraepithelial neoplasia
OT  - grid platform
OT  - histological categorization
OT  - laryngeal squamous cell carcinoma
OT  - p16
OT  - squamous intraepithelial lesion
EDAT- 2018/10/03 06:00
MHDA- 2018/10/12 06:00
CRDT- 2018/10/03 06:00
PHST- 2018/08/31 00:00 [received]
PHST- 2018/09/22 00:00 [revised]
PHST- 2018/09/24 00:00 [accepted]
PHST- 2018/10/03 06:00 [entrez]
PHST- 2018/10/03 06:00 [pubmed]
PHST- 2018/10/12 06:00 [medline]
AID - 38/10/5805 [pii]
AID - 10.21873/anticanres.12920 [doi]
PST - ppublish
SO  - Anticancer Res. 2018 Oct;38(10):5805-5810. doi: 10.21873/anticanres.12920.