PMID- 30278995
OWN - NLM
STAT- MEDLINE
DCOM- 20190208
LR  - 20211204
IS  - 1618-0372 (Electronic)
IS  - 0065-1281 (Linking)
VI  - 120
IP  - 8
DP  - 2018 Nov
TI  - Differential expression of mTOR signaling pathway proteins in lichen planopilaris 
      and frontal fibrosing alopecia.
PG  - 837-845
LID - S0065-1281(18)30175-2 [pii]
LID - 10.1016/j.acthis.2018.09.007 [doi]
AB  - Dysregulation of the mammalian target of rapamycin (mTOR) signaling pathway has a 
      variety of effects on the immune system and stem cell proliferation. Lichen 
      planopilaris (LPP) and frontal fibrosing alopecia (FFA) are inflammatory scalp 
      conditions resulting in permanent alopecia, which are thought to be related to 
      stem cell damage. Here we investigate the expression of mTOR signaling pathway 
      proteins in human hair follicles of LPP and FFA patients. The expression of mTOR 
      pathway proteins in biopsy specimens from lesional and non-lesional scalp areas 
      of eight LPP and five FFA patients were compared to control scalp biopsies from 
      patients undergoing surgical excisions of sebaceous cysts. We performed 
      immunohistochemical evaluation using a panel of antibodies including mTOR, 
      phospho-mTOR (Ser2448), phospho-p70S6K (Thr389), phospho-4EBP1 (Thr37146), and 
      phospho-tuberin (T1462), as well as Western blot analysis for phospho-p70S6K 
      (Thr389) expression. All evaluated mTOR pathway proteins were similarly expressed 
      in the control and patient non-lesional scalps. While mTOR expression did not 
      show significant alterations between the groups, p-mTOR, p-p70S6K, p-4EBP1, and 
      p-tuberin expressions decreased in the interfollicular epidermis in the lesional 
      scalps of patients. p-p70S6K and p-4EBP1 expression decreased in the outer root 
      sheath (ORS) and inner root sheath (IRS) of the bulge of hair follicles in the 
      lesional scalps of patients. p-mTOR and p-p70S6K expression increased in the 
      lower follicle ORS and bulb of the hair follicles, and p-4EBP1 expression 
      decreased in the bulb of the hair follicles in the lesional scalps of patients. 
      Phospho-tuberin expression increased in the IRS of the bulge and lower follicle 
      ORS of the hair follicles in the lesional scalps of patients, whereas its 
      expression decreased in the bulb. Our results indicate that the mTOR signaling 
      pathway proteins are localized throughout normal hair follicles and that 
      expression of mTOR signaling pathway proteins is altered in the hair follicles of 
      LPP and FFA patients. Further research is required to understand the mechanism by 
      which mTOR operates in the pathogenesis of these diseases.
CI  - Copyright (c) 2018 Elsevier GmbH. All rights reserved.
FAU - Dicle, Ozlem
AU  - Dicle O
AD  - Akdeniz University School of Medicine, Department of Dermatology and Venereology, 
      Antalya, Turkey. Electronic address: odicle@akdeniz.edu.tr.
FAU - Celik-Ozenci, Ciler
AU  - Celik-Ozenci C
AD  - Akdeniz University School of Medicine, Department of Histology and Embryology, 
      Antalya, Turkey.
FAU - Sahin, Pinar
AU  - Sahin P
AD  - Akdeniz University School of Medicine, Department of Histology and Embryology, 
      Antalya, Turkey.
FAU - Pfannes, Eva Katharina Barbosa
AU  - Pfannes EKB
AD  - Charite-Universitatsmedizin, Department of Dermatology and Allergy, Clinical 
      Research Center for Hair and Skin Science, Berlin, Germany.
FAU - Vogt, Annika
AU  - Vogt A
AD  - Charite-Universitatsmedizin, Department of Dermatology and Allergy, Clinical 
      Research Center for Hair and Skin Science, Berlin, Germany.
FAU - Altinok, Berna Nazlim
AU  - Altinok BN
AD  - Akdeniz University School of Medicine, Department of Dermatology and Venereology, 
      Antalya, Turkey.
FAU - Blume-Peytavi, Ulrike
AU  - Blume-Peytavi U
AD  - Charite-Universitatsmedizin, Department of Dermatology and Allergy, Clinical 
      Research Center for Hair and Skin Science, Berlin, Germany.
LA  - eng
PT  - Journal Article
DEP - 20180929
PL  - Germany
TA  - Acta Histochem
JT  - Acta histochemica
JID - 0370320
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - Lichen planus follicularis
SB  - IM
MH  - *Alopecia/metabolism/pathology
MH  - Blotting, Western
MH  - Female
MH  - Hair Follicle/pathology
MH  - Humans
MH  - Immunohistochemistry
MH  - *Lichen Planus/metabolism
MH  - Reference Standards
MH  - Signal Transduction
MH  - TOR Serine-Threonine Kinases/*metabolism
OTO - NOTNLM
OT  - Frontal fibrosing alopecia
OT  - Hair follicle
OT  - Lichen planopilaris
OT  - Primary cicatricial alopecia
OT  - mTOR signaling pathway
EDAT- 2018/10/04 06:00
MHDA- 2019/02/09 06:00
CRDT- 2018/10/04 06:00
PHST- 2018/05/22 00:00 [received]
PHST- 2018/09/20 00:00 [revised]
PHST- 2018/09/20 00:00 [accepted]
PHST- 2018/10/04 06:00 [pubmed]
PHST- 2019/02/09 06:00 [medline]
PHST- 2018/10/04 06:00 [entrez]
AID - S0065-1281(18)30175-2 [pii]
AID - 10.1016/j.acthis.2018.09.007 [doi]
PST - ppublish
SO  - Acta Histochem. 2018 Nov;120(8):837-845. doi: 10.1016/j.acthis.2018.09.007. Epub 
      2018 Sep 29.