PMID- 30280947
OWN - NLM
STAT- MEDLINE
DCOM- 20190626
LR  - 20190626
IS  - 1563-5279 (Electronic)
IS  - 0020-7454 (Linking)
VI  - 129
IP  - 3
DP  - 2019 Mar
TI  - Site specific hypermethylation of CpGs in Connexin genes 30, 26 and 43 in 
      different grades of glioma and attenuated levels of their mRNAs.
PG  - 273-282
LID - 10.1080/00207454.2018.1526802 [doi]
AB  - AIM: Gliomas, the intracranial tumours are considered the deadliest malignancies. 
      The gap junctional Connexins (Cxs) that maintain cellular homeostasis perform a 
      unique function in glial tumour suppression. However, the differential 
      methylation patterns of Cxs were not revealed in glioma so far. The current study 
      attempts to categorise promoter methylation of Cx30 and Cx26 and intron 
      methylation of Cx43 in different grades of human glioma. MATERIALS AND METHODS: 
      About 85 glioma patients with pathologically confirmed grades and 15 control 
      brain tissues were recruited in the study. Bisulphite-PCR-Single Stranded 
      Conformation analysis(SSCA), Bisulphite sequencing and MeDIP-qPCR were carried 
      out to assess methylation status and Cx mRNA levels were also analysed to 
      evaluate the effect of methylation. RESULTS: We found that promoter CpG 
      islands(CpGs) reside in Sp1 and Ap2 sites of Cx30 and 26 were hypermethylated in 
      high grades (HG) of glioma rather than low grades. The input % of both was 
      significantly increased (p < 0.03) in progressive grades. Interestingly, Cx43 
      could exhibit a significant increase (p < 0.05) in input % only in grade IV. 
      While, Cx30 and 26 mRNAs were downregulated according to their methylation status 
      in progressive fashion with grades, Cx43 was downregulated irrespective of intron 
      methylation. CONCLUSION: Thus, we suggest that the sites and extent of 
      methylation of Cxs (30 and 26 but not in 43) are found to be altered. In 
      different grades of glioma can provide better appreciation of the grade of the 
      patient and might help in strategies based on epigenetic approaches.
FAU - J, Jayalakshmi
AU  - J J
AD  - a Department of Biochemistry , University of Madras , Chennai , Tamilnadu , 
      India.
FAU - Vanisree, Arambakkam Janardhanam
AU  - Vanisree AJ
AD  - a Department of Biochemistry , University of Madras , Chennai , Tamilnadu , 
      India.
FAU - Ravisankar, Shantha
AU  - Ravisankar S
AD  - b Department of Neuropathology , Tamilnadu Multispeciality Hospital , Chennai , 
      Tamilnadu , India.
FAU - K, Rama
AU  - K R
AD  - c Department of Neuropathology , Madras Medical College and Government General 
      hospital , Chennai , Tamilnadu , India.
LA  - eng
PT  - Journal Article
DEP - 20181023
PL  - England
TA  - Int J Neurosci
JT  - The International journal of neuroscience
JID - 0270707
RN  - 0 (Connexin 30)
RN  - 0 (Connexin 43)
RN  - 0 (GJA1 protein, human)
RN  - 0 (RNA, Messenger)
RN  - 127120-53-0 (Connexin 26)
SB  - IM
MH  - Brain Neoplasms/genetics/*metabolism
MH  - Connexin 26/genetics/*metabolism
MH  - Connexin 30/genetics/*metabolism
MH  - Connexin 43/genetics/*metabolism
MH  - *CpG Islands/genetics
MH  - *DNA Methylation/genetics
MH  - Down-Regulation
MH  - Glioma/genetics/*metabolism
MH  - Humans
MH  - Introns
MH  - Neoplasm Grading
MH  - Promoter Regions, Genetic
MH  - RNA, Messenger/genetics/*metabolism
OTO - NOTNLM
OT  - Bisulphite sequencing
OT  - Connexin
OT  - CpG Island
OT  - MeDIP
OT  - methylation
EDAT- 2018/10/04 06:00
MHDA- 2019/06/27 06:00
CRDT- 2018/10/04 06:00
PHST- 2018/10/04 06:00 [pubmed]
PHST- 2019/06/27 06:00 [medline]
PHST- 2018/10/04 06:00 [entrez]
AID - 10.1080/00207454.2018.1526802 [doi]
PST - ppublish
SO  - Int J Neurosci. 2019 Mar;129(3):273-282. doi: 10.1080/00207454.2018.1526802. Epub 
      2018 Oct 23.