PMID- 30281676
OWN - NLM
STAT- MEDLINE
DCOM- 20190405
LR  - 20191210
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 13
IP  - 10
DP  - 2018
TI  - Rapid capture and detection of ostreid herpesvirus-1 from Pacific oyster 
      Crassostrea gigas and seawater using magnetic beads.
PG  - e0205207
LID - 10.1371/journal.pone.0205207 [doi]
LID - e0205207
AB  - Ostreid herpesvirus-1 (OsHV-1) has been involved in mass mortality episodes of 
      Pacific oysters Crassostrea gigas throughout the world, causing important 
      economic losses to the aquaculture industry. In the present study, magnetic beads 
      (MBs) coated with an anionic polymer were used to capture viable OsHV-1 from two 
      types of naturally infected matrix: oyster homogenate and seawater. Adsorption of 
      the virus on the MBs and characterisation of the MB-virus conjugates was 
      demonstrated by real-time quantitative PCR (qPCR). To study the infective 
      capacity of the captured virus, MB-virus conjugates were injected in the adductor 
      muscle of naive spat oysters, using oyster homogenate and seawater without MBs as 
      positive controls, and bare MBs and sterile water as negative controls. 
      Mortalities were induced after injection with MB-virus conjugates and in positive 
      controls, whereas no mortalities were recorded in negative controls. Subsequent 
      OsHV-1 DNA and RNA analysis of the oysters by qPCR and reverse transcription qPCR 
      (RT-qPCR), respectively, confirmed that the virus was the responsible for the 
      mortality event and the ability of the MBs to capture viable viral particles. The 
      capture of viable OsHV-1 using MBs is a rapid and easy isolation method and a 
      promising tool, combined with qPCR, to be applied to OsHV-1 detection in 
      aquaculture facilities.
FAU - Toldra, Anna
AU  - Toldra A
AD  - IRTA, Ctra., Sant Carles de la Rapita, Tarragona, Spain.
FAU - Andree, Karl B
AU  - Andree KB
AD  - IRTA, Ctra., Sant Carles de la Rapita, Tarragona, Spain.
FAU - Bertomeu, Edgar
AU  - Bertomeu E
AD  - IRTA, Ctra., Sant Carles de la Rapita, Tarragona, Spain.
FAU - Roque, Ana
AU  - Roque A
AD  - IRTA, Ctra., Sant Carles de la Rapita, Tarragona, Spain.
FAU - Carrasco, Noelia
AU  - Carrasco N
AD  - IRTA, Ctra., Sant Carles de la Rapita, Tarragona, Spain.
FAU - Gairin, Ignasi
AU  - Gairin I
AD  - IRTA, Ctra., Sant Carles de la Rapita, Tarragona, Spain.
FAU - Furones, M Dolores
AU  - Furones MD
AD  - IRTA, Ctra., Sant Carles de la Rapita, Tarragona, Spain.
FAU - Campas, Monica
AU  - Campas M
AUID- ORCID: 0000-0002-1220-7100
AD  - IRTA, Ctra., Sant Carles de la Rapita, Tarragona, Spain.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181003
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (DNA, Viral)
RN  - Ostreid herpesvirus 1
SB  - IM
MH  - Animals
MH  - Aquaculture/*methods
MH  - Crassostrea/*virology
MH  - DNA Viruses/genetics/*isolation & purification/pathogenicity
MH  - DNA, Viral/isolation & purification
MH  - Immunomagnetic Separation/methods
MH  - Pacific Ocean
MH  - Real-Time Polymerase Chain Reaction
MH  - Seafood/*virology
MH  - Seawater/*virology
MH  - Time Factors
PMC - PMC6169968
COIS- The authors have declared that no competing interests exist.
EDAT- 2018/10/04 06:00
MHDA- 2019/04/06 06:00
PMCR- 2018/10/03
CRDT- 2018/10/04 06:00
PHST- 2018/07/02 00:00 [received]
PHST- 2018/09/20 00:00 [accepted]
PHST- 2018/10/04 06:00 [entrez]
PHST- 2018/10/04 06:00 [pubmed]
PHST- 2019/04/06 06:00 [medline]
PHST- 2018/10/03 00:00 [pmc-release]
AID - PONE-D-18-19617 [pii]
AID - 10.1371/journal.pone.0205207 [doi]
PST - epublish
SO  - PLoS One. 2018 Oct 3;13(10):e0205207. doi: 10.1371/journal.pone.0205207. 
      eCollection 2018.