PMID- 30282067
OWN - NLM
STAT- MEDLINE
DCOM- 20181102
LR  - 20191210
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 50
IP  - 1
DP  - 2018
TI  - Circular RNA Expression Profile in Laryngeal Squamous Cell Carcinoma Revealed by 
      Microarray.
PG  - 342-352
LID - 10.1159/000494010 [doi]
AB  - BACKGROUND/AIMS: A growing body of evidence has suggested that circular RNAs 
      (circRNAs) have crucial functions in the regulation of gene expression, and their 
      dysregulation has been implicated in various types of cancers. However, the roles 
      of circRNAs in laryngeal cancer remain largely unknown. This study investigated 
      the global changes in the expression pattern of circRNAs in laryngeal squamous 
      cell carcinoma (LSCC) to identify potential differentially expressed circRNAs. 
      METHODS: Microarray-based circRNA expression was determined in LSCC and paired 
      normal laryngeal tissues. Pathway analyses of the genes producing differentially 
      expressed circRNAs were performed to predict the function of circRNAs using 
      standard enrichment computational methods. Expression levels of candidate 
      circRNAs and microRNAs (miRNAs) were detected by quantitative real-time PCR. The 
      circRNA/ miRNA interactions were constructed using bioinformatics methods to 
      predict the binding of miRNA with circRNA. RESULTS: We identified 506 
      differentially expressed circRNAs from human LSCC and normal laryngeal mucosa 
      tissues. We confirmed that hsa_circ_0044520 and hsa_circ_0044529 were 
      significantly upregulated in LSCC tissues. The most likely potential target 
      miRNAs for hsa_ circ_0044520 and hsa_circ_0044529 were hsa-miR-4726-5p and 
      hsa-miR-4640-5p, respectively. Functional analysis showed that hsa_circ_0044520 
      and hsa_circ_0044529 were involved in the process of collagen synthesis. 
      CONCLUSION: Competitive endogenous RNA network prediction and bioinformatics 
      functional analysis revealed that hsa_circ_0044520 and hsa_circ_0044529 play 
      important regulatory roles by sponging hsa-miR-4726-5p and hsa-miR-4640-5p, 
      thereby providing novel insights into the tumorigenesis of LSCC.
CI  - (c) 2018 The Author(s). Published by S. Karger AG, Basel.
FAU - Fan, Yue
AU  - Fan Y
FAU - Xia, Xin
AU  - Xia X
FAU - Zhu, Yingying
AU  - Zhu Y
FAU - Diao, Wenwen
AU  - Diao W
FAU - Zhu, Xiaoli
AU  - Zhu X
FAU - Gao, Zhiqiang
AU  - Gao Z
FAU - Chen, Xingming
AU  - Chen X
LA  - eng
PT  - Journal Article
DEP - 20181003
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Carcinoma, Squamous Cell/genetics/*pathology
MH  - Computational Biology
MH  - Gene Regulatory Networks/genetics
MH  - Humans
MH  - Laryngeal Neoplasms/genetics/*pathology
MH  - Male
MH  - MicroRNAs/metabolism
MH  - Middle Aged
MH  - Oligonucleotide Array Sequence Analysis
MH  - RNA/*metabolism
MH  - RNA, Circular
MH  - Transcriptome
OTO - NOTNLM
OT  - Bioinformatics analysis
OT  - CircRNAs
OT  - Laryngeal squamous cell carcinoma
OT  - Microarray
OT  - miRNA sponges
EDAT- 2018/10/04 06:00
MHDA- 2018/11/06 06:00
CRDT- 2018/10/04 06:00
PHST- 2018/01/01 00:00 [received]
PHST- 2018/09/25 00:00 [accepted]
PHST- 2018/10/04 06:00 [pubmed]
PHST- 2018/11/06 06:00 [medline]
PHST- 2018/10/04 06:00 [entrez]
AID - 000494010 [pii]
AID - 10.1159/000494010 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2018;50(1):342-352. doi: 10.1159/000494010. Epub 2018 Oct 
      3.