PMID- 30282427
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2287-1152 (Print)
IS  - 2287-5603 (Electronic)
IS  - 2287-1152 (Linking)
VI  - 19
IP  - 3
DP  - 2018 Sep
TI  - Effects of three-dimensionally printed polycaprolactone/beta-tricalcium phosphate 
      scaffold on osteogenic differentiation of adipose tissue- and bone marrow-derived 
      stem cells.
PG  - 181-189
LID - 10.7181/acfs.2018.01879 [doi]
AB  - BACKGROUND: Autogenous bone grafts have several limitations including donor-site 
      problems and insufficient bone volume. To address these limitations, research on 
      bone regeneration is being conducted actively. In this study, we investigate the 
      effects of a three-dimensionally (3D) printed polycaprolactone (PCL)/tricalcium 
      phosphate (TCP) scaffold on the osteogenic differentiation potential of adipose 
      tissue-derived stem cells (ADSCs) and bone marrow-derived stem cells (BMSCs). 
      METHODS: We investigated the extent of osteogenic differentiation on the first 
      and tenth day and fourth week after cell culture. Cytotoxicity of the 3D printed 
      PCL/beta-TCP scaffold was evaluated by 
      3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium 
      assay, prior to osteogenic differentiation analysis. ADSCs and BMSCs were divided 
      into three groups: C, only cultured cells; M, cells cultured in the 3D printed 
      PCL/beta-TCP scaffold; D, cells cultured in the 3D printed PCL/beta-TCP scaffold with a 
      bone differentiation medium. Alkaline phosphatase (ALP) activity assay, von Kossa 
      staining, reverse transcription-polymerase chain reaction (RT-PCR), and Western 
      blotting were performed for comparative analysis. RESULTS: ALP assay and von 
      Kossa staining revealed that group M had higher levels of osteogenic 
      differentiation compared to group C. RT-PCR showed that gene expression was 
      higher in group M than in group C, indicating that, compared to group C, 
      osteogenic differentiation was more extensive in group M. Expression levels of 
      proteins involved in ossification were higher in group M, as per the Western 
      blotting results. CONCLUSION: Osteogenic differentiation was increased in 
      mesenchymal stromal cells (MSCs) cultured in the 3D printed PCL/TCP scaffold 
      compared to the control group. Osteogenic differentiation activity of MSCs 
      cultured in the 3D printed PCL/TCP scaffold was lower than that of cells cultured 
      on the scaffold in bone differentiation medium. Collectively, these results 
      indicate that the 3D printed PCL/TCP scaffold promoted osteogenic differentiation 
      of MSCs and may be widely used for bone tissue engineering.
FAU - Park, Hannara
AU  - Park H
AD  - Department of Plastic and Reconstructive Surgery, Daegu Fatima Hospital, Daegu, 
      Korea.
FAU - Kim, Jin Soo
AU  - Kim JS
AD  - Department of Plastic and Reconstructive Surgery, Daegu Fatima Hospital, Daegu, 
      Korea.
FAU - Oh, Eun Jung
AU  - Oh EJ
AD  - Department of Plastic and Reconstructive Surgery, School of Medicine, Kyungpook 
      National University, Daegu, Korea.
FAU - Kim, Tae Jung
AU  - Kim TJ
AD  - Department of Plastic and Reconstructive Surgery, School of Medicine, Kyungpook 
      National University, Daegu, Korea.
FAU - Kim, Hyun Mi
AU  - Kim HM
AD  - Department of Plastic and Reconstructive Surgery, School of Medicine, Kyungpook 
      National University, Daegu, Korea.
FAU - Shim, Jin Hyung
AU  - Shim JH
AD  - Department of Mechanical Engineering, Korea Polytechnic University, Siheung, 
      Korea.
FAU - Yoon, Won Soo
AU  - Yoon WS
AD  - Department of Mechanical Engineering, Korea Polytechnic University, Siheung, 
      Korea.
FAU - Huh, Jung Bo
AU  - Huh JB
AD  - Department of Prosthodontics, Dental Research Institute, Institute of 
      Translational Dental Science, School of Dentistry, Pusan National University, 
      Yangsan, Korea.
FAU - Moon, Sung Hwan
AU  - Moon SH
AD  - Department of Medicine, Konkuk University School of Medicine, Seoul, Korea.
FAU - Kang, Seong Soo
AU  - Kang SS
AD  - College of Veterinary Medicine, Chonnam National University, Gwangju, Korea.
FAU - Chung, Ho Yun
AU  - Chung HY
AD  - Department of Plastic and Reconstructive Surgery, School of Medicine, Kyungpook 
      National University, Daegu, Korea.
LA  - eng
PT  - Journal Article
DEP - 20180920
PL  - Korea (South)
TA  - Arch Craniofac Surg
JT  - Archives of craniofacial surgery
JID - 101588280
PMC - PMC6177683
OTO - NOTNLM
OT  - Adipose tissue
OT  - Bone marrow
OT  - Cell differentiation
OT  - Mesenchymal stromal cells
OT  - Polycaprolactone
OT  - Stem cells
OT  - Tricalcium phosphate
COIS- No potential conflict of interest relevant to this article was reported.
EDAT- 2018/10/05 06:00
MHDA- 2018/10/05 06:01
PMCR- 2018/09/01
CRDT- 2018/10/05 06:00
PHST- 2018/04/16 00:00 [received]
PHST- 2018/09/10 00:00 [accepted]
PHST- 2018/10/05 06:00 [entrez]
PHST- 2018/10/05 06:00 [pubmed]
PHST- 2018/10/05 06:01 [medline]
PHST- 2018/09/01 00:00 [pmc-release]
AID - acfs.2018.01879 [pii]
AID - acfs-2018-01879 [pii]
AID - 10.7181/acfs.2018.01879 [doi]
PST - ppublish
SO  - Arch Craniofac Surg. 2018 Sep;19(3):181-189. doi: 10.7181/acfs.2018.01879. Epub 
      2018 Sep 20.