PMID- 30284821
OWN - NLM
STAT- MEDLINE
DCOM- 20190729
LR  - 20190729
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 66
IP  - 41
DP  - 2018 Oct 17
TI  - Purification and Characterization of Naturally Occurring Post-Translationally 
      Cleaved Ara h 6, an Allergen That Contributes Substantially to the Allergenic 
      Potency of Peanut.
PG  - 10855-10863
LID - 10.1021/acs.jafc.8b03140 [doi]
AB  - The 2S albumin Ara h 6 is one of the most important peanut allergens. A 
      post-translationally cleaved Ara h 6 (pAra h 6) was purified from Virginia type 
      peanuts, and the cleavage site was mapped using high-resolution mass 
      spectrometry. Compared to intact Ara h 6, pAra h 6 lacks a 5-amino acid stretch, 
      resembling amino acids 43-47 (UniProt accession number Q647G9) in the 
      nonstructured loop. Consequently, pAra h 6 consists of two chains: an N-terminal 
      chain of approximately 5 kDa and a C-terminal chain of approximately 9 kDa, held 
      together by disulfide bonds. Intermediate post-translationally cleaved products, 
      in which this stretch is cleaved yet still attached to one of the subunits, are 
      also present. The secondary structure and immunoglobulin E (IgE) binding of pAra 
      h 6 resembles that of intact Ara h 6, indicating that the loss of the 
      nonstructured loop is not critical for maintaining the protein structure. 
      Commercially available monoclonal and polyclonal immunoglobulin G (IgG) 
      antibodies directed to Ara h 6 react with both intact Ara h 6 and pAra h 6, 
      suggesting that the involved epitopes are not located in the area that is 
      post-translationally cleaved. No differences between intact Ara h 6 and pAra h 6 
      in terms of IgE binding were found, suggesting that the area that is 
      post-translationally cleaved is not involved in IgE epitopes either. For all main 
      cultivars Runner, Virginia, Valencia, and Spanish, intact Ara h 6 and pAra h 6 
      occur in peanut at similar levels, indicating that pAra h 6 is a consistent and 
      important contributor to the allergenic potency of peanut.
FAU - de Jong, Govardus A H
AU  - de Jong GAH
AD  - TNO , Utrechtseweg 48 , 3704 HE , Zeist , The Netherlands.
FAU - Jayasena, Shyamali
AU  - Jayasena S
AD  - Food Allergy Research and Resource Program, Department of Food Science & 
      Technology , University of Nebraska , 279 Food Innovation Center , Lincoln , 
      Nebraska 68588-6207 , United States.
FAU - Johnson, Phil
AU  - Johnson P
AUID- ORCID: 0000-0002-0462-8933
AD  - Food Allergy Research and Resource Program, Department of Food Science & 
      Technology , University of Nebraska , 279 Food Innovation Center , Lincoln , 
      Nebraska 68588-6207 , United States.
FAU - Marsh, Justin
AU  - Marsh J
AD  - Food Allergy Research and Resource Program, Department of Food Science & 
      Technology , University of Nebraska , 279 Food Innovation Center , Lincoln , 
      Nebraska 68588-6207 , United States.
FAU - Apostolovic, Danijela
AU  - Apostolovic D
AD  - Immunology and Allergy Unit, Department of Medicine Solna , Karolinska Institutet 
      , 171 77 Stockholm , Sweden.
FAU - van Hage, Marianne
AU  - van Hage M
AD  - Immunology and Allergy Unit, Department of Medicine Solna , Karolinska Institutet 
      , 171 77 Stockholm , Sweden.
FAU - Nordlee, Julie
AU  - Nordlee J
AD  - TNO , Utrechtseweg 48 , 3704 HE , Zeist , The Netherlands.
FAU - Baumert, Joe
AU  - Baumert J
AD  - TNO , Utrechtseweg 48 , 3704 HE , Zeist , The Netherlands.
FAU - Taylor, Steve L
AU  - Taylor SL
AUID- ORCID: 0000-0002-7178-7520
AD  - TNO , Utrechtseweg 48 , 3704 HE , Zeist , The Netherlands.
FAU - Roucairol, Camille
AU  - Roucairol C
AD  - DBV Technologies , Development Department , 92120 Montrouge , France.
FAU - de Jongh, Harmen
AU  - de Jongh H
AD  - Protin consult, 3702 VL , Zeist , The Netherlands.
FAU - Koppelman, Stef J
AU  - Koppelman SJ
AUID- ORCID: 0000-0001-7995-1754
AD  - Food Allergy Research and Resource Program, Department of Food Science & 
      Technology , University of Nebraska , 279 Food Innovation Center , Lincoln , 
      Nebraska 68588-6207 , United States.
LA  - eng
PT  - Journal Article
DEP - 20181004
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (2S Albumins, Plant)
RN  - 0 (Amino Acids)
RN  - 0 (Antigens, Plant)
RN  - 0 (Ara h 6 allergen, Arachis hypogaea)
RN  - 0 (Epitopes)
RN  - 0 (Glycoproteins)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Plant Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
MH  - 2S Albumins, Plant/*chemistry/immunology/*isolation & purification
MH  - Amino Acid Sequence
MH  - Amino Acids/chemistry
MH  - Antigens, Plant/*chemistry/immunology/*isolation & purification
MH  - Arachis/*chemistry
MH  - Epitopes/immunology
MH  - Glycoproteins/chemistry/immunology/isolation & purification
MH  - Humans
MH  - Immunoglobulin E/immunology
MH  - Immunoglobulin G/immunology
MH  - Peanut Hypersensitivity/metabolism/prevention & control
MH  - Plant Proteins/chemistry/immunology/isolation & purification
MH  - Protein Structure, Secondary
OTO - NOTNLM
OT  - Arachis hypogaea
OT  - IgE
OT  - allergen
OT  - mass spectrometry
OT  - peanut
EDAT- 2018/10/05 06:00
MHDA- 2019/07/30 06:00
CRDT- 2018/10/05 06:00
PHST- 2018/10/05 06:00 [pubmed]
PHST- 2019/07/30 06:00 [medline]
PHST- 2018/10/05 06:00 [entrez]
AID - 10.1021/acs.jafc.8b03140 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2018 Oct 17;66(41):10855-10863. doi: 10.1021/acs.jafc.8b03140. 
      Epub 2018 Oct 4.