PMID- 30288860
OWN - NLM
STAT- MEDLINE
DCOM- 20190118
LR  - 20190118
IS  - 1095-8355 (Electronic)
IS  - 1065-6995 (Linking)
VI  - 42
IP  - 12
DP  - 2018 Dec
TI  - Loganin prevents BV-2 microglia cells from Abeta(1-42) -induced inflammation via 
      regulating TLR4/TRAF6/NF-kappaB axis.
PG  - 1632-1642
LID - 10.1002/cbin.11060 [doi]
AB  - Neuroinflammation is closely related with the pathogenesis and progress of 
      neurodegenerative diseases including Alzheimer's disease (AD). Loganin, an 
      iridoid glycoside obtained from traditional Chinese medicine Cornus officinalis, 
      has properties of inhibiting inflammation and improving memory. The present study 
      was aimed to investigate effects of loganin on Abeta-induced inflammation and to 
      explore the underlying mechanisms. BV-2 microglia cells were stimulated with 
      10 microM Abeta(1-42) for 24 h to induce inflammatory damage. According to results of 
      CCK-8 assay, the doses of loganin in present work were 10 and 30 microM. We found 
      that treatment with loganin could inhibit Abeta(1-42) -induced microglia activation. 
      Furthermore, loganin treatment prevented the over-production of Tumor necrosis 
      factor-alpha (TNF-alpha), Interleukin-6 (IL-6), Macrophage Chemotactic Protein 1(MCP-1), 
      Nitric oxide (NO), Prostaglandin E2 (PGE2) and the up-regulation of inducible 
      nitric oxide synthase (iNOS) and Cyclooxygenase 2 (COX-2) in Abeta(1-42) -stimulated 
      BV-2 cells. Results from Western blots demonstrated that loganin inhibited 
      Abeta(1-42) -induced elevation in Toll-like receptor 4 (TLR4), Myeloid 
      Differentiation Factor 88 (MyD88) and TNF receptor-associated factor 6 (TRAF6). 
      Loganin treatment also attenuated the increased phosphorylation level of IRAK4 
      caused by Abeta(1-42) . Additionally, loganin alleviated nuclear translocation of 
      NF-kappaB p65 subunit in Abeta(1-42) -stimulated BV-2 cells, and this phenomenon could 
      be reversed by TLR4 agonist LPS. Further, the anti-inflammatory effects of 
      loganin were attenuated when TLR4 signaling pathway was re-activated by LPS. 
      Taken together, our data indicated that loganin could attenuate inflammatory 
      response induced by Abeta in BV-2 microglia cells, partially through deactivating 
      the TLR4/TRAF6/NF-kappaB axis.
CI  - (c) 2018 International Federation for Cell Biology.
FAU - Cui, Yong
AU  - Cui Y
AD  - Department of Human Anatomy, School of Basic Medical Sciences, Liaoning 
      University of Traditional Chinese Medicine, Shenyang, 110847, People's Republic 
      of China.
FAU - Wang, Yanjie
AU  - Wang Y
AD  - Department of Biochemistry, School of Basic Medical Sciences, Liaoning University 
      of Traditional Chinese Medicine, 79 East Chongshan Road, Shenyang, 110847, 
      People's Republic of China.
FAU - Zhao, Danyu
AU  - Zhao D
AD  - Department of Biochemistry, School of Basic Medical Sciences, Liaoning University 
      of Traditional Chinese Medicine, 79 East Chongshan Road, Shenyang, 110847, 
      People's Republic of China.
FAU - Feng, Xiaofan
AU  - Feng X
AD  - Department of Biochemistry, School of Basic Medical Sciences, Liaoning University 
      of Traditional Chinese Medicine, 79 East Chongshan Road, Shenyang, 110847, 
      People's Republic of China.
FAU - Zhang, Lin
AU  - Zhang L
AD  - Department of Biochemistry, School of Basic Medical Sciences, Liaoning University 
      of Traditional Chinese Medicine, 79 East Chongshan Road, Shenyang, 110847, 
      People's Republic of China.
FAU - Liu, Chun
AU  - Liu C
AD  - Department of Biochemistry, School of Basic Medical Sciences, Liaoning University 
      of Traditional Chinese Medicine, 79 East Chongshan Road, Shenyang, 110847, 
      People's Republic of China.
LA  - eng
GR  - 20170540614/Natural Science Foundation of Liaoning Province/
PT  - Journal Article
DEP - 20181031
PL  - England
TA  - Cell Biol Int
JT  - Cell biology international
JID - 9307129
RN  - 0 (Amyloid beta-Peptides)
RN  - 0 (Iridoids)
RN  - 0 (NF-kappa B)
RN  - 0 (Peptide Fragments)
RN  - 0 (TNF Receptor-Associated Factor 6)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (amyloid beta-protein (1-42))
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - H7WJ16Q93C (loganin)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Amyloid beta-Peptides/*toxicity
MH  - Animals
MH  - Dinoprostone/biosynthesis
MH  - Inflammation/*metabolism/pathology/*prevention & control
MH  - Iridoids/*pharmacology
MH  - Mice
MH  - Microglia/*pathology
MH  - NF-kappa B/*metabolism
MH  - Nitric Oxide/biosynthesis
MH  - Peptide Fragments/*toxicity
MH  - Signal Transduction
MH  - TNF Receptor-Associated Factor 6/*metabolism
MH  - Toll-Like Receptor 4/*metabolism
OTO - NOTNLM
OT  - Alzheimer's disease
OT  - Loganin
OT  - NF-kappaB
OT  - TLR4
OT  - TRAF6
OT  - inflammation
EDAT- 2018/10/06 06:00
MHDA- 2019/01/19 06:00
CRDT- 2018/10/06 06:00
PHST- 2018/05/02 00:00 [received]
PHST- 2018/09/29 00:00 [accepted]
PHST- 2018/10/06 06:00 [pubmed]
PHST- 2019/01/19 06:00 [medline]
PHST- 2018/10/06 06:00 [entrez]
AID - 10.1002/cbin.11060 [doi]
PST - ppublish
SO  - Cell Biol Int. 2018 Dec;42(12):1632-1642. doi: 10.1002/cbin.11060. Epub 2018 Oct 
      31.