PMID- 30289808
OWN - NLM
STAT- MEDLINE
DCOM- 20191104
LR  - 20191104
IS  - 1473-5571 (Electronic)
IS  - 0269-9370 (Linking)
VI  - 32
IP  - 18
DP  - 2018 Nov 28
TI  - Inhibitory natural killer cell receptor KIR3DL1 with its ligand Bw4 constraints 
      HIV-1 disease among South Indians.
PG  - 2679-2688
LID - 10.1097/QAD.0000000000002028 [doi]
AB  - OBJECTIVE: To investigate the role of genotypic and phenotypic characteristics of 
      killer cell immunoglobulin-like receptors (KIRs) and their human leukocyte 
      antigen (HLA) class-1 ligands in HIV-1 disease progression. STUDY DESIGN AND 
      METHODS: This is a nested case-control study including 347 HIV seropositive 
      (HIV-1+) individuals from South India constituting 45 long-term nonprogressors 
      (LTNPs) and 302 disease progressors. KIR genotyping was performed by multiplex 
      sequence-specific primer-directed PCR (SSP-PCR). Phenotypic expressions of 
      KIR3DL1/S1 was studied using multiparametric flow cytometry assay. HLA-Bw4 and 
      Bw6 epitopes were determined by ARMS-PCR. HLA-Bw4I80, HLA-Bw4T80, HLA-C1, HLA-C2, 
      and HLA-Aw4 were genotyped using SSP-PCR. Serum levels of IFN-gamma was quantified 
      using ELISA method. RESULTS: Overall, 37 different KIR genotypes were observed 
      and the distribution of genotypes with AB-AB (OR = 2.2, P = 0.033) constellations 
      showed significant increase among LTNPs. The frequencies of 3DL1-2DL3-2DL5 
      (OR = 2.2, Pc = 0.031), 3DL1-Bw4/Aw4 (OR = 2.49, Pc = 0.019), homozygous Bw4 
      (OR = 2.422, Pc = 0.011) were observed higher in LTNPs and 2DS1-2DS2-2DS3 (OR =  
      0.475, Pc = 0.03), homozygous Bw6 (OR = 0.413, Pc = 0.011) were higher in the 
      disease progressors. Flow cytometry assay showed the increased expression and 
      maintenance of 3DL1/S1+NK cells in LTNPs (P = 0.0001). Further the expansion of 
      3DS1+NK cells was higher than 3DL1+NK cells in the heterozygous 3DL1/S1 LTNPs 
      (P = 0.001). CONCLUSION: The inhibitory receptor 3DL1 with Bw4 and its 
      A-haplotype defining KIR genes (2DL3/L5) confers protection against HIV-1 disease 
      progression. An increased expression and maintenance of 3DL1/S1+ natural killer 
      cells may contribute to the efficient activation of the natural killer cells and 
      subsequent long-term nonprogression (LTNPn) to the disease.
FAU - Maruthamuthu, Stalinraja
AU  - Maruthamuthu S
AD  - Department of Immunology, School of Biological Sciences, Madurai Kamaraj 
      University, Madurai.
FAU - Rajalingam, Raja
AU  - Rajalingam R
AD  - Immunogenetics and Transplantation Laboratory, Department of Surgery, University 
      of California, San Francisco, California, USA.
FAU - Pandian, Kalaimani
AU  - Pandian K
AD  - Department of Immunology, School of Biological Sciences, Madurai Kamaraj 
      University, Madurai.
FAU - Madasamy, Suresh
AU  - Madasamy S
AD  - ART Medical Centre.
FAU - Manoharan, Mythreyee
AU  - Manoharan M
AD  - Department of Microbiology, Government Theni Medical College and Hospital, Theni, 
      India.
FAU - Pitchai, Leishman
AU  - Pitchai L
AD  - Department of Immunology, School of Biological Sciences, Madurai Kamaraj 
      University, Madurai.
FAU - Murugesan, Amudhan
AU  - Murugesan A
AD  - Department of Microbiology, Government Theni Medical College and Hospital, Theni, 
      India.
FAU - Mariakuttikan, Jayalakshmi
AU  - Mariakuttikan J
AD  - Department of Immunology, School of Biological Sciences, Madurai Kamaraj 
      University, Madurai.
LA  - eng
PT  - Journal Article
PL  - England
TA  - AIDS
JT  - AIDS (London, England)
JID - 8710219
RN  - 0 (HLA-B Antigens)
RN  - 0 (KIR3DL1 protein, human)
RN  - 0 (Receptors, KIR3DL1)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Case-Control Studies
MH  - Disease Progression
MH  - Disease Resistance
MH  - Female
MH  - *Genotype
MH  - HIV Infections/*genetics/*immunology/virology
MH  - HIV-1/*immunology
MH  - HLA-B Antigens/*genetics/metabolism
MH  - Humans
MH  - India
MH  - Interferon-gamma/blood
MH  - Male
MH  - Middle Aged
MH  - Prospective Studies
MH  - Receptors, KIR3DL1/*genetics/metabolism
MH  - Young Adult
EDAT- 2018/10/06 06:00
MHDA- 2019/11/05 06:00
CRDT- 2018/10/06 06:00
PHST- 2018/10/06 06:00 [pubmed]
PHST- 2019/11/05 06:00 [medline]
PHST- 2018/10/06 06:00 [entrez]
AID - 10.1097/QAD.0000000000002028 [doi]
PST - ppublish
SO  - AIDS. 2018 Nov 28;32(18):2679-2688. doi: 10.1097/QAD.0000000000002028.