PMID- 3029121
OWN - NLM
STAT- MEDLINE
DCOM- 19870420
LR  - 20210210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 262
IP  - 8
DP  - 1987 Mar 15
TI  - Studies on the synthesis of sterol carrier protein-2 in rat adrenocortical cells 
      in monolayer culture. Regulation by ACTH and dibutyryl cyclic 3',5'-AMP.
PG  - 3713-7
AB  - The effects of ACTH or dibutyryl cyclic AMP (Bt2cAMP) on the synthesis of sterol 
      carrier protein-2 (SCP2) have been studied in rat adrenocortical cells in 
      monolayer culture. Radiolabeling of total cellular proteins with [35S]methionine 
      and immunoprecipitation with antibodies directed against rat liver SCP2, followed 
      by polyacrylamide gel electrophoresis and fluorography, showed a 3-4-fold 
      increase in the rate of synthesis of SCP2 in cells treated for 48 h with ACTH (1 
      microM) or Bt2cAMP (0.1 mM). The induction of SCP2 synthesis depended upon the 
      concentrations of ACTH or Bt2cAMP with an ED50 of 8 and 100 nM, respectively, and 
      increased linearly with time between 12 and 48 h of treatment. 
      Immunoprecipitation of SCP2 synthesized in a rabbit reticulocyte in vitro 
      translation system programmed with RNA isolated from cells treated with ACTH or 
      Bt2cAMP revealed increased synthesis of SCP2 compared to RNA from control cells. 
      The immunoprecipitable rat adrenal SCP2, synthesized in a cell-free translation 
      system, showed mobility corresponding to Mr of 14,400 upon sodium dodecyl 
      sulfate-polyacrylamide gel electrophoresis and was clearly larger than 
      immunodetectable SCP2 synthesized in cultured adrenal cells (Mr = 11,300). The 
      electrophoretic mobilities of rat liver SCP2 synthesized in cultured cells and in 
      a cell-free translation system were the same as the respective forms from rat 
      adrenal. It is concluded that the synthesis of SCP2 in rat adrenocortical cells 
      is induced by ACTH and that the induction is mediated by cAMP and may involve 
      increased levels of translatable mRNA encoding a higher molecular weight 
      precursor form of SCP2, which presumably undergoes post-translational processing 
      yielding the mature form.
FAU - Trzeciak, W H
AU  - Trzeciak WH
FAU - Simpson, E R
AU  - Simpson ER
FAU - Scallen, T J
AU  - Scallen TJ
FAU - Vahouny, G V
AU  - Vahouny GV
FAU - Waterman, M R
AU  - Waterman MR
LA  - eng
GR  - AM 10628/AM/NIADDK NIH HHS/United States
GR  - AM 28350/AM/NIADDK NIH HHS/United States
GR  - HD 13234/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Carrier Proteins)
RN  - 0 (Sterols)
RN  - 0 (Sulfur Radioisotopes)
RN  - 0 (sterol carrier proteins)
RN  - 63X7MBT2LQ (Bucladesine)
RN  - 9002-60-2 (Adrenocorticotropic Hormone)
RN  - AE28F7PNPL (Methionine)
SB  - IM
MH  - Adrenal Cortex/drug effects/*metabolism
MH  - Adrenocorticotropic Hormone/*pharmacology
MH  - Animals
MH  - Bucladesine/*pharmacology
MH  - Carrier Proteins/*biosynthesis/genetics
MH  - Cells, Cultured
MH  - Female
MH  - Kinetics
MH  - Methionine/metabolism
MH  - Protein Biosynthesis
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Sterols/*metabolism
MH  - Sulfur Radioisotopes
EDAT- 1987/03/15 00:00
MHDA- 1987/03/15 00:01
CRDT- 1987/03/15 00:00
PHST- 1987/03/15 00:00 [pubmed]
PHST- 1987/03/15 00:01 [medline]
PHST- 1987/03/15 00:00 [entrez]
AID - S0021-9258(18)61413-9 [pii]
PST - ppublish
SO  - J Biol Chem. 1987 Mar 15;262(8):3713-7.