PMID- 30292447
OWN - NLM
STAT- MEDLINE
DCOM- 20190625
LR  - 20190625
IS  - 1578-1267 (Electronic)
IS  - 0301-0546 (Linking)
VI  - 47
IP  - 2
DP  - 2019 Mar-Apr
TI  - Osteoprotegerin mediate RANK/RANKL signaling inhibition eases asthma inflammatory 
      reaction by affecting the survival and function of dendritic cells.
PG  - 179-184
LID - S0301-0546(18)30117-4 [pii]
LID - 10.1016/j.aller.2018.06.006 [doi]
AB  - INTRODUCTION: Asthma is a chronic inflammatory, heterogeneous airway disease 
      affecting millions of people around the world. Dendritic cells (DCs) are 
      considered the most important antigen-presenting cell in asthma airway 
      inflammatory reaction. But whether osteoprotegerin (OPG) mediate RANK/RANKL 
      signaling inhibition influences asthma development by affecting the survival and 
      function of DCs remains unclear. In this study, we assessed the effects of OPG on 
      DCs and asthma. MATERIAL AND METHODS: BALB/c mice immunized with ovalbumin (OVA) 
      were challenged thrice with an aerosol of OVA every second day for eight days. 
      Dexamethasone (1.0mg/kg) or OPG (50mug/kg) was administered intraperitoneally to 
      OVA-immunized BALB/c mice on day 24 once a day for nine days. Mice were analyzed 
      for effects of OPG on asthma, inflammatory cell infiltration and cytokine levels 
      in lung tissue. The expression of RANK and beta-actin was detected by Western Blot. 
      DCs were isolated from mouse bone morrow. Cell survival was assessed by cell 
      counting. The content of IL-12 was detected by ELISA. RESULTS: Results showed 
      that OVA increased the number of inflammatory factors in BALF, elevated lung 
      inflammation scores in mice. OPG reversed the alterations induced by OVA in the 
      asthmatic mice. OPG inhibited the survival and function of DC via inhibition of 
      RANK/RANKL signaling. CONCLUSIONS: This research proved inhibition of RANK/RANKL 
      signaling by OPG could ease the inflammatory reaction in asthma, providing new 
      evidence for the application of OPG on asthma.
CI  - Copyright (c) 2018. Published by Elsevier Espana, S.L.U.
FAU - Yang, X
AU  - Yang X
AD  - Department of Respiratory Medicine, The Second Affiliated Hospital of Xi'an 
      Jiaotong University, Xi'an, China.
FAU - Wang, X
AU  - Wang X
AD  - Department of Respiratory Medicine, The Second Affiliated Hospital of Xi'an 
      Jiaotong University, Xi'an, China.
FAU - Chi, M
AU  - Chi M
AD  - Department of Pediatrics, BaYi Children's Hospital of the PLA Army General 
      Hospital, Beijing, China; The Clinical Medical College of the PLA Army, Second 
      Military Medical University of People's Liberation Army, Shanghai, China.
FAU - Zhang, M
AU  - Zhang M
AD  - Department of Respiratory Medicine, The Second Affiliated Hospital of Xi'an 
      Jiaotong University, Xi'an, China.
FAU - Shan, H
AU  - Shan H
AD  - Department of Respiratory Medicine, The Second Affiliated Hospital of Xi'an 
      Jiaotong University, Xi'an, China.
FAU - Zhang, Q-H
AU  - Zhang QH
AD  - Department of Respiratory Medicine, The Second Affiliated Hospital of Xi'an 
      Jiaotong University, Xi'an, China.
FAU - Zhang, J
AU  - Zhang J
AD  - Department of Respiratory Medicine, The Second Affiliated Hospital of Xi'an 
      Jiaotong University, Xi'an, China.
FAU - Shi, J
AU  - Shi J
AD  - Department of Respiratory Medicine, The Second Affiliated Hospital of Xi'an 
      Jiaotong University, Xi'an, China.
FAU - Zhang, J-Z
AU  - Zhang JZ
AD  - Department of Respiratory Medicine, The First Affiliated Hospital of Xi'an 
      Jiaotong University, Xi'an, China.
FAU - Wu, R-M
AU  - Wu RM
AD  - Department of Respiratory Medicine, Shaanxi Provincial People's Hospital, Xi'an, 
      China.
FAU - Li, Y-L
AU  - Li YL
AD  - Department of Respiratory Medicine, The Second Affiliated Hospital of Xi'an 
      Jiaotong University, Xi'an, China. Electronic address: yalili2008@163.com.
LA  - eng
PT  - Journal Article
DEP - 20181003
PL  - Singapore
TA  - Allergol Immunopathol (Madr)
JT  - Allergologia et immunopathologia
JID - 0370073
RN  - 0 (Cytokines)
RN  - 0 (Osteoprotegerin)
RN  - 0 (RANK Ligand)
RN  - 0 (Receptor Activator of Nuclear Factor-kappa B)
SB  - IM
MH  - Animals
MH  - Antigen Presentation
MH  - Asthma/immunology/*metabolism
MH  - Cell Survival
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*physiology
MH  - Female
MH  - Humans
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Osteoprotegerin/*metabolism
MH  - Pneumonia/immunology/*metabolism
MH  - RANK Ligand/metabolism
MH  - Receptor Activator of Nuclear Factor-kappa B/metabolism
MH  - Signal Transduction
OTO - NOTNLM
OT  - Asthma
OT  - Dendritic cells
OT  - Osteoprotegerin
OT  - RANK/RANKL
EDAT- 2018/10/08 06:00
MHDA- 2019/06/27 06:00
CRDT- 2018/10/08 06:00
PHST- 2018/04/02 00:00 [received]
PHST- 2018/05/22 00:00 [revised]
PHST- 2018/06/13 00:00 [accepted]
PHST- 2018/10/08 06:00 [pubmed]
PHST- 2019/06/27 06:00 [medline]
PHST- 2018/10/08 06:00 [entrez]
AID - S0301-0546(18)30117-4 [pii]
AID - 10.1016/j.aller.2018.06.006 [doi]
PST - ppublish
SO  - Allergol Immunopathol (Madr). 2019 Mar-Apr;47(2):179-184. doi: 
      10.1016/j.aller.2018.06.006. Epub 2018 Oct 3.