PMID- 30293237 OWN - NLM STAT- MEDLINE DCOM- 20190520 LR - 20200225 IS - 1751-7915 (Electronic) IS - 1751-7915 (Linking) VI - 12 IP - 2 DP - 2019 Mar TI - In-situ microbially induced Ca(2+) -alginate polymeric sealant for seepage control in porous materials. PG - 324-333 LID - 10.1111/1751-7915.13315 [doi] AB - This paper presents a novel approach of using in-situ microbially induced Ca(2+) -alginate polymeric sealant for seepage control in porous materials. This process comprises two steps: (i) generation of insoluble calcium carbonate inside the pores of porous materials (such as sand) through a microbially induced carbonate precipitation (MICP) process in-situ and (ii) injection of sodium alginate for in-situ gelation via reaction between alginate and Ca(2+) ions. The experimental results showed that the hydraulic conductivity/permeability of sand decreased with the increase in alginate concentration. When 5% alginate was used with a CaCO(3) concentration of 0.18 g g(-1) sand, the permeability of the alginate-treated sand reduced from 5.0 x 10(-4) to 2.2 x 10(-9) m s(-1) . The scanning electron microscopy images revealed that a film-type coating was formed around sand particles with spherical round crystals embedded. Furthermore, the in-situ formed Ca-alginate polymeric sealant can also be used for the removal of Cu(2+) ion and suspended particles from contaminated water by more than 90%. Built on the current research, the envisioned practical application of the proposed method may include clogging fractured rock, reducing seepage and prevent piping through dams, excavation dewatering, and forming barriers for remediating specific contaminants. CI - (c) 2018 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. FAU - Cheng, Liang AU - Cheng L AUID- ORCID: 0000-0002-1767-2108 AD - Department of Civil and Environmental Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore City, 639798, Singapore. FAU - Yang, Yang AU - Yang Y AD - Department of Civil and Environmental Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore City, 639798, Singapore. FAU - Chu, Jian AU - Chu J AD - Department of Civil and Environmental Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore City, 639798, Singapore. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20181006 PL - United States TA - Microb Biotechnol JT - Microbial biotechnology JID - 101316335 RN - 0 (Alginates) RN - 0 (Gels) RN - H0G9379FGK (Calcium Carbonate) SB - IM MH - Alginates/*metabolism MH - Biotechnology/*methods MH - Calcium Carbonate/*metabolism MH - Gels/*metabolism MH - Microscopy, Electron, Scanning PMC - PMC6389844 COIS- None declared. EDAT- 2018/10/08 06:00 MHDA- 2019/05/21 06:00 PMCR- 2018/10/06 CRDT- 2018/10/08 06:00 PHST- 2018/05/01 00:00 [received] PHST- 2018/08/09 00:00 [revised] PHST- 2018/08/27 00:00 [accepted] PHST- 2018/10/08 06:00 [pubmed] PHST- 2019/05/21 06:00 [medline] PHST- 2018/10/08 06:00 [entrez] PHST- 2018/10/06 00:00 [pmc-release] AID - MBT213315 [pii] AID - 10.1111/1751-7915.13315 [doi] PST - ppublish SO - Microb Biotechnol. 2019 Mar;12(2):324-333. doi: 10.1111/1751-7915.13315. Epub 2018 Oct 6.