PMID- 30293648
OWN - NLM
STAT- MEDLINE
DCOM- 20190130
LR  - 20190130
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 225
DP  - 2018 Nov
TI  - Use of processing fluids and serum samples to characterize porcine reproductive 
      and respiratory syndrome virus dynamics in 3 day-old pigs.
PG  - 149-156
LID - S0378-1135(18)30834-4 [pii]
LID - 10.1016/j.vetmic.2018.09.006 [doi]
AB  - Collection of serum samples of pigs at weaning to monitor for porcine 
      reproductive and respiratory syndrome virus (PRRSV) has become a common practice 
      to determine PRRSV herd infection status. Diagnostic sensitivity of this practice 
      is low in herds undergoing PRRSV elimination once prevalence of infection is near 
      zero. Thus, the goal of this study was to characterize the dynamics of PRRSV 
      infection in 3 day-old pigs overtime using serum and serosanguineous fluids 
      obtained as part of castration and tail docking practices (processing fluids 
      (PF)). Secondary goal was to estimate sensitivity and specificity of PF in the 3 
      day old population. A 6000 breed-to-wean sow herd was monitored every three weeks 
      for 23 weeks after a PRRSV outbreak by collecting both PF and individual serum 
      samples from all pigs in the selected litters. Out of the 77 litters tested, 23 
      (29.8%) were identified as positive using the PF and the serum samples, with a 
      Cohen's kappa statistic of 0.81 (95% CI: 0.59-1) between the results obtained in 
      each sample type. The sensitivity and specificity of the PF relative to the 
      results in serum was 87% (95% CI: 66%-97%) and 94% (95% CI: 85%-99%) 
      respectively. The percentage of PRRSV positive litters decreased over time and 
      litters from gilts were more likely to test positive than those from older sows. 
      Overall, the study demonstrates that PF can be a convenient and reliable specimen 
      to monitor PRRSV infection in breeding herds.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Vilalta, Carles
AU  - Vilalta C
AD  - Veterinary Population Medicine Department, College of Veterinary Medicine, 
      University of Minnesota, 1365 Gortner Ave., St Paul, MN 55108, United States. 
      Electronic address: cvilalta@umn.edu.
FAU - Sanhueza, Juan
AU  - Sanhueza J
AD  - Veterinary Population Medicine Department, College of Veterinary Medicine, 
      University of Minnesota, 1365 Gortner Ave., St Paul, MN 55108, United States.
FAU - Alvarez, Julio
AU  - Alvarez J
AD  - VISAVET Health Surveillance Center, Universidad Complutense, Avda Puerta de 
      Hierro S/N, Madrid, 28040, Spain; Departamento de Sanidad Animal, Facultad de 
      Veterinaria, Universidad Complutense, Avda Puerta de Hierro S/N, Madrid, 28040, 
      Spain.
FAU - Murray, Deb
AU  - Murray D
AD  - New Fashion Pork, Jackson, MN, United States.
FAU - Torremorell, Montserrat
AU  - Torremorell M
AD  - Veterinary Population Medicine Department, College of Veterinary Medicine, 
      University of Minnesota, 1365 Gortner Ave., St Paul, MN 55108, United States.
FAU - Corzo, Cesar
AU  - Corzo C
AD  - Veterinary Population Medicine Department, College of Veterinary Medicine, 
      University of Minnesota, 1365 Gortner Ave., St Paul, MN 55108, United States.
FAU - Morrison, Robert
AU  - Morrison R
AD  - Veterinary Population Medicine Department, College of Veterinary Medicine, 
      University of Minnesota, 1365 Gortner Ave., St Paul, MN 55108, United States.
LA  - eng
PT  - Journal Article
DEP - 20180917
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Antibodies, Viral/blood
MH  - Body Fluids/*virology
MH  - Breeding
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Feces/virology
MH  - Female
MH  - Porcine Reproductive and Respiratory 
      Syndrome/*blood/*diagnosis/epidemiology/virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*isolation & 
      purification
MH  - Prevalence
MH  - Saliva/virology
MH  - Sensitivity and Specificity
MH  - Serum/*virology
MH  - Swine
MH  - United States/epidemiology
MH  - Weaning
OTO - NOTNLM
OT  - Monitoring
OT  - PRRSV
OT  - Processing fluids
EDAT- 2018/10/09 06:00
MHDA- 2019/01/31 06:00
CRDT- 2018/10/09 06:00
PHST- 2018/07/10 00:00 [received]
PHST- 2018/09/12 00:00 [revised]
PHST- 2018/09/13 00:00 [accepted]
PHST- 2018/10/09 06:00 [pubmed]
PHST- 2019/01/31 06:00 [medline]
PHST- 2018/10/09 06:00 [entrez]
AID - S0378-1135(18)30834-4 [pii]
AID - 10.1016/j.vetmic.2018.09.006 [doi]
PST - ppublish
SO  - Vet Microbiol. 2018 Nov;225:149-156. doi: 10.1016/j.vetmic.2018.09.006. Epub 2018 
      Sep 17.