PMID- 30296567
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20181211
IS  - 1879-0038 (Electronic)
IS  - 0378-1119 (Linking)
VI  - 682
DP  - 2019 Jan 15
TI  - Chicken GHR antisense transcript regulates its sense transcript in hepatocytes.
PG  - 101-110
LID - S0378-1119(18)31027-8 [pii]
LID - 10.1016/j.gene.2018.10.001 [doi]
AB  - An increasing number of evidences indicated that long noncoding RNAs (LncRNAs) 
      regulate a variety of biological progresses via different mechanisms. Our 
      previous study had identified a chicken growth hormone receptor (GHR) antisense 
      transcript (GHR-AS) which regulated GHR sense transcript (GHR-S) in LMH cells. In 
      the present study, roles of GHR-AS and its regulatory mechanism were analyzed in 
      chicken hepatocytes. The expression patterns of liver GHR-S, GHR-AS and Let-7b 
      ascended with the development of chicken. The hepatocytes proliferation was 
      promoted and more cells entered into DNA synthesis (S) phase when GHR-AS was 
      overexpressed while the cell proliferation was slowed and fewer cells were in S 
      phase when GHR-AS was interfered. Meanwhile, the GHR-S increased when we 
      overexpressed GHR-AS while it reduced when GHR-AS was inhibited. The S1 Nuclease 
      protection assay indicated that GHR-S and GHR-AS formed RNA duplex via GHR-S 3' 
      untranslation regon (3'UTR). In hepatocytes or LMH cells, the half-time of GHR-S 
      showed a delayed trend when GHR-AS or GHR-AS 5' untranslation regon (5'UTR) was 
      overexpressed. Furthermore, the level of GHR-S can be decreased by Let-7b mimics 
      whereas it was partially rescued when co-transfected pGHR-AS or pGHR-AS 5'UTR 
      with Let-7b mimics. Based on our findings, GHR-AS affected hepatocytes 
      proliferation and improved GHR-S stability possibly by forming RNA duplex between 
      GHR-S and GHR-AS, competing with Let-7b.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Wang, Zhang
AU  - Wang Z
AD  - Agricultural College, GuangDong Ocean University, ZhanJiang, China.
FAU - Xu, HaiDong
AU  - Xu H
AD  - Agricultural College, GuangDong Ocean University, ZhanJiang, China.
FAU - Li, Ting
AU  - Li T
AD  - Agricultural College, GuangDong Ocean University, ZhanJiang, China.
FAU - Wu, Jiang
AU  - Wu J
AD  - Agricultural College, GuangDong Ocean University, ZhanJiang, China.
FAU - An, LiLong
AU  - An L
AD  - Agricultural College, GuangDong Ocean University, ZhanJiang, China.
FAU - Zhao, ZhiHui
AU  - Zhao Z
AD  - Agricultural College, GuangDong Ocean University, ZhanJiang, China.
FAU - Xiao, Mei
AU  - Xiao M
AD  - Agricultural College, GuangDong Ocean University, ZhanJiang, China.
FAU - Adu-Asiamah, Patricia
AU  - Adu-Asiamah P
AD  - Agricultural College, GuangDong Ocean University, ZhanJiang, China.
FAU - Zhang, XiQuan
AU  - Zhang X
AD  - Guangdong Provincial Key Lab of Agro-animal Genomics and Molecular Breeding and 
      Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, 
      South China Agricultural University, Guangzhou, China.
FAU - Zhang, Li
AU  - Zhang L
AD  - Agricultural College, GuangDong Ocean University, ZhanJiang, China. Electronic 
      address: WschoolZ@163.com.
LA  - eng
PT  - Journal Article
DEP - 20181005
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (3' Untranslated Regions)
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Oligonucleotides, Antisense)
RN  - 0 (Receptors, Somatotropin)
SB  - IM
MH  - 3' Untranslated Regions
MH  - 5' Untranslated Regions
MH  - Animals
MH  - Cell Proliferation
MH  - Chick Embryo
MH  - Chickens/genetics
MH  - Gene Expression Profiling
MH  - *Gene Expression Regulation
MH  - Hepatocytes/cytology/*metabolism
MH  - Liver/metabolism
MH  - Oligonucleotides, Antisense/*genetics
MH  - Receptors, Somatotropin/*genetics
MH  - Transfection
OTO - NOTNLM
OT  - Double strand RNA
OT  - Growth hormone receptor
OT  - Natural antisense transcript
OT  - Stability
EDAT- 2018/10/09 06:00
MHDA- 2018/12/12 06:00
CRDT- 2018/10/09 06:00
PHST- 2018/04/28 00:00 [received]
PHST- 2018/07/29 00:00 [revised]
PHST- 2018/10/01 00:00 [accepted]
PHST- 2018/10/09 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
PHST- 2018/10/09 06:00 [entrez]
AID - S0378-1119(18)31027-8 [pii]
AID - 10.1016/j.gene.2018.10.001 [doi]
PST - ppublish
SO  - Gene. 2019 Jan 15;682:101-110. doi: 10.1016/j.gene.2018.10.001. Epub 2018 Oct 5.