PMID- 30297106
OWN - NLM
STAT- MEDLINE
DCOM- 20190513
LR  - 20190520
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 505
IP  - 3
DP  - 2018 Nov 2
TI  - Gene expression profiling in adipose tissue of Sprague Dawley rats identifies 
      olfactory receptor 984 as a potential obesity treatment target.
PG  - 801-806
LID - S0006-291X(18)32071-0 [pii]
LID - 10.1016/j.bbrc.2018.09.137 [doi]
AB  - The aim of the study was to identify and functionally characterize novel 
      candidate gene/s involved in the development of resistance to diet-induced 
      obesity in rats. In a high-fat-diet (HFD) study of rats, we found subgroups which 
      either developed resistance to HFD-induced obesity (DR) or showed an 
      obesity-prone phenotype (DIO). Gene expression analysis in 10 samples (5 DIO vs 5 
      DR) was performed. The most promising gene, OR6C3 (orthologous with rat Olr984 
      and mouse Olfr788) was measured by qRT-PCR in paired samples of human visceral 
      (Vis) and subcutaneous (SC) adipose tissue (AT) (n = 225) and in sub-fractions of 
      adipocytes and cells of stromal vascular fraction. Gene expression analyses 
      showed Olr984 with significantly reduced mRNA expression in DR rats. In the Vis 
      AT of human samples we found an up-regulation of OR6C3 compared to SC AT, 
      independent of gender, glucose tolerance or type 2 diabetes. We observed 
      significantly lower levels of SC AT OR6C3 mRNA in subjects with obesity compared 
      to those with normal-weight or overweight. OR6C3 is more expressed in SVF than in 
      adipocytes. Olr984 could be a novel candidate gene related to diet-induced 
      obesity in rats. Variation in human AT mRNA expression is related to obesity 
      parameters and glucose homeostasis and linked to the regulatory role of insulin 
      on the Olr984.
CI  - Copyright (c) 2018 Elsevier Inc. All rights reserved.
FAU - Giusepponi, Maria Elena
AU  - Giusepponi ME
AD  - Department of Medicine, University of Leipzig, 04103, Leipzig, Germany; 
      Pharmacology Unit, School of Pharmacy, University of Camerino, Camerino, Italy.
FAU - Kern, Matthias
AU  - Kern M
AD  - Department of Medicine, University of Leipzig, 04103, Leipzig, Germany.
FAU - Chakaroun, Rima
AU  - Chakaroun R
AD  - Department of Medicine, University of Leipzig, 04103, Leipzig, Germany; Leipzig 
      University Medical Center, IFB AdiposityDiseases, University of Leipzig, 04103, 
      Leipzig, Germany.
FAU - Wohland, Tobias
AU  - Wohland T
AD  - Department of Medicine, University of Leipzig, 04103, Leipzig, Germany; Leipzig 
      University Medical Center, IFB AdiposityDiseases, University of Leipzig, 04103, 
      Leipzig, Germany.
FAU - Kovacs, Peter
AU  - Kovacs P
AD  - Department of Medicine, University of Leipzig, 04103, Leipzig, Germany; Leipzig 
      University Medical Center, IFB AdiposityDiseases, University of Leipzig, 04103, 
      Leipzig, Germany.
FAU - Dietrich, Arne
AU  - Dietrich A
AD  - Department of Surgery, University of Leipzig, Leipzig, Germany.
FAU - Schon, Michael R
AU  - Schon MR
AD  - Clinic of Visceral Surgery, Stadtisches Klinikum Karlsruhe, Karlsruhe, Germany.
FAU - Krohn, Knut
AU  - Krohn K
AD  - Core Unit IZKF, University of Leipzig, Leipzig, Germany.
FAU - Pucci, Mariangela
AU  - Pucci M
AD  - Faculty of Bioscience and Technology for Food, Agriculture and Environment, 
      University of Teramo, Italy.
FAU - Polidori, Carlo
AU  - Polidori C
AD  - Pharmacology Unit, School of Pharmacy, University of Camerino, Camerino, Italy.
FAU - Micioni Di Bonaventura, Maria Vittoria
AU  - Micioni Di Bonaventura MV
AD  - Pharmacology Unit, School of Pharmacy, University of Camerino, Camerino, Italy.
FAU - Stumvoll, Michael
AU  - Stumvoll M
AD  - Department of Medicine, University of Leipzig, 04103, Leipzig, Germany; Leipzig 
      University Medical Center, IFB AdiposityDiseases, University of Leipzig, 04103, 
      Leipzig, Germany.
FAU - Bluher, Matthias
AU  - Bluher M
AD  - Department of Medicine, University of Leipzig, 04103, Leipzig, Germany; Leipzig 
      University Medical Center, IFB AdiposityDiseases, University of Leipzig, 04103, 
      Leipzig, Germany.
FAU - Cifani, Carlo
AU  - Cifani C
AD  - Pharmacology Unit, School of Pharmacy, University of Camerino, Camerino, Italy.
FAU - Kloting, Nora
AU  - Kloting N
AD  - Department of Medicine, University of Leipzig, 04103, Leipzig, Germany; Leipzig 
      University Medical Center, IFB AdiposityDiseases, University of Leipzig, 04103, 
      Leipzig, Germany. Electronic address: nora.kloeting@medizin.uni-leipzig.de.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181005
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Olr984 protein, rat)
RN  - 0 (Receptors, Odorant)
SB  - IM
MH  - Adipose Tissue/*metabolism
MH  - Animals
MH  - Diet, High-Fat/adverse effects
MH  - *Gene Expression Profiling
MH  - Humans
MH  - Insulin Resistance
MH  - Mice
MH  - Obesity/*drug therapy/etiology/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Odorant/*genetics
MH  - Subcutaneous Fat
OTO - NOTNLM
OT  - Adipose tissue
OT  - Insulin sensitivity
OT  - OR6C3
OT  - Obesity resistance
OT  - Olr984
EDAT- 2018/10/10 06:00
MHDA- 2019/05/14 06:00
CRDT- 2018/10/10 06:00
PHST- 2018/09/14 00:00 [received]
PHST- 2018/09/21 00:00 [accepted]
PHST- 2018/10/10 06:00 [pubmed]
PHST- 2019/05/14 06:00 [medline]
PHST- 2018/10/10 06:00 [entrez]
AID - S0006-291X(18)32071-0 [pii]
AID - 10.1016/j.bbrc.2018.09.137 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2018 Nov 2;505(3):801-806. doi: 
      10.1016/j.bbrc.2018.09.137. Epub 2018 Oct 5.