PMID- 30298249
OWN - NLM
STAT- MEDLINE
DCOM- 20190704
LR  - 20190704
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1849
DP  - 2018
TI  - Quantification of Vitamin B(12)-Related Proteins in Marine Microbial Systems 
      Using Selected Reaction Monitoring Mass Spectrometry.
PG  - 87-98
LID - 10.1007/978-1-4939-8728-3_6 [doi]
AB  - Mass spectrometry-based proteomic approaches to studying microbial systems enable 
      assessment of taxonomically resolved functional capacity. A subset of these 
      proteomic approaches are absolutely quantitative, enabling comparisons of protein 
      expression patterns between different studies and across environments. This 
      chapter outlines a method for applying quantitative assays in marine microbial 
      communities, using proteins involved in vitamin B(12) (cobalamin) utilization and 
      production as specific examples. This approach involves identifying important 
      protein targets, determining taxonomic resolution of the required assays, 
      identifying suitable peptides, developing and optimizing liquid 
      chromatography-selected reaction monitoring mass spectrometry assays (LC-SRM-MS), 
      and processing the resulting data. Implementing the method outlined here results 
      in measurements (fmol diagnostic peptide per mug of total bulk protein) that, in 
      this case, define the nutritional status of microbial community members with 
      respect to vitamin B(12), and are comparable across and between marine microbial 
      systems.
FAU - Bertrand, Erin M
AU  - Bertrand EM
AD  - Department of Biology, Dalhousie University, Halifax, NS, Canada. 
      erin.bertrand@dal.ca.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Bacterial Proteins)
RN  - 0 (Peptide Fragments)
RN  - P6YC3EG204 (Vitamin B 12)
SB  - IM
MH  - Bacteria/*metabolism
MH  - Bacterial Proteins/*metabolism
MH  - *Biomass
MH  - Mass Spectrometry/*methods
MH  - Peptide Fragments/*analysis/metabolism
MH  - Seawater/*microbiology
MH  - Vitamin B 12/*metabolism
MH  - Water Microbiology
OTO - NOTNLM
OT  - Mass spectrometry
OT  - Microbial systems
OT  - Selected reaction monitoring
OT  - Targeted metaproteomics
EDAT- 2018/10/10 06:00
MHDA- 2019/07/05 06:00
CRDT- 2018/10/10 06:00
PHST- 2018/10/10 06:00 [entrez]
PHST- 2018/10/10 06:00 [pubmed]
PHST- 2019/07/05 06:00 [medline]
AID - 10.1007/978-1-4939-8728-3_6 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2018;1849:87-98. doi: 10.1007/978-1-4939-8728-3_6.