PMID- 30298565
OWN - NLM
STAT- MEDLINE
DCOM- 20190404
LR  - 20220720
IS  - 1528-1167 (Electronic)
IS  - 0013-9580 (Print)
IS  - 0013-9580 (Linking)
VI  - 59
IP  - 11
DP  - 2018 Nov
TI  - Effects of galanin receptor 2 and receptor 3 knockout in mouse models of acute 
      seizures.
PG  - e166-e171
LID - 10.1111/epi.14573 [doi]
AB  - There exists solid evidence that endogenous galanin and galanin agonists exert 
      anticonvulsive actions mediated both by galanin 1 receptor (GAL1-R) and galanin 2 
      receptor (GAL2-R). We have now investigated whether depletion of the recently 
      identified third galanin receptor, GAL3-R, and that of GAL2-R, alters the 
      threshold to the systemically applied gamma-aminobutyric acid (GABA) antagonist 
      pentylenetetrazole (PTZ) or to intrahippocampally administered kainic acid (KA). 
      In neither model, GAL3-KO mice differed in their latency to the first seizure, 
      mean seizure duration, total number of seizures, or time spent in seizures 
      compared to wild-type controls. In addition, consistent with previous data, the 
      response to PTZ was not altered in GAL2-KO mice. In contrast, intrahippocampal KA 
      resulted in a significantly increased number of seizures and time spent in 
      seizures in GAL2-KO mice, although the latency to the first seizure and the 
      duration of individual seizures was not altered. These results are consistent 
      with the previous data showing that GAL2-R knockdown does not affect the number 
      of perforant path stimulations required for initiating status epilepticus but 
      significantly increases the seizure severity during the ongoing status. In 
      conclusion, our data support a specific role of GAL2-R but not of GAL3-R in 
      mediating the anticonvulsive actions of endogenous galanin.
CI  - (c) The Authors. Epilepsia published by Wiley Periodicals, Inc. on behalf of 
      International League Against Epilepsy.
FAU - Drexel, Meinrad
AU  - Drexel M
AUID- ORCID: 0000-0002-2705-9673
AD  - Department of Pharmacology, Medical University of Innsbruck, Innsbruck, Austria.
FAU - Sternberg', Felix
AU  - Sternberg' F
AD  - Laura Bassi Centre of Expertise-THERAPEP, Research Program for Receptor 
      Biochemistry and Tumor Metabolism, Department of Pediatrics, Paracelsus Medical 
      University, Salzburg, Austria.
FAU - Kofler, Barbara
AU  - Kofler B
AD  - Laura Bassi Centre of Expertise-THERAPEP, Research Program for Receptor 
      Biochemistry and Tumor Metabolism, Department of Pediatrics, Paracelsus Medical 
      University, Salzburg, Austria.
FAU - Sperk, Gunther
AU  - Sperk G
AD  - Department of Pharmacology, Medical University of Innsbruck, Innsbruck, Austria.
LA  - eng
SI  - GENBANK/TSP075150
GR  - P 26680/Austrian Science Fund FWF/Austria
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181008
PL  - United States
TA  - Epilepsia
JT  - Epilepsia
JID - 2983306R
RN  - 0 (Receptor, Galanin, Type 2)
RN  - 0 (Receptor, Galanin, Type 3)
RN  - SIV03811UC (Kainic Acid)
RN  - WM5Z385K7T (Pentylenetetrazole)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Electroencephalography
MH  - Hippocampus/drug effects
MH  - Kainic Acid/toxicity
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Pentylenetetrazole/toxicity
MH  - Reaction Time/drug effects/genetics
MH  - Receptor, Galanin, Type 2/*deficiency/genetics
MH  - Receptor, Galanin, Type 3/*deficiency/genetics
MH  - Seizures/chemically induced/*genetics
PMC - PMC6282553
OTO - NOTNLM
OT  - GAL2-R
OT  - GAL3-R
OT  - hippocampus
OT  - kainic acid
OT  - pentylenetetrazole
OT  - seizure protection
EDAT- 2018/10/10 06:00
MHDA- 2019/04/05 06:00
PMCR- 2018/12/06
CRDT- 2018/10/10 06:00
PHST- 2018/08/20 00:00 [received]
PHST- 2018/09/07 00:00 [revised]
PHST- 2018/09/10 00:00 [accepted]
PHST- 2018/10/10 06:00 [pubmed]
PHST- 2019/04/05 06:00 [medline]
PHST- 2018/10/10 06:00 [entrez]
PHST- 2018/12/06 00:00 [pmc-release]
AID - EPI14573 [pii]
AID - 10.1111/epi.14573 [doi]
PST - ppublish
SO  - Epilepsia. 2018 Nov;59(11):e166-e171. doi: 10.1111/epi.14573. Epub 2018 Oct 8.