PMID- 30301390
OWN - NLM
STAT- MEDLINE
DCOM- 20190109
LR  - 20190109
IS  - 1744-5116 (Electronic)
IS  - 1388-0209 (Print)
IS  - 1388-0209 (Linking)
VI  - 56
IP  - 1
DP  - 2018 Dec
TI  - Dicranopteris linearis extract inhibits the proliferation of human breast cancer 
      cell line (MDA-MB-231) via induction of S-phase arrest and apoptosis.
PG  - 422-432
LID - 10.1080/13880209.2018.1495748 [doi]
AB  - CONTEXT: Dicranopteris linearis (Burm.f.) Underw. (Gleicheniaceae) has been 
      scientifically proven to exert various pharmacological activities. Nevertheless, 
      its anti-proliferative potential has not been extensively investigated. 
      OBJECTIVE: To investigate the anti-proliferative potential of D. linearis leaves 
      and determine possible mechanistic pathways. MATERIALS AND METHODS: MTT assay was 
      used to determine the cytotoxic effects of D. linearis methanol (MEDL) and 
      petroleum ether (PEEDL) extracts at concentrations of 100, 50, 25, 12.5, 6.25 and 
      3.125 microg/mL against a panel of cancer cell lines (breast [MCF-7 and MDA-MB-231], 
      cervical [HeLa], colon [HT-29], hepatocellular [HepG2] and lung [A549]), as 
      compared to negative (untreated) and positive [5-fluorouracil (5-FU)-treated] 
      control groups. Mouse fibroblast cells (3T3) were used as normal cells. The mode 
      of cell death was examined using morphological analysis via acridine orange (AO) 
      and propidium iodide (PI) double staining. Cell cycle arrest was determined using 
      flow cytometer, followed by annexin V-PI apoptosis detection kit. RESULTS: MEDL 
      demonstrated the most significant growth inhibition against MDA-MB-231 cells 
      (IC(50) 22.4 microg/mL). PEEDL showed no cytotoxic effect. Induction of apoptosis by 
      MEDL was evidenced via morphological analysis and acridine orange propidium 
      iodide staining. MEDL could induce S phase cell cycle arrest after 72 h of 
      incubation. Early apoptosis induction in MDA-MB-231 cells was confirmed by 
      annexin V-FITC and PI staining. Significant increase in apoptotic cells were 
      detected after 24 h of treatment with 15.07% cells underwent apoptosis, and the 
      amount escalated to 18.24% with prolonged 48 h incubation. CONCLUSIONS: MEDL has 
      potential as a potent cytotoxic agent against MDA-MB-231 adenocarcinoma.
FAU - Baharuddin, Aifaa Akmal
AU  - Baharuddin AA
AD  - a Halal Products Development, Halal Products Research Institute , Universiti 
      Putra Malaysia (UPM) Serdang , Selangor , Malaysia.
FAU - Roosli, Rushduddin Al Jufri
AU  - Roosli RAJ
AD  - b Department of Biomedical Science, Faculty of Medicine and Health Sciences , 
      Universiti Putra Malaysia (UPM) Serdang , Selangor , Malaysia.
FAU - Zakaria, Zainul Amiruddin
AU  - Zakaria ZA
AUID- ORCID: 0000-0001-5525-7821
AD  - b Department of Biomedical Science, Faculty of Medicine and Health Sciences , 
      Universiti Putra Malaysia (UPM) Serdang , Selangor , Malaysia.
FAU - Md Tohid, Siti Farah
AU  - Md Tohid SF
AUID- ORCID: 0000-0002-4772-1062
AD  - a Halal Products Development, Halal Products Research Institute , Universiti 
      Putra Malaysia (UPM) Serdang , Selangor , Malaysia.
AD  - b Department of Biomedical Science, Faculty of Medicine and Health Sciences , 
      Universiti Putra Malaysia (UPM) Serdang , Selangor , Malaysia.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Pharm Biol
JT  - Pharmaceutical biology
JID - 9812552
RN  - 0 (Plant Extracts)
SB  - IM
MH  - 3T3 Cells
MH  - A549 Cells
MH  - Animals
MH  - Apiaceae
MH  - Apoptosis/*drug effects/physiology
MH  - Breast Neoplasms/drug therapy/*pathology
MH  - Cell Cycle Checkpoints/*drug effects/physiology
MH  - Cell Line, Tumor
MH  - Cell Proliferation/*drug effects/physiology
MH  - HeLa Cells
MH  - Hep G2 Cells
MH  - Humans
MH  - MCF-7 Cells
MH  - Mice
MH  - Plant Extracts/isolation & purification/*pharmacology
MH  - S Phase Cell Cycle Checkpoints/*drug effects/physiology
PMC - PMC6179048
OTO - NOTNLM
OT  - Anticancer
OT  - acridine orange propidium iodide
OT  - annexin-V FITC
OT  - antiproliferative
OT  - cell cycle arrest
EDAT- 2018/10/12 06:00
MHDA- 2019/01/10 06:00
PMCR- 2018/10/10
CRDT- 2018/10/11 06:00
PHST- 2018/10/11 06:00 [entrez]
PHST- 2018/10/12 06:00 [pubmed]
PHST- 2019/01/10 06:00 [medline]
PHST- 2018/10/10 00:00 [pmc-release]
AID - 1495748 [pii]
AID - 10.1080/13880209.2018.1495748 [doi]
PST - ppublish
SO  - Pharm Biol. 2018 Dec;56(1):422-432. doi: 10.1080/13880209.2018.1495748.