PMID- 30301496
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20181211
IS  - 1165-158X (Electronic)
IS  - 0145-5680 (Linking)
VI  - 64
IP  - 12
DP  - 2018 Sep 30
TI  - Keratinocytes contribute to the recruitment and M1 polarisation of macrophages 
      during C. albicans colonisation.
PG  - 15-21
AB  - Candida albicans (C. albicans) is an opportunistic human fungal pathogen that 
      colonises the skin. Both keratinocytes and macrophages play crucial roles in host 
      defence against C. albicans. However, the interaction of keratinocytes with 
      macrophages during C. albicans colonisation has not been well studied. In this 
      study, macrophages were cultured in conditioned medium from keratinocytes treated 
      with heat-inactivated C. albicans (CM-C. albicans), macrophage migration and 
      polarised activation and were then assessed by a Transwell assay, flow cytometry, 
      quantitative real-time PCR (qPCR), Western blot and an enzyme-linked 
      immunosorbent assay (ELISA). The results showed that CM-C. albicans-stimulated 
      macrophages display significantly increased migration and phagocytosis, and they 
      display an upregulation of proinflammatory cytokines (tumour necrosis factor 
      alpha (TNF-a), interleukin (IL)-12 and nitric oxide (NO)). Markers characteristic 
      of M1 macrophages, such as human leukocyte antigen (HLA)-DR, CD86 and inducible 
      nitric oxide synthase (iNOS), are upregulated, whereas markers of 
      M2 macrophages, such as mannose receptor (MR) and Arginase 1 (Arg1), are not 
      affected. Additionally, the levels of TNF-a, IL-12 and monocyte chemotactic 
      protein 1 (MCP-1) in CM-C. albicans are markedly upregulated, whereas the levels 
      of IL-4 and IL-10 are not affected. And the CM-C. albicans-induced M1 macrophage 
      polarisation, proinflammatory cytokine production and phagocytosis could be 
      blocked by an anti-TNF-a neutralising antibody. This study showed that 
      keratinocytes may promote macrophage recruitment and M1 polarisation during C. 
      albicans colonisation at least in part by secreting TNF-a.
FAU - Guan, Xiu-Hao
AU  - Guan XH
AD  - Department of Dermatology, The First Hospital of China Medical University, 
      No.155, Nan-Jing Street, Shenyang 110001, Liaoning Province, China.
FAU - Hong, Yu-Xiao
AU  - Hong YX
AD  - Department of Dermatology, The First Hospital of China Medical University, 
      No.155, Nan-Jing Street, Shenyang 110001, Liaoning Province, China.
FAU - Qi, Rui-Qun
AU  - Qi RQ
AD  - Department of Dermatology, The First Hospital of China Medical University, 
      No.155, Nan-Jing Street, Shenyang 110001, Liaoning Province, China.
FAU - Gao, Xing-Hua
AU  - Gao XH
AD  - Department of Dermatology, The First Hospital of China Medical University, 
      No.155, Nan-Jing Street, Shenyang 110001, Liaoning Province, China.
LA  - eng
PT  - Journal Article
DEP - 20180930
PL  - France
TA  - Cell Mol Biol (Noisy-le-grand)
JT  - Cellular and molecular biology (Noisy-le-Grand, France)
JID - 9216789
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Blotting, Western
MH  - Candida albicans/*immunology
MH  - Cell Line, Tumor
MH  - Cell Movement/physiology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Keratinocytes/*cytology/*physiology
MH  - Macrophages/*immunology
MH  - Phagocytosis/physiology
MH  - Real-Time Polymerase Chain Reaction
MH  - Tumor Necrosis Factor-alpha/metabolism
OTO - NOTNLM
OT  - C. albicans
OT  - Keratinocyte
OT  - M1 polarisation
OT  - Macrophage
OT  - TNF-a.
EDAT- 2018/10/12 06:00
MHDA- 2018/12/12 06:00
CRDT- 2018/10/11 06:00
PHST- 2018/08/02 00:00 [received]
PHST- 2018/09/12 00:00 [accepted]
PHST- 2018/09/02 00:00 [revised]
PHST- 2018/10/11 06:00 [entrez]
PHST- 2018/10/12 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
PST - epublish
SO  - Cell Mol Biol (Noisy-le-grand). 2018 Sep 30;64(12):15-21.