PMID- 30302013
OWN - NLM
STAT- MEDLINE
DCOM- 20191024
LR  - 20191210
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 8
IP  - 1
DP  - 2018 Oct 9
TI  - Porcine Reproductive and Respiratory Syndrome Virus strains with Higher Virulence 
      Cause Marked Protein Profile Changes in MARC-145 Cells.
PG  - 15000
LID - 10.1038/s41598-018-32984-0 [doi]
LID - 15000
AB  - Porcine reproductive and respiratory syndrome is an infectious disease that 
      causes serious economic losses to the swine industry worldwide. To better 
      understand the pathogenesis of the porcine reproductive and respiratory syndrome 
      virus (PRRSV), three PRRSV strains with different molecular markers and virulence 
      were used to infect MARC-145 cells. A total of 1804 proteins were identified, and 
      233 altered proteins and 72 signaling pathways involved in the proteomic 
      profiling of virus-infected MARC-145 cells increased with the virulence of the 
      PRRSV strain. The three types of viral strains shared a common pathway-the 
      electron transport reaction in mitochondria-in the infected-MARC-145 cells. 
      Moreover, the antisense pathway was the most variable of all significant 
      signaling pathways for the highly virulent SX-1 strain, indicating that this 
      unique pathway may be connected to the high virulence of the SX-1 strain. Our 
      study is the first attempt to provide a proteome profile of MARC-145 cells 
      infected with PRRSV strains with different virulence, and these findings will 
      facilitate a deep understanding of the interactions between this virus and its 
      host.
FAU - Chen, Zhi
AU  - Chen Z
AD  - Shandong Key Lab of Animal Disease Control and Breeding, Shandong Academy of 
      Agricultural Sciences, Jinan, 250100, China.
AD  - College of Life Sciences, Shandong Normal University, Jinan, 250014, China.
FAU - Liu, Shaoning
AU  - Liu S
AD  - Shandong Institute of Veterinary Drug Quality Inspection, Jinan, 250022, China.
FAU - Zhang, Shujin
AU  - Zhang S
AD  - College of Agronomy, Liaocheng University, Liaocheng, 252000, China.
FAU - Zhang, Yuyu
AU  - Zhang Y
AD  - Shandong Key Lab of Animal Disease Control and Breeding, Shandong Academy of 
      Agricultural Sciences, Jinan, 250100, China.
AD  - College of Life Sciences, Shandong Normal University, Jinan, 250014, China.
FAU - Yu, Jiang
AU  - Yu J
AD  - Shandong Key Lab of Animal Disease Control and Breeding, Shandong Academy of 
      Agricultural Sciences, Jinan, 250100, China.
AD  - College of Life Sciences, Shandong Normal University, Jinan, 250014, China.
FAU - Sun, Wenbo
AU  - Sun W
AD  - Shandong Key Lab of Animal Disease Control and Breeding, Shandong Academy of 
      Agricultural Sciences, Jinan, 250100, China.
FAU - Chen, Lei
AU  - Chen L
AD  - Shandong Key Lab of Animal Disease Control and Breeding, Shandong Academy of 
      Agricultural Sciences, Jinan, 250100, China.
AD  - College of Life Sciences, Shandong Normal University, Jinan, 250014, China.
FAU - Du, Yijun
AU  - Du Y
AUID- ORCID: 0000-0001-9215-4637
AD  - Shandong Key Lab of Animal Disease Control and Breeding, Shandong Academy of 
      Agricultural Sciences, Jinan, 250100, China.
FAU - Wang, Jinbao
AU  - Wang J
AD  - Shandong Key Lab of Animal Disease Control and Breeding, Shandong Academy of 
      Agricultural Sciences, Jinan, 250100, China.
FAU - Li, Yubao
AU  - Li Y
AD  - College of Agronomy, Liaocheng University, Liaocheng, 252000, China.
FAU - Wu, Jiaqiang
AU  - Wu J
AD  - Shandong Key Lab of Animal Disease Control and Breeding, Shandong Academy of 
      Agricultural Sciences, Jinan, 250100, China. wujiaqiang2000@sina.com.
AD  - College of Life Sciences, Shandong Normal University, Jinan, 250014, China. 
      wujiaqiang2000@sina.com.
LA  - eng
GR  - 31602076/National Natural Science Foundation of China (National Science 
      Foundation of China)/International
GR  - 31572550/National Natural Science Foundation of China (National Science 
      Foundation of China)/International
GR  - 31340047/National Natural Science Foundation of China (National Science 
      Foundation of China)/International
GR  - BS2014NY009/B.S. Foundation of Shandong Province (Doctoral Foundation of Shandong 
      Province)/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181009
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Antibodies, Viral)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/metabolism
MH  - *Base Sequence
MH  - Chlorocebus aethiops
MH  - *Macrophages, Alveolar/metabolism/pathology/virology
MH  - *Porcine Reproductive and Respiratory Syndrome/genetics/metabolism/pathology
MH  - *Porcine respiratory and reproductive syndrome 
      virus/genetics/metabolism/pathogenicity
MH  - *Sequence Deletion
MH  - Swine
MH  - Vero Cells
MH  - *Viral Proteins/biosynthesis/genetics
PMC - PMC6177479
COIS- The authors declare no competing interests.
EDAT- 2018/10/12 06:00
MHDA- 2019/10/28 06:00
PMCR- 2018/10/09
CRDT- 2018/10/11 06:00
PHST- 2018/05/31 00:00 [received]
PHST- 2018/09/17 00:00 [accepted]
PHST- 2018/10/11 06:00 [entrez]
PHST- 2018/10/12 06:00 [pubmed]
PHST- 2019/10/28 06:00 [medline]
PHST- 2018/10/09 00:00 [pmc-release]
AID - 10.1038/s41598-018-32984-0 [pii]
AID - 32984 [pii]
AID - 10.1038/s41598-018-32984-0 [doi]
PST - epublish
SO  - Sci Rep. 2018 Oct 9;8(1):15000. doi: 10.1038/s41598-018-32984-0.