PMID- 30302801
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230202
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 120
IP  - 2
DP  - 2019 Feb
TI  - MiR-143-3p suppresses cell proliferation, migration, and invasion by targeting 
      Melanoma-Associated Antigen A9 in laryngeal squamous cell carcinoma.
PG  - 1245-1257
LID - 10.1002/jcb.27084 [doi]
AB  - Previously we found that melanoma-associated antigen-A9 (MAGE-A9) was a 
      significantly upregulated biomarker in laryngeal squamous cell carcinoma (LSCC). 
      A high expression of MAGE-A9 indicates an unfavorable survival outcome, and the 
      MAGE-A9 expression level is an independent prognostic factor of LSCC. To explore 
      the mechanism of MAGE-A9 upregulation, several predicted regulatory microRNAs 
      were screened and validated in LSCC cells. In the current study, we found that 
      miR-143-3p (MAGE-A9 related miRNAs) expression levels correlated negatively with 
      the MAGE-A9 protein expression in LSCC tissues. Dual-luciferase reporter assays 
      and Western blot analysis revealed MAGE-A9 to be a direct target of miR-143-3p. 
      Furthermore, a series of in vitro gain- and loss-of-function assays revealed that 
      miR-143-3p inhibited LSCC cell proliferation, migration, and invasion. Also, 
      miR-143-3p suppressed LSCC tumorigenesis in vivo. These effects were clinically 
      relevant, as a lower expression of miR-143-3p occurred in severer clinical stages 
      and represented poor overall survival in patients with LSCC. Taken together, 
      these results suggest that downregulation of miR-143-3p contributes to tumor 
      progression through upregulation of MAGE-A9. The expression level of these two 
      key molecules maintained LSCC progression, thus, highlighting the potential of 
      miR-143-3p as a therapeutic target for human LSCC.
CI  - (c) 2018 Wiley Periodicals, Inc.
FAU - Han, Liang
AU  - Han L
AD  - Department of Head and Neck Surgery, Nantong Tumor Hospital, Affiliated Tumor 
      Hospital of Nantong University, Nantong, Jiangsu, China.
FAU - Tang, Mingming
AU  - Tang M
AD  - Department of Head and Neck Surgery, Nantong Tumor Hospital, Affiliated Tumor 
      Hospital of Nantong University, Nantong, Jiangsu, China.
FAU - Xu, Xinjiang
AU  - Xu X
AD  - Department of Head and Neck Surgery, Nantong Tumor Hospital, Affiliated Tumor 
      Hospital of Nantong University, Nantong, Jiangsu, China.
FAU - Jiang, Bin
AU  - Jiang B
AD  - Department of Head and Neck Surgery, Nantong Tumor Hospital, Affiliated Tumor 
      Hospital of Nantong University, Nantong, Jiangsu, China.
FAU - Wei, Yingze
AU  - Wei Y
AD  - Department of Clinical Pathology, Nantong Tumor Hospital, Affiliated Tumor 
      Hospital of Nantong University, Nantong, Jiangsu, China.
FAU - Qian, Hongyan
AU  - Qian H
AD  - Cancer Research Center, Nantong Tumor Hospi tal, Affiliated Tumor Hospital of 
      Nantong University, Nantong, Jiangsu, China.
FAU - Lu, Xueguan
AU  - Lu X
AUID- ORCID: 0000-0001-9829-3312
AD  - Department of Radiation Oncology, Fudan University Shanghai Cancer Center, 
      Shanghai, China.
LA  - eng
GR  - MS22015108/Nantong Science and Technique Development Fund/
GR  - 17ZR1406100/Natural Science Foundation of Shanghai/
PT  - Journal Article
DEP - 20181009
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
SB  - IM
OTO - NOTNLM
OT  - invasion
OT  - laryngeal squamous cell carcinoma (LSCC)
OT  - melanoma-associated antigens-A9 (MAGE-A9)
OT  - miR-143-3p
OT  - migration
OT  - proliferation
EDAT- 2018/10/12 06:00
MHDA- 2018/10/12 06:01
CRDT- 2018/10/11 06:00
PHST- 2017/11/07 00:00 [received]
PHST- 2018/04/26 00:00 [accepted]
PHST- 2018/10/12 06:00 [pubmed]
PHST- 2018/10/12 06:01 [medline]
PHST- 2018/10/11 06:00 [entrez]
AID - 10.1002/jcb.27084 [doi]
PST - ppublish
SO  - J Cell Biochem. 2019 Feb;120(2):1245-1257. doi: 10.1002/jcb.27084. Epub 2018 Oct 
      9.