PMID- 30303616
OWN - NLM
STAT- MEDLINE
DCOM- 20190118
LR  - 20190118
IS  - 1476-4431 (Electronic)
IS  - 1476-4431 (Linking)
VI  - 28
IP  - 6
DP  - 2018 Nov
TI  - A pilot study documenting increased thrombin generation following abrupt 
      withdrawal of heparin therapy in healthy dogs.
PG  - 518-526
LID - 10.1111/vec.12778 [doi]
AB  - OBJECTIVE: To document if a transient hypercoagulable state occurs in healthy 
      dogs following abrupt cessation of unfractionated heparin (UFH) therapy. DESIGN: 
      Prospective experimental pilot study. SETTING: University research facility. 
      ANIMALS: Seven adult random-source male dogs. INTERVENTION: Thromboelastography 
      (TEG) and thrombin-antithrombin (TAT) complex formation were used to assess 
      coagulation status in healthy dogs. Seven adult research dogs received 200-300 
      IU/kg subcutaneous UFH every 8 hours for 4 days. A final IV bolus of 100 IU/kg 
      was given on day 4 and the peak measured heparin concentration 1 hour later is 
      defined as the start of heparin withdrawal (time 0). Citrated whole blood samples 
      were collected at baseline (prior to heparin administration) and 3, 6, 12, 30, 
      and 48 hours after UFH withdrawal. At all time points, a kaolin-activated TEG was 
      performed and citrated plasma for measurement of TAT concentration was collected 
      for batch analysis. Fibrinogen concentration, PCV, total plasma proteins, and 
      platelet count were measured at baseline and 48 hours after heparin withdrawal. 
      MEASUREMENTS AND MAIN RESULTS: Compared to baseline, TAT was increased 12 hours 
      after heparin withdrawal and returned to baseline by 30 hours. TEG clot formation 
      time (K) was decreased 30 and 48 hours after heparin withdrawal. CONCLUSION: TAT 
      results suggest that a transient increase in thrombin generation developed 12 
      hours after withdrawal of UFH therapy. Though clot kinetics were rapid compared 
      to baseline beginning 30 hours after heparin withdrawal, a return to baseline was 
      not documented. Future studies are warranted to determine the clinical relevance 
      of these results and to evaluate the effect of UFH withdrawal in critically ill 
      animals.
CI  - (c) Veterinary Emergency and Critical Care Society 2018.
FAU - Mays, Erin M
AU  - Mays EM
AUID- ORCID: 0000-0002-7088-6375
AD  - Departments of Clinical Sciences, North Carolina State University College of 
      Veterinary Medicine, Raleigh, NC 27695 (Mays, Daorman, Hanel), and.
FAU - Dorman, David C
AU  - Dorman DC
AD  - Departments of Clinical Sciences, North Carolina State University College of 
      Veterinary Medicine, Raleigh, NC 27695 (Mays, Daorman, Hanel), and.
FAU - McKendry, Colleen
AU  - McKendry C
AD  - Department of Statistics, NCSU Bioinformatics Research Center, NCSU College of 
      Physical and Mathematical Sciences, Raleigh, NC, 27607 (McKendry).
FAU - Hanel, Rita M
AU  - Hanel RM
AUID- ORCID: 0000-0002-2814-1003
AD  - Departments of Clinical Sciences, North Carolina State University College of 
      Veterinary Medicine, Raleigh, NC 27695 (Mays, Daorman, Hanel), and.
LA  - eng
PT  - Clinical Trial, Veterinary
PT  - Journal Article
DEP - 20181010
PL  - United States
TA  - J Vet Emerg Crit Care (San Antonio)
JT  - Journal of veterinary emergency and critical care (San Antonio, Tex. : 2001)
JID - 101152804
RN  - 0 (Anticoagulants)
RN  - 9005-49-6 (Heparin)
RN  - EC 3.4.21.5 (Thrombin)
SB  - IM
MH  - Animals
MH  - Anticoagulants/administration & dosage/*pharmacology
MH  - Dogs
MH  - Heparin/administration & dosage/*pharmacology
MH  - Infusions, Intravenous/veterinary
MH  - Male
MH  - Pilot Projects
MH  - Prospective Studies
MH  - Thrombelastography/veterinary
MH  - Thrombin/biosynthesis/*drug effects
OTO - NOTNLM
OT  - hypercoagulable
OT  - rebound
OT  - thromboelastography
EDAT- 2018/10/12 06:00
MHDA- 2019/01/19 06:00
CRDT- 2018/10/11 06:00
PHST- 2016/11/22 00:00 [received]
PHST- 2017/04/12 00:00 [revised]
PHST- 2017/05/24 00:00 [accepted]
PHST- 2018/10/12 06:00 [pubmed]
PHST- 2019/01/19 06:00 [medline]
PHST- 2018/10/11 06:00 [entrez]
AID - 10.1111/vec.12778 [doi]
PST - ppublish
SO  - J Vet Emerg Crit Care (San Antonio). 2018 Nov;28(6):518-526. doi: 
      10.1111/vec.12778. Epub 2018 Oct 10.