PMID- 30304557
OWN - NLM
STAT- MEDLINE
DCOM- 20200619
LR  - 20200619
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 120
IP  - 3
DP  - 2019 Mar
TI  - Long noncoding RNA PVT1 promotes laryngeal squamous cell carcinoma development by 
      acting as a molecular sponge to regulate miR-519d-3p.
PG  - 3911-3921
LID - 10.1002/jcb.27673 [doi]
AB  - OBJECTIVE: This study was designed to investigate the effects and mechanism of 
      long noncoding RNA (lncRNA) PVT1 on cell migration, proliferation, and apoptosis 
      of laryngeal squamous cell carcinoma (LSCC). METHODS: We screened lncRNAs 
      expression profiles in four pair LSCC and matched noncancerous tissues by 
      microarray assay. The messenger RNA levels of PVT1 in tissues and cells were 
      evaluated by quantitative real-time polymerase chain reaction analysis. StarBase 
      website was used to predict the target miRNAs for PVT1. And the interaction 
      between PVT1 and target miRNA-519d-3p in LSCC cells was analyzed using 
      dual-luciferase reporter assay. MTT assay was used to investigate the cell 
      viability. Cell counting assay was used to explore the cell proliferation. 
      Annexin-V propidium iodide flow cytometry was used to examine the cell apoptosis, 
      and transwell assay was used to investigate the effects of lncRNA PVT1 on cell 
      migration. RESULTS: PVT1 was significantly overexpressed in human LSCC tissues 
      and several LSCC cell lines. Upregulation of lncRNA PVT1 markedly facilitated 
      proliferation suppressed apoptosis and promoted cell migration in LSCC cells. We 
      further demonstrated that silencing PVT1 strikingly suppressed proliferation, 
      promoted apoptosis, and reduced migration in LSCC cells. Further bioinformatic 
      analysis and dual-luciferase reporter assay revealed that PVT1 could function as 
      an oncogenic transcript partly through sponging miR-519d-3p. Besides, mechanistic 
      investigations indicated that PVT1 could promote cell and migration through 
      interacting with miR-519d-3p. CONCLUSION: LncRNA PVT1 is consistently 
      overexpressed in human LSCC, and overexpression of lncRNA PVT1 contributes to the 
      proliferation and migration of LSCC through inhibiting miR-519d-3p expression.
CI  - (c) 2018 Wiley Periodicals, Inc.
FAU - Zheng, Xiling
AU  - Zheng X
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The Second Affiliated 
      Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
FAU - Zhao, Kang
AU  - Zhao K
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Xi'an Jiaotong 
      University, Xi'an, Shaanxi, China.
FAU - Liu, Tao
AU  - Liu T
AD  - Department of General Surgery, Yan'an University Affiliated Hospital, Yan'an, 
      Shaanxi, China.
FAU - Liu, Lei
AU  - Liu L
AD  - Department of General Surgery, Yan'an University Affiliated Hospital, Yan'an, 
      Shaanxi, China.
FAU - Zhou, Changming
AU  - Zhou C
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Xi'an Jiaotong 
      University, Xi'an, Shaanxi, China.
FAU - Xu, Min
AU  - Xu M
AUID- ORCID: 0000-0002-2923-5837
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The Second Affiliated 
      Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181010
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Antagomirs)
RN  - 0 (MIRN519 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Oligoribonucleotides)
RN  - 0 (PVT1 long-non-coding RNA, human)
RN  - 0 (RNA, Long Noncoding)
SB  - IM
MH  - Antagomirs/genetics/metabolism
MH  - Apoptosis/genetics
MH  - Base Sequence
MH  - Carcinoma, Squamous Cell/*genetics/metabolism/pathology/surgery
MH  - Cell Line
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Cell Proliferation
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/metabolism/pathology/surgery
MH  - MicroRNAs/*genetics/metabolism
MH  - Neoplasm Invasiveness
MH  - Oligoribonucleotides/genetics/metabolism
MH  - RNA, Long Noncoding/agonists/antagonists & inhibitors/*genetics/metabolism
MH  - Signal Transduction
OTO - NOTNLM
OT  - laryngeal squamous cell carcinoma
OT  - long noncoding RNA PVT1
OT  - miR-519d-3p
EDAT- 2018/10/12 06:00
MHDA- 2020/06/20 06:00
CRDT- 2018/10/11 06:00
PHST- 2018/05/04 00:00 [received]
PHST- 2018/08/21 00:00 [accepted]
PHST- 2018/10/12 06:00 [pubmed]
PHST- 2020/06/20 06:00 [medline]
PHST- 2018/10/11 06:00 [entrez]
AID - 10.1002/jcb.27673 [doi]
PST - ppublish
SO  - J Cell Biochem. 2019 Mar;120(3):3911-3921. doi: 10.1002/jcb.27673. Epub 2018 Oct 
      10.