PMID- 3030634
OWN - NLM
STAT- MEDLINE
DCOM- 19870427
LR  - 20190828
IS  - 0271-3683 (Print)
IS  - 0271-3683 (Linking)
VI  - 6
IP  - 1
DP  - 1987 Jan
TI  - Identifying HSV infected neurons after ocular inoculation.
PG  - 119-26
AB  - ICR mice were inoculated intracamerally with McKrae strain herpes simplex virus 
      (HSV) followed by intraperitoneal injection with 3H-thymidine. Infected mice were 
      sacrificed after 3 or 4 days and the eyes, trigeminal ganglia (TG) and superior 
      cervical ganglia (SCG) were embedded in glycol methacrylate, sectioned, and 
      dipped for autoradiography. Light microscopy revealed silver grain labeling over 
      neurons in the ipsilateral retina, TG and SCG of infected animals. No labeling of 
      neurons was noted in the contralateral TG or SCG. Since the DNA of mature neurons 
      does not replicate, we interpret these labeled neurons to represent cells with 
      active replication of HSV. This technique allows the study of HSV infection of 
      the nervous system with excellent tissue preservation. Furthermore, it may be 
      used to distinguish those neurons with intrinsic viral synthesis from those 
      harboring virus synthesized at a distant site with subsequent intracellular 
      spread.
FAU - Margolis, T
AU  - Margolis T
FAU - Togni, B
AU  - Togni B
FAU - LaVail, J
AU  - LaVail J
FAU - Dawson, C R
AU  - Dawson CR
LA  - eng
GR  - EY02162/EY/NEI NIH HHS/United States
GR  - EY03917/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Curr Eye Res
JT  - Current eye research
JID - 8104312
SB  - IM
MH  - Animals
MH  - Autoradiography
MH  - Eye/*innervation
MH  - Histocytochemistry
MH  - Keratitis, Dendritic/*microbiology
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Microscopy, Electron
MH  - Neurons/*microbiology/ultrastructure
MH  - Simplexvirus/*isolation & purification
EDAT- 1987/01/01 00:00
MHDA- 1987/01/01 00:01
CRDT- 1987/01/01 00:00
PHST- 1987/01/01 00:00 [pubmed]
PHST- 1987/01/01 00:01 [medline]
PHST- 1987/01/01 00:00 [entrez]
AID - 10.3109/02713688709020078 [doi]
PST - ppublish
SO  - Curr Eye Res. 1987 Jan;6(1):119-26. doi: 10.3109/02713688709020078.