PMID- 30306456
OWN - NLM
STAT- MEDLINE
DCOM- 20190419
LR  - 20231006
IS  - 1573-4919 (Electronic)
IS  - 0300-8177 (Print)
IS  - 0300-8177 (Linking)
VI  - 454
IP  - 1-2
DP  - 2019 Apr
TI  - Genes interconnecting AMPK and TREM-1 and associated microRNAs in rotator cuff 
      tendon injury.
PG  - 97-109
LID - 10.1007/s11010-018-3456-z [doi]
AB  - Fatty infiltration and inflammation delay the healing responses and raise major 
      concerns in the therapeutic management of rotator cuff tendon injuries (RCTI). 
      Our evaluations showed the upregulation of 'metabolic check point' AMPK and 
      inflammatory molecule, TREM-1 from shoulder biceps tendons collected from RCTI 
      subjects. However, the epigenetic regulation of these biomolecules by miRNAs is 
      largely unknown and it is likely that a deeper understanding of the mechanism of 
      action can have therapeutic potential for RCTI. Based on this background, we have 
      evaluated the miRNAs from RCTI patients with fatty infiltration and inflammation 
      (FI group) and compared with RCTI patients without fatty infiltration and 
      inflammation (No-FI group). NetworkAnalyst was employed to evaluate the genes 
      interconnecting AMPK and TREM-1 pathway, using PRKAA1 (AMPK), TREM-1, HIF1alpha, 
      HMGB1, and AGER as input genes. The most relevant miRNAs were screened by 
      considering the fold change below - 7.5 and the number of target genes 10 and 
      more which showed 13 miRNAs and 216 target genes. The exact role of these miRNAs 
      in the fatty infiltration and inflammation associated with RCTI is still unknown 
      and the understanding of biological activity of these miRNAs can pave ways to 
      develop miRNA-based therapeutics in the management of RCTI.
FAU - Thankam, Finosh G
AU  - Thankam FG
AD  - Departments of Clinical & Translational Science and Orthopedic Surgery, Creighton 
      University School of Medicine, Omaha, NE, 68178, USA.
FAU - Boosani, Chandra S
AU  - Boosani CS
AD  - Departments of Clinical & Translational Science and Orthopedic Surgery, Creighton 
      University School of Medicine, Omaha, NE, 68178, USA.
FAU - Dilisio, Matthew F
AU  - Dilisio MF
AD  - Departments of Clinical & Translational Science and Orthopedic Surgery, Creighton 
      University School of Medicine, Omaha, NE, 68178, USA.
FAU - Gross, R Michael
AU  - Gross RM
AD  - Departments of Clinical & Translational Science and Orthopedic Surgery, Creighton 
      University School of Medicine, Omaha, NE, 68178, USA.
FAU - Agrawal, Devendra K
AU  - Agrawal DK
AUID- ORCID: 0000-0001-5445-0013
AD  - Departments of Clinical & Translational Science and Orthopedic Surgery, Creighton 
      University School of Medicine, Omaha, NE, 68178, USA. dkagr@creighton.edu.
AD  - Department of Clinical & Translational Science, The Peekie Nash Carpenter Endowed 
      Chair in Medicine, CRISS II Room 510, 2500 California Plaza, Omaha, NE, 68178, 
      USA. dkagr@creighton.edu.
LA  - eng
GR  - R01HL116042/National Institutes of Health/
GR  - R01 HL116042/HL/NHLBI NIH HHS/United States
GR  - R01HL120659/National Institutes of Health/
GR  - R01HL144125/National Heart, Lung, and Blood Institute/
GR  - R01 HL144125/HL/NHLBI NIH HHS/United States
GR  - R01 HL120659/HL/NHLBI NIH HHS/United States
PT  - Journal Article
DEP - 20181010
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (MicroRNAs)
RN  - 0 (TREM1 protein, human)
RN  - 0 (Triggering Receptor Expressed on Myeloid Cells-1)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
SB  - IM
MH  - AMP-Activated Protein Kinases/metabolism
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation
MH  - Humans
MH  - *Inflammation
MH  - MicroRNAs/genetics/*metabolism
MH  - Rotator Cuff/*metabolism/pathology
MH  - *Signal Transduction
MH  - Tendon Injuries/*metabolism/pathology
MH  - Triggering Receptor Expressed on Myeloid Cells-1/metabolism
PMC - PMC6438203
MID - NIHMS1524745
OTO - NOTNLM
OT  - AMPK signaling
OT  - Fatty infiltration
OT  - HMGB1
OT  - Inflammation
OT  - Rotator cuff tendon injury
OT  - TREM-1
OT  - miRNA
COIS- Disclosure statement The authors have no conflict of interest to disclose.
EDAT- 2018/10/12 06:00
MHDA- 2019/04/20 06:00
PMCR- 2020/04/01
CRDT- 2018/10/12 06:00
PHST- 2018/05/15 00:00 [received]
PHST- 2018/10/05 00:00 [accepted]
PHST- 2018/10/12 06:00 [pubmed]
PHST- 2019/04/20 06:00 [medline]
PHST- 2018/10/12 06:00 [entrez]
PHST- 2020/04/01 00:00 [pmc-release]
AID - 10.1007/s11010-018-3456-z [pii]
AID - 10.1007/s11010-018-3456-z [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2019 Apr;454(1-2):97-109. doi: 10.1007/s11010-018-3456-z. Epub 
      2018 Oct 10.