PMID- 30308007
OWN - NLM
STAT- MEDLINE
DCOM- 20190326
LR  - 20240314
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 13
IP  - 10
DP  - 2018
TI  - Thy1 transgenic mice expressing the red fluorescent calcium indicator jRGECO1a 
      for neuronal population imaging in vivo.
PG  - e0205444
LID - 10.1371/journal.pone.0205444 [doi]
LID - e0205444
AB  - Calcium imaging is commonly used to measure the neural activity of large groups 
      of neurons in mice. Genetically encoded calcium indicators (GECIs) can be 
      delivered for this purpose using non-invasive genetic methods. Compared to viral 
      gene transfer, transgenic targeting of GECIs provides stable long-term expression 
      and obviates the need for invasive viral injections. Transgenic mice expressing 
      the green GECI GCaMP6 are already widely used. Here we present the generation and 
      characterization of transgenic mice expressing the sensitive red GECI jRGECO1a, 
      driven by the Thy1 promoter. Four transgenic lines with different expression 
      patterns showed sufficiently high expression for cellular in vivo imaging. We 
      used two-photon microscopy to characterize visual responses of individual neurons 
      in the visual cortex in vivo. The signal-to-noise ratio in transgenic mice was 
      comparable to, or better than, mice transduced with adeno-associated virus. In 
      addition, we show that Thy1-jRGECO1a transgenic mice are useful for transcranial 
      population imaging and functional mapping using widefield fluorescence 
      microscopy. We also demonstrate imaging of visual responses in retinal ganglion 
      cells in vitro. Thy1-jRGECO1a transgenic mice are therefore a useful addition to 
      the toolbox for imaging activity in intact neural networks.
FAU - Dana, Hod
AU  - Dana H
AUID- ORCID: 0000-0002-8093-2363
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, United 
      States of America.
FAU - Novak, Ondrej
AU  - Novak O
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, United 
      States of America.
AD  - Department of Auditory Neuroscience, Institute of Experimental Medicine, Academy 
      of Sciences of the Czech Republic, Prague, Czech Republic.
FAU - Guardado-Montesino, Michael
AU  - Guardado-Montesino M
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, United 
      States of America.
FAU - Fransen, James W
AU  - Fransen JW
AD  - Department of Anatomical Sciences and Neurobiology, University of Louisville 
      School of Medicine, Louisville, KY, United States of America.
FAU - Hu, Amy
AU  - Hu A
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, United 
      States of America.
FAU - Borghuis, Bart G
AU  - Borghuis BG
AD  - Department of Anatomical Sciences and Neurobiology, University of Louisville 
      School of Medicine, Louisville, KY, United States of America.
FAU - Guo, Caiying
AU  - Guo C
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, United 
      States of America.
FAU - Kim, Douglas S
AU  - Kim DS
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, United 
      States of America.
FAU - Svoboda, Karel
AU  - Svoboda K
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, United 
      States of America.
LA  - eng
SI  - figshare/10.6084/m9.figshare.7117544.v1
GR  - R01 EY028188/EY/NEI NIH HHS/United States
GR  - HHMI/Howard Hughes Medical Institute/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20181011
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Luminescent Proteins)
RN  - 0 (Thy-1 Antigens)
SB  - IM
MH  - Animals
MH  - Luminescent Proteins/genetics/*metabolism
MH  - Mice
MH  - Mice, Transgenic
MH  - Microscopy, Fluorescence
MH  - Neurons/*metabolism
MH  - Promoter Regions, Genetic
MH  - Signal-To-Noise Ratio
MH  - Thy-1 Antigens/*genetics
MH  - Visual Cortex/*diagnostic imaging/metabolism
PMC - PMC6181368
COIS- The authors have declared that no competing interests exist.
EDAT- 2018/10/12 06:00
MHDA- 2019/03/27 06:00
PMCR- 2018/10/11
CRDT- 2018/10/12 06:00
PHST- 2018/06/18 00:00 [received]
PHST- 2018/09/25 00:00 [accepted]
PHST- 2018/10/12 06:00 [entrez]
PHST- 2018/10/12 06:00 [pubmed]
PHST- 2019/03/27 06:00 [medline]
PHST- 2018/10/11 00:00 [pmc-release]
AID - PONE-D-18-16867 [pii]
AID - 10.1371/journal.pone.0205444 [doi]
PST - epublish
SO  - PLoS One. 2018 Oct 11;13(10):e0205444. doi: 10.1371/journal.pone.0205444. 
      eCollection 2018.