PMID- 30308117
OWN - NLM
STAT- MEDLINE
DCOM- 20191213
LR  - 20200725
IS  - 1521-6551 (Electronic)
IS  - 1521-6543 (Print)
IS  - 1521-6543 (Linking)
VI  - 71
IP  - 1
DP  - 2019 Jan
TI  - MicroRNA-107 regulates anesthesia-induced neural injury in embryonic stem cell 
      derived neurons.
PG  - 20-27
LID - 10.1002/iub.1911 [doi]
AB  - Ketamine, though widely used in pediatric anesthesia, may induce cortical 
      neurotoxicity in young patients. This study focused on an in vitro model of rat 
      brain embryonic stem cell (ESC)-derived neurons to investigate the effects of 
      microRNA-107 (miR-107) on ketamine-induced neural injury. Rat brain ESCs were 
      proliferated in vitro and differentiated toward neuronal fate. Ketamine induced 
      neural injury in ESC-derived neurons was inspected by TUNEL and neurite growth 
      assays. Ketamine-induce aberrant miR-107 expression was examined by qRT-PCR. 
      MiR-107 was downregulated in ESCs through lentiviral transduction. Its effect on 
      ketamine-induced neural injury in ESC-derived neurons was then examined. 
      Potential downstream target of miR-107, brain derived neurotrophin factor (BDNF), 
      was inspected by dual-luciferase reporter assay and qRT-PCR. BDNF was knocked 
      down, through siRNA transfection, in NSCs to investigate its functional 
      involvement in miR-107 mediated neural protection in ketamine-injured NSC-derived 
      neurons. Ketamine induced apoptosis, neurite degeneration, and upregulated 
      miR-107 in NSC-derived neurons. Lentivirus-mediated miR-107 downregulation 
      attenuated ketamine-induced neural injury. BDNF was proven to be directly and 
      inversely regulated by miR-107 in NSC-derived neurons. SiRNA-mediated BDNF 
      inhibition reversed the protective effect of miR-107 downregulation on ketamine 
      injury in NSC-derived neurons. MiR-107 / BDNF was demonstrated to be an important 
      epigenetic signaling pathway in regulating ketamine-induced neural injury in 
      cortical neurons. (c) 2018 The Authors. IUBMB Life published by Wiley 
      Periodicals,Inc. on behalf of International Union of Biochemistry and Molecular 
      Biology., 71(1):20-27, 2019.
CI  - (c) 2018 The Authors. IUBMB Life published by Wiley Periodicals, Inc. on behalf of 
      International Union of Biochemistry and Molecular Biology.
FAU - Jiang, Jun Dan
AU  - Jiang JD
AD  - Department of Anesthesiology, Fujian Provincial Hospital, Provincial Clinical 
      Medical College, Fujian Medical University, Fujian, China.
FAU - Zheng, Xiao Chun
AU  - Zheng XC
AUID- ORCID: 0000-0002-6213-0789
AD  - Department of Anesthesiology, Fujian Provincial Hospital, Provincial Clinical 
      Medical College, Fujian Medical University, Fujian, China.
AD  - Department of Anesthesiology, Fujian Provincial Emergency Center, Provincial 
      Clinical Medical College, Fujian Medical University, Fujian, China.
FAU - Huang, Feng Yi
AU  - Huang FY
AD  - Department of Anesthesiology, Fujian Provincial Hospital, Provincial Clinical 
      Medical College, Fujian Medical University, Fujian, China.
FAU - Gao, Fei
AU  - Gao F
AD  - Department of Anesthesiology, Fujian Provincial Hospital, Provincial Clinical 
      Medical College, Fujian Medical University, Fujian, China.
FAU - You, Mei Zhen
AU  - You MZ
AD  - Department of Anesthesiology, Fujian Provincial Hospital, Provincial Clinical 
      Medical College, Fujian Medical University, Fujian, China.
FAU - Zheng, Ting
AU  - Zheng T
AD  - Department of Anesthesiology, Fujian Provincial Hospital, Provincial Clinical 
      Medical College, Fujian Medical University, Fujian, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181011
PL  - England
TA  - IUBMB Life
JT  - IUBMB life
JID - 100888706
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (MIRN107 microRNA, rat)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Small Interfering)
RN  - 690G0D6V8H (Ketamine)
SB  - IM
MH  - Anesthesia/adverse effects
MH  - Animals
MH  - Apoptosis/genetics
MH  - Brain Injuries/chemically induced
MH  - Brain-Derived Neurotrophic Factor/*genetics
MH  - Cell Differentiation/genetics
MH  - Embryonic Stem Cells/drug effects/metabolism
MH  - Gene Expression Regulation, Developmental/drug effects
MH  - Humans
MH  - Ketamine/adverse effects
MH  - MicroRNAs/antagonists & inhibitors/*genetics
MH  - Neurites/drug effects/pathology
MH  - Neurogenesis/*genetics
MH  - Neurons/*metabolism/pathology
MH  - Neurotoxicity Syndromes/genetics/pathology
MH  - RNA, Small Interfering/genetics
MH  - Rats
MH  - Signal Transduction/drug effects
PMC - PMC7379593
OTO - NOTNLM
OT  - BDNF
OT  - embryonic stem cell
OT  - ketamine
OT  - miR-107
OT  - microRNA
OT  - neural injury
COIS- None.
EDAT- 2018/10/12 06:00
MHDA- 2019/12/18 06:00
PMCR- 2020/07/24
CRDT- 2018/10/12 06:00
PHST- 2018/04/07 00:00 [received]
PHST- 2018/05/07 00:00 [accepted]
PHST- 2018/10/12 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
PHST- 2018/10/12 06:00 [entrez]
PHST- 2020/07/24 00:00 [pmc-release]
AID - IUB1911 [pii]
AID - 10.1002/iub.1911 [doi]
PST - ppublish
SO  - IUBMB Life. 2019 Jan;71(1):20-27. doi: 10.1002/iub.1911. Epub 2018 Oct 11.