PMID- 30310066 OWN - NLM STAT- MEDLINE DCOM- 20190107 LR - 20220129 IS - 2041-1723 (Electronic) IS - 2041-1723 (Linking) VI - 9 IP - 1 DP - 2018 Oct 11 TI - Mechanochemical feedback control of dynamin independent endocytosis modulates membrane tension in adherent cells. PG - 4217 LID - 10.1038/s41467-018-06738-5 [doi] LID - 4217 AB - Plasma membrane tension regulates many key cellular processes. It is modulated by, and can modulate, membrane trafficking. However, the cellular pathway(s) involved in this interplay is poorly understood. Here we find that, among a number of endocytic processes operating simultaneously at the cell surface, a dynamin independent pathway, the CLIC/GEEC (CG) pathway, is rapidly and specifically upregulated upon a sudden reduction of tension. Moreover, inhibition (activation) of the CG pathway results in lower (higher) membrane tension. However, alteration in membrane tension does not directly modulate CG endocytosis. This requires vinculin, a mechano-transducer recruited to focal adhesion in adherent cells. Vinculin acts by controlling the levels of a key regulator of the CG pathway, GBF1, at the plasma membrane. Thus, the CG pathway directly regulates membrane tension and is in turn controlled via a mechano-chemical feedback inhibition, potentially leading to homeostatic regulation of membrane tension in adherent cells. FAU - Thottacherry, Joseph Jose AU - Thottacherry JJ AD - National Centre for Biological Sciences (NCBS), Tata Institute of Fundamental Research (TIFR), Bellary Road, Bengaluru, 560065, India. FAU - Kosmalska, Anita Joanna AU - Kosmalska AJ AD - Institute for Bioengineering of Catalonia (IBEC), Barcelona, 08028, Spain. AD - University of Barcelona, Barcelona, 08036, Spain. FAU - Kumar, Amit AU - Kumar A AD - Raman Research Institute, C. V. Raman Avenue, Bengaluru, 560080, India. FAU - Vishen, Amit Singh AU - Vishen AS AUID- ORCID: 0000-0001-8938-0852 AD - National Centre for Biological Sciences (NCBS), Tata Institute of Fundamental Research (TIFR), Bellary Road, Bengaluru, 560065, India. AD - Simons Centre for the Study of Living Machines, National Centre for Biological Sciences (NCBS), Bengaluru, 560065, India. FAU - Elosegui-Artola, Alberto AU - Elosegui-Artola A AD - Institute for Bioengineering of Catalonia (IBEC), Barcelona, 08028, Spain. FAU - Pradhan, Susav AU - Pradhan S AD - Raman Research Institute, C. V. Raman Avenue, Bengaluru, 560080, India. FAU - Sharma, Sumit AU - Sharma S AD - CSIR - Indian Institute of Integrative Medicine, Jammu, 180001, India. FAU - Singh, Parvinder P AU - Singh PP AD - CSIR - Indian Institute of Integrative Medicine, Jammu, 180001, India. FAU - Guadamillas, Marta C AU - Guadamillas MC AD - Integrin Signalling Lab, Cell Biology & Physiology Program, Cell & Developmental Biology Area, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid, 28029, Spain. FAU - Chaudhary, Natasha AU - Chaudhary N AD - University of Queensland, Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, St Lucia, QLD, 4072, Australia. AD - Department of Biochemistry, Weill Cornell Medical College, New York, NY, 10065, USA. FAU - Vishwakarma, Ram AU - Vishwakarma R AD - CSIR - Indian Institute of Integrative Medicine, Jammu, 180001, India. FAU - Trepat, Xavier AU - Trepat X AUID- ORCID: 0000-0002-7621-5214 AD - Institute for Bioengineering of Catalonia (IBEC), Barcelona, 08028, Spain. AD - University of Barcelona, Barcelona, 08036, Spain. AD - Centro de Investigacion Biomedica en Red en Bioingenieria, Biomateriales y Nanomedicina (CIBER-BBN) and Institucio Catalana de Recerca i Estudis Avancats (ICREA), Barcelona, 08010, Spain. FAU - Del Pozo, Miguel A AU - Del Pozo MA AUID- ORCID: 0000-0001-9077-391X AD - Integrin Signalling Lab, Cell Biology & Physiology Program, Cell & Developmental Biology Area, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid, 28029, Spain. FAU - Parton, Robert G AU - Parton RG AUID- ORCID: 0000-0002-7494-5248 AD - University of Queensland, Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, St Lucia, QLD, 4072, Australia. FAU - Rao, Madan AU - Rao M AD - National Centre for Biological Sciences (NCBS), Tata Institute of Fundamental Research (TIFR), Bellary Road, Bengaluru, 560065, India. AD - Simons Centre for the Study of Living Machines, National Centre for Biological Sciences (NCBS), Bengaluru, 560065, India. FAU - Pullarkat, Pramod AU - Pullarkat P AD - Raman Research Institute, C. V. Raman Avenue, Bengaluru, 560080, India. FAU - Roca-Cusachs, Pere AU - Roca-Cusachs P AUID- ORCID: 0000-0001-6947-961X AD - Institute for Bioengineering of Catalonia (IBEC), Barcelona, 08028, Spain. AD - University of Barcelona, Barcelona, 08036, Spain. FAU - Mayor, Satyajit AU - Mayor S AD - National Centre for Biological Sciences (NCBS), Tata Institute of Fundamental Research (TIFR), Bellary Road, Bengaluru, 560065, India. mayor@ncbs.res.in. AD - Institute for Stem Cell Biology and Regenerative Medicine, Tata Institute of Fundamental Research (TIFR), Bengaluru, 560065, India. mayor@ncbs.res.in. LA - eng GR - Wellcome Trust/United Kingdom GR - IA/M/15/1/502018/WTDBT_/DBT-Wellcome Trust India Alliance/India PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20181011 PL - England TA - Nat Commun JT - Nature communications JID - 101528555 RN - 125361-02-6 (Vinculin) RN - EC 3.6.5.5 (Dynamins) SB - IM MH - Animals MH - Biomechanical Phenomena MH - Cell Adhesion MH - Cell Membrane/*metabolism MH - Dynamins/*metabolism MH - *Endocytosis MH - *Feedback, Physiological MH - *Mechanotransduction, Cellular MH - Mice MH - Signal Transduction MH - Temperature MH - Vinculin/metabolism PMC - PMC6181995 COIS- The authors declare no competing interests. EDAT- 2018/10/13 06:00 MHDA- 2019/01/08 06:00 PMCR- 2018/10/11 CRDT- 2018/10/13 06:00 PHST- 2017/10/23 00:00 [received] PHST- 2018/09/04 00:00 [accepted] PHST- 2018/10/13 06:00 [entrez] PHST- 2018/10/13 06:00 [pubmed] PHST- 2019/01/08 06:00 [medline] PHST- 2018/10/11 00:00 [pmc-release] AID - 10.1038/s41467-018-06738-5 [pii] AID - 6738 [pii] AID - 10.1038/s41467-018-06738-5 [doi] PST - epublish SO - Nat Commun. 2018 Oct 11;9(1):4217. doi: 10.1038/s41467-018-06738-5.